Template:Team:Groningen/CONTENT/LOGBOOK/tasA PCR purification t8
tasA PCR purification (t8)
The Gibson assembly products were purified.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The purification of the Gibson assembly products was performed.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/PCRpurification">PCR purification</a>
00:00, 19 May 2015 - 00:00, 19 May 2015
The Gibson assembly products were purified with the PCR purification kit and stored as tasA DNA 1 and tasA DNA 2 in freezer at -20 °C.