PCR was performed with the forward and reverse primer for Psalt (figure 1) and a annealing temperature of 40°C. PCR product was checked and purified on 1% agarose gel with 1:50000 SERVA DNA Stain G. Gel results show a band at approximately 600bp.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt PCR zp5
Psalt PCR (zp5)
A PCR is done to get the DNA of the salt inducible promotor out of the genomen of B. subtilis 168.
To create a salt inducible promotor.
PCR was successful.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/PCR">PCR</a>
00:00, 30 June 2015 - 00:00, 30 June 2015
Primer
Sequence
Forward
GCGGGGGAATTCGCGGCCGCTTCTAGAGGTCTGAATTTAAAGAGATTG
Reverse
GAGCTTTACTAGTAGCGGCCGCTGCAGGATCCTGCTCCTATAAAGGCTAC
Primer sequences