Template:Team:Groningen/CONTENT/LOGBOOK/Transformation with pSB1C3 pg26
Transformation with pSB1C3 (pg26)
Transformation was done to check if ligation was successful.
Make a more negatively charged biofilm.
Transformation was successful. A few white colonies visible.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Heat_shock_transformation">Heat shock transformation</a>
00:00, 31 July 2015 - 00:00, 31 July 2015
Competent E. coli were transformed with ligation product of PGA with pSB1C3 and plated on LB plates with chloramphenicol.