Template:Team:Groningen/CONTENT/LOGBOOK/tasA Ligation t22
tasA K823023 Ligation (t22)
In this experiment tasA was ligated with the K823023 backbone to make transformation possible.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The K823023 backbone was ligated with tasA.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>
00:00, 3 June 2015 - 00:00, 3 June 2015
For the ligation of tasA in BBa_K823023, the following components were used.
Component
Amount
Vector DNA (K823023)
2 µL
Insert DNA (tasA)
2 µL
10x buffer
2 µL
T4 ligase
1 µL
\( \mathrm{H_2O}\)
13 µL
Components used for ligation.
The ligation mixture was left 2 hours at room temperature.