Template:Team:Groningen/CONTENT/LOGBOOK/Psalt Ligation zp12

Psalt Ligation (zp12)

Ligation was done to combine the salt inducible promotor (proH) into a backbone in order to put it in front of a gene.

To create a salt inducible promotor.

Ligation was not successful.

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>

00:00, 10 July 2015 - 00:00, 10 July 2015

Ligation of proH promoter in K823023 and also in pSB1C3. Used 10 ul proH and 5 ul backbone (K823023 and pSB1C3)

Marieke Mulder