LOCUS       pET_1103                6849 bp    DNA     circular UNA 11-AUG-2015
DEFINITION  Tetanus antigen with 10xHis-tag.
ACCESSION   urn.local...w7-506ynit
VERSION     urn.local...w7-506ynit
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    387..405
     misc_feature    406..430
     misc_feature    475..540
     gene            574..1926
     unsure          1936..1965
     misc_feature    2048..2094
     rep_origin      2131..2586
     CDS             2718..3575
                     /note="Geneious type: cds"
     rep_origin      3733..4352
     CDS             complement(5770..6849)
                     /note="Geneious type: cds"
ORIGIN      
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
      181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
      241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
      301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
      361 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      421 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat acatatgaaa
      481 tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc
      541 atggatatcg gaattaattc gggtaccgga tccaaaaatt tagattgctg ggtggataat
      601 gaggaagata ttgatgtcat tctgaaaaaa agcactattt tgaatctgga tatcaacaac
      661 gatatcatct ccgatatctc tggcttcaat tcctcggtaa tcacatatcc ggacgcacag
      721 ctggttccgg gcatcaacgg taaggccatt cacttggtga acaacgagtc gagcgaggtt
      781 attgtccaca aagctatgga tatcgaatat aacgatatgt ttaataactt tacggtgagc
      841 ttctggctgc gcgtgccaaa agtaagtgca tcccatttag aacagtacga caccaacgaa
      901 tacagtatta tttccagtat gaagaaatat tcgctttcga tcggctcagg atggagtgta
      961 agtcttaaag gcaacaatct tatttggacg ttgaaagata gcgccggcga agtgcgccag
     1021 attacatttc gcgacttgag tgataaattt aacgcatacc tggcaaacaa gtgggtattc
     1081 attactatta ccaatgatcg cctgagtagt gccaacctgt acatcaacgg cgtattgatg
     1141 ggttccgcag aaattaccgg cttgggtgcc atccgtgaag ataacaatat cactttgaaa
     1201 ctcgaccgct gcaacaataa taaccaatat gtatcgatcg ataaattccg gatcttttgt
     1261 aaagctctta acccgaaaga gattgagaaa ctctacacgt catatttgtc cattaccttc
     1321 ctgcgtgatt tctggggcaa tccgctgcgg tacgacaccg aatactacct tattcctgtc
     1381 gcctatagta gtaaagatgt ccagttaaaa aacattacag attatatgta ccttaccaac
     1441 gcgccttcgt acaccaacgg gaagctgaac atttattacc gccgtctgta cagcggtctg
     1501 aaatttatca ttaaacggta tacgcctaac aatgagattg actccttcgt ccggtcgggg
     1561 gactttatca aattgtatgt ttcatataat aacaacgaac acattgtcgg ctacccaaaa
     1621 gatggaaacg cgtttaataa cctcgaccgc attctgcgtg tcggctacaa cgcgccagga
     1681 atcccgttat acaagaaaat ggaagccgtc aaactccgcg atcttaaaac ttattcagtg
     1741 cagcttaaac tctacgatga caaagatgct tcactgggtt tagtcggaac tcataacggc
     1801 cagatcggca acgatccaaa ccgcgacatc ttaatcgcgt cgaactggta ttttaaccac
     1861 ctgaaagata aaaccctgac ctgtgattgg tatttcgtcc ctaccgatga aggctggacc
     1921 aatgataagc ttggccatca ccatcaccat caccatcacc atcactagct taagttgaga
     1981 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     2041 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     2101 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     2161 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     2221 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2281 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2341 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     2401 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     2461 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     2521 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     2581 caatttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2641 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2701 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2761 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2821 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2881 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2941 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3001 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3061 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3121 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3181 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3241 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3301 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3361 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3421 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3481 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3541 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3601 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3661 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3721 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3781 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3841 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3901 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3961 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4021 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4081 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4141 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4201 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4261 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4321 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4381 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4441 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4501 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4561 tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4621 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     4681 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     4741 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4801 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     4861 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     4921 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     4981 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     5041 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     5101 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
     5161 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     5221 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     5281 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     5341 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     5401 aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg
     5461 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
     5521 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
     5581 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
     5641 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     5701 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
     5761 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5821 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
     5881 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     5941 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
     6001 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac
     6061 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
     6121 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
     6181 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
     6241 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
     6301 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
     6361 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
     6421 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
     6481 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
     6541 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
     6601 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
     6661 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
     6721 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
     6781 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
     6841 tggtttcac
//
