LOCUS       pET_1104                6882 bp    DNA     circular UNA 11-AUG-2015
DEFINITION  Tetanus antigen with Spy- and 6xHis-tag.
ACCESSION   urn.local...105-506yxtk
VERSION     urn.local...105-506yxtk
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    387..405
     misc_feature    406..430
     misc_feature    475..540
     unsure          571..588
     gene            598..1950
     unsure          1960..1998
     misc_feature    2081..2127
     rep_origin      2164..2619
     CDS             2751..3608
                     /note="Geneious type: cds"
     rep_origin      3766..4385
     CDS             complement(5803..6882)
                     /note="Geneious type: cds"
ORIGIN      
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
      181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
      241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
      301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
      361 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      421 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat acatatgaaa
      481 tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc
      541 atggatatcg gaattaattc gggtaccatg catcaccatc accatcacgg cggatccaaa
      601 aatttagatt gctgggtgga taatgaggaa gatattgatg tcattctgaa aaaaagcact
      661 attttgaatc tggatatcaa caacgatatc atctccgata tctctggctt caattcctcg
      721 gtaatcacat atccggacgc acagctggtt ccgggcatca acggtaaggc cattcacttg
      781 gtgaacaacg agtcgagcga ggttattgtc cacaaagcta tggatatcga atataacgat
      841 atgtttaata actttacggt gagcttctgg ctgcgcgtgc caaaagtaag tgcatcccat
      901 ttagaacagt acgacaccaa cgaatacagt attatttcca gtatgaagaa atattcgctt
      961 tcgatcggct caggatggag tgtaagtctt aaaggcaaca atcttatttg gacgttgaaa
     1021 gatagcgccg gcgaagtgcg ccagattaca tttcgcgact tgagtgataa atttaacgca
     1081 tacctggcaa acaagtgggt attcattact attaccaatg atcgcctgag tagtgccaac
     1141 ctgtacatca acggcgtatt gatgggttcc gcagaaatta ccggcttggg tgccatccgt
     1201 gaagataaca atatcacttt gaaactcgac cgctgcaaca ataataacca atatgtatcg
     1261 atcgataaat tccggatctt ttgtaaagct cttaacccga aagagattga gaaactctac
     1321 acgtcatatt tgtccattac cttcctgcgt gatttctggg gcaatccgct gcggtacgac
     1381 accgaatact accttattcc tgtcgcctat agtagtaaag atgtccagtt aaaaaacatt
     1441 acagattata tgtaccttac caacgcgcct tcgtacacca acgggaagct gaacatttat
     1501 taccgccgtc tgtacagcgg tctgaaattt atcattaaac ggtatacgcc taacaatgag
     1561 attgactcct tcgtccggtc gggggacttt atcaaattgt atgtttcata taataacaac
     1621 gaacacattg tcggctaccc aaaagatgga aacgcgttta ataacctcga ccgcattctg
     1681 cgtgtcggct acaacgcgcc aggaatcccg ttatacaaga aaatggaagc cgtcaaactc
     1741 cgcgatctta aaacttattc agtgcagctt aaactctacg atgacaaaga tgcttcactg
     1801 ggtttagtcg gaactcataa cggccagatc ggcaacgatc caaaccgcga catcttaatc
     1861 gcgtcgaact ggtattttaa ccacctgaaa gataaaaccc tgacctgtga ttggtatttc
     1921 gtccctaccg atgaaggctg gaccaatgat aagcttggcg ctcatattgt catggttgat
     1981 gcttacaagc caactaagta gcttaagttg agatgagatc cggctgctaa caaagcccga
     2041 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     2101 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
     2161 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2221 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2281 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2341 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2401 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2461 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2521 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2581 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc aggtggcact tttcggggaa
     2641 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2701 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2761 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2821 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2881 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2941 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3001 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3061 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3121 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3181 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3241 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     3301 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3361 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3421 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3481 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3541 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3601 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3661 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3721 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3781 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3841 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3901 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3961 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4021 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4081 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4141 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4201 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4261 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4321 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4381 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4441 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4501 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     4561 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct
     4621 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     4681 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4741 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4801 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     4861 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     4921 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     4981 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     5041 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     5101 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     5161 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
     5221 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     5281 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     5341 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     5401 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggg
     5461 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg
     5521 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
     5581 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
     5641 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc
     5701 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     5761 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5821 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5881 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     5941 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     6001 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     6061 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
     6121 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     6181 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     6241 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     6301 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     6361 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     6421 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     6481 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     6541 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     6601 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     6661 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     6721 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     6781 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     6841 accggcatac tctgcgacat cgtataacgt tactggtttc ac
//
