Template:Team:Groningen/CONTENT/LOGBOOK/Restriction th3
Restriction (th3)
Digestion of pdg1664
Extra integration of DNA in thrC locus
Plasmid was digested and purified
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 26 Augustus 2015 - 00:00, 26 Augustus 2015
pdg1664 plasmid DNA was digested with EcoRI and AatII for 2 hours at 37 °C. The restriction samples were run on a 1% agarose gel and correct bands were cut out of the gel and purified with the PCR purification kit from Thermo Scientific.