Competent E. coli were transformed with 10 µL ligation product and plated on LB with ampicillin. Transformation failed. No white colonies visible on plate.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt Transformation zp4
Psalt Transformation (zp4)
Transformation was done to introduce the ligated plasmid DNA into E. coli.
To create a salt inducible promotor.
Transformation was not successful.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Heat_shock_transformation">Heat shock transformation</a>
00:00, 29 June 2015 - 00:00, 30 June 2015