Template:Team:Groningen/CONTENT/LOGBOOK/Purification a36
Purification_a36
Full genome purification of BD1807 strain which has the abrB knockout
To knock out abrB in Bacillus subtilis strains to obtain a more rigid biofilm. AbrB suppresses biofilm formation. Knocking out AbrB should result in a more rigid biofilm.
PCR succeeded, as bands were found on agarose gel.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/PCR">PCR</a>
00:00, 25 July 2015 - 00:00, 25 July 2015
Full genome purification of BD1807 strain, which has the abrB knockout, according to protocol
PCR succeeded as bands at the right height were found on agarose gel.