Template:Team:Groningen/CONTENT/LOGBOOK/tasA PCR purification t3
tasA PCR purification (t3)
A PCR purification was performed to get rid of the enzymes used in the PCR process.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The PCR product was purified.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/PCRpurification">PCR purification</a>
00:00, 13 May 2015 - 00:00, 13 May 2015
The PCR products were PCR purified according to protocol. The PCR purification products of samples 3, 4 and 5 were stored in freezer at -20 °C as 1.3, 1.4 and 1.5.