LOCUS       pET_1703                6033 bp    DNA     circular UNA 11-AUG-2015
DEFINITION  HIV antigen with 10xHis-tag.
ACCESSION   urn.local...1x-50im4eu
VERSION     urn.local...1x-50im4eu
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    387..405
     misc_feature    406..430
     misc_feature    475..540
     gene            574..1110
     unsure          1120..1149
     misc_feature    1232..1278
     rep_origin      1315..1770
     CDS             1902..2759
                     /note="Geneious type: cds"
     rep_origin      2917..3536
     CDS             complement(4954..6033)
                     /note="Geneious type: cds"
ORIGIN      
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
      181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
      241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
      301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
      361 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      421 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat acatatgaaa
      481 tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc
      541 atggatatcg gaattaattc gggtaccgga tccgggatta gctatggccg caagaaacgt
      601 cgccaacgtc gtcgtgcgca tcagaacatg gaaccagtag acccgcgtct ggagccgtgg
      661 aaacatccgg gctctcagcc gaaaaccgca gcgtatccgc aaggatggaa aggaagcccg
      721 gcgattttcc agtctagcat gaccaaaatc ctggagccat tccggaaaca aaacccggac
      781 attgtcattt atcaatacat ggatgatttg tacgtgggtg cggcagcgaa ggagccgttc
      841 cgtgattacg tagatcgttt ttataaaacc cttcgggctg aacaagcaag ccaggaagtt
      901 aagaactgga tgacggcggc ctatcaggct cgcattttgg cggtagaacg ttacctgaaa
      961 gaccaacagc tgctgggcat ttggggctgt tcaggaaagc tgatctgcac gactgcggtg
     1021 ccgtggtgta cgcgtccgaa caacaacacc cgcaaacgta tccgtattca acggggtcct
     1081 ggtcgcgcgt tcgtaaccat tggaaagatc aagcttggcc atcaccatca ccatcaccat
     1141 caccatcact agcttaagtt gagatgagat ccggctgcta acaaagcccg aaaggaagct
     1201 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     1261 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
     1321 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     1381 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     1441 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     1501 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     1561 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     1621 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     1681 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     1741 attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg
     1801 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1861 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1921 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1981 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2041 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2101 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2161 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2221 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2281 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2341 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2401 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     2461 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2521 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2581 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2641 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2701 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2761 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2821 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2881 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2941 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3001 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3061 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3121 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3181 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3241 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3301 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3361 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3421 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3481 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3541 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3601 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3661 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     3721 ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat
     3781 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     3841 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     3901 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     3961 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     4021 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     4081 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     4141 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     4201 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     4261 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     4321 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     4381 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     4441 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     4501 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     4561 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     4621 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     4681 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     4741 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     4801 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     4861 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     4921 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4981 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5041 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     5101 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     5161 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     5221 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     5281 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     5341 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     5401 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     5461 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     5521 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     5581 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     5641 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     5701 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     5761 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     5821 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     5881 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     5941 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     6001 ctctgcgaca tcgtataacg ttactggttt cac
//
