Competent E. coli were transformed with 10 µL ligation product and plated on LB with ampicillin (K823023) and LB with chloramphenicol (pSB1C3). Transformation failed. No white colonies visible on plate.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt Transformation zp13
Psalt Transformation (zp13)
Transformation was done to introduce the ligated plasmid DNA into E. coli.
To create a salt inducible promotor.
Transformation was not successful.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Heat_shock_transformation">Heat shock transformation</a>
00:00, 10 July 2015 - 00:00, 10 July 2015