Template:Team:Groningen/CONTENT/LOGBOOK/Restriction pg12
Restriction (pg12)
Restriction is performed to be able to ligate the PGA DNA into a backbone. This has to be done to be able to transform it into E. coli.
Make a more negatively charged biofilm.
Restriction done.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 22 July 2015 - 00:00, 22 July 2015
Restriction of PGA with EcoRI and PstI. 7µL of PGA was used.