Template:Team:Groningen/CONTENT/LOGBOOK/Psalt Digestion zp14

Psalt Digestion (zp14)

Digestion is performed to prepare DNA for ligation. Psalt and pSB1C3 are digested to ligate the salt inducible promotor into a backbone.

To create a salt inducible promotor.

Digestion was done.

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>

00:00, 13 July 2015 - 00:00, 13 July 2015

The salt promotor and backbone are digested with EcoRI and PstI.

Marieke Mulder