LOCUS       BBa_K1621001            3618 bp    DNA     circular UNA 07-SEP-2015
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...199-5072ex7
VERSION     urn.local...199-5072ex7
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     112..131
     misc_feature    230..250
     gene            253..1800
     misc_feature    1801..1820
     stem_loop       1821..1892
                     /note="Geneious type: misc_feature"
     misc_feature    complement(1954..1973)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2060..2674)
                     /note="Geneious type: misc"
                     /gene="rep (pMB1)"
                     /note="Geneious type: misc_feature"
     stem_loop       2841..2946
                     /note="Geneious type: misc_feature"
     misc_feature    complement(2959..3618)
                     /note="Geneious type: CDS; cds"
                     /gene="Chloramphenicol resistance"
                     /note="Geneious type: CDS"
ORIGIN      
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga tgacgaatcc cgtcagagcg tctgtgctga gatatgacga ttttcacacc
      301 gatgaagata aactcgacac caatagcgtg tacgaaccat actaccacag cgatcatgcg
      361 gaaagctctt gggtgaatag gggtgagtcc tccagaaagg cttacgacca taattcacct
      421 tacatttggc ccaggaacga ctacgacggc tttctcgaga atgcccacga gcaccacggc
      481 gtgtacaacc agggtcgggg gatcgactca ggggagcggc tgatgcagcc cacacagatg
      541 tctgctcagg aggacctggg cgatgatacc ggcatccacg tgattccgac tctgaacggg
      601 gatgaccgcc acaagatcgt aaatgtcgac caaaggcagt atggagacgt attcaaaggg
      661 gaccttaacc ctaaacctca gggccagcgc ctgatagagg tctccgtgga ggaaaaccac
      721 ccttttaccc ttagagctcc cattcagaga atttacggag tgcgctacac cgaaacatgg
      781 agcttcctcc ccagtctcac ttgcaccggc gacgccgcgc cagccatcca gcacatttgt
      841 ctgaaacaca ccacctgttt ccaggacgtt gttgtggacg tagattgtgc tgagaacaca
      901 aaggaggatc agttggctga aatctcctat cgctttcagg gcaagaagga ggccgaccag
      961 ccttggattg tggtaaacac ctccacgctg tttgatgagc ttgagctcga ccctcctgag
     1021 atcgagcctg gtgtactgaa agtgctccgc acagaaaagc agtatttggg ggtgtacatc
     1081 tggaacatga ggggcagtga tggcacttcc acctatgcca ctttcctggt cacctggaaa
     1141 ggcgacgaga agaccaggaa ccccacacca gcggtcacac ctcaaccaag aggggccgag
     1201 tttcacatgt ggaattacca ctcccacgtg ttcagtgtcg gtgacacatt ctccctggct
     1261 atgcatctgc aatacaaaat ccatgaggcg ccttttgatc tgctcctcga atggctctac
     1321 gtgcccattg accccacttg tcagccaatg aggctctatt ccacctgctt gtaccaccca
     1381 aacgcacccc agtgtcttag ccatatgaac tctggatgta cattcacgag tccgcatctt
     1441 gcccaacgcg ttgcatctac cgtgtaccag aactgtgagc atgccgataa ctacaccgcg
     1501 tattgcctgg gcatcagcca catggagccc agtttcggac tgatacttca cgacggcgga
     1561 acaacactga agttcgtgga cacaccagag agtctctctg gcctgtacgt gttcgtggtg
     1621 tattttaacg gacatgtgga ggccgtggct tacacagtgg tcagcaccgt ggaccatttt
     1681 gtgaatgcca tcgaggaacg ggggttccca cccaccgcag ggcagcctcc tgcaacaaca
     1741 aagcccaagg aaatcacccc cgttaatccc gggacatctc ccttgttgcg atatgccgcc
     1801 actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
     1861 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
     1921 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1981 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2041 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     2101 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2161 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2221 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2281 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2341 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2401 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2461 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2521 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2581 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2641 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2701 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2761 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2821 tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     2881 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     2941 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     3001 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     3061 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     3121 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     3181 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     3241 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     3301 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     3361 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     3421 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     3481 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     3541 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     3601 agtgattttt ttctccat
//
