Template:Team:Groningen/CONTENT/LOGBOOK/Transformation fr6
Transformation ferritin ligated in pSB1C3 (fr6)
Transformation of ferritin ligated with pSB1C3 in E. coli
Development ferritin
Transformed cells
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Heat_shock_transformation">Heat shock transformation</a>
22:30, 31 August 2015 - 00:30, 1 September 2015
Ligation product (30 µL) was transformed into 10 µL NEB DH5 alpha competent cells. Cells were plated on LB + chloramphenicol agar plates.