LOCUS AH009 6973 bp ds-DNA linear 27-AUG-2015 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 3457..3457 /label=2nd construct ends /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1957..1957 /label=CAR /ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1958..1986 /label=CAR(1) /ApEinfo_label=CAR /ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 659..1333 /label=ppt /ApEinfo_fwdcolor=#ff8040 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3457..3457 /label=2nd construct ends(1) /ApEinfo_label=2nd construct ends /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=#ff0080 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 88..108 /label=Lac Operator /ApEinfo_fwdcolor=#85dae9 /ApEinfo_revcolor=#85dae9 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1349..1354 /label=RBS /ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3458..3458 /label=CAR(2) /ApEinfo_label=CAR /ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 promoter 67..86 /label=Promoter /ApEinfo_fwdcolor=#d59687 /ApEinfo_revcolor=#d59687 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(1362..1819,1821..1955,1957..1957) /label=CAR(3) /ApEinfo_label=CAR /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3428..3457 /label=CAR(4) /ApEinfo_label=CAR /ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1987..1987 /label=CAR(5) /ApEinfo_label=CAR /ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 142..147 /label=RBS(1) /ApEinfo_label=RBS /ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1987..1987 /label=1st construct ends /ApEinfo_fwdcolor=#0080c0 /ApEinfo_revcolor=#0080c0 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 terminator 4922..4969 /label=T7 terminator /ApEinfo_fwdcolor=#b1ff67 /ApEinfo_revcolor=#b1ff67 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1988..1988 /label=CAR(6) /ApEinfo_label=CAR /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 45..66 /label=Prefix /ApEinfo_fwdcolor=#85dae9 /ApEinfo_revcolor=#85dae9 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature join(1989..2506,2508..3425) /label=optimized car /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3459..4886 /label=CAR(7) /ApEinfo_label=CAR /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 156..620 /label=YciA /ApEinfo_fwdcolor=#ffff80 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3428..4969 /label=New Feature /ApEinfo_fwdcolor=#ff0080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 645..650 /label=RBS(2) /ApEinfo_label=RBS /ApEinfo_fwdcolor=#ff9ccd /ApEinfo_revcolor=#ff9ccd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3426..3427 /label=CAR(8) /ApEinfo_label=CAR /ApEinfo_fwdcolor=#ff80c0 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt 61 ctagagtaat acgactcact ataggggaat tgtgagcgga taacaattcc cctgtagaaa 121 taattttgtt taactttaag aaggagataa aaaaaATGTC GGCAAACTTT ACCGATAAAA 181 ACGGACGTCA GTCAAAAGGC GTCTTATTAT TGCGCACGCT GGCTATGCCT AGCGATACAA 241 ATGCCAACGG CGACATTTTC GGGGGGTGGA TTATGTCTCA GATGGACATG GGTGGTGCGA 301 TTCTTGCTAA AGAAATTGCA CATGGCCGCG TGGTTACAGT GGCTGTCGAA AGCATGAATT 361 TTATCAAGCC GATTTCCGTA GGCGATGTAG TGTGCTGTTA CGGTCAGTGC CTGAAGGTGG 421 GGCGCAGCTC GATTAAAATT AAAGTGGAGG TATGGGTCAA AAAGGTGGCC TCGGAACCCA 481 TTGGCGAACG TTATTGCGTG ACGGATGCGG TATTCACGTT TGTCGCAGTA GATAATAACG 541 GTCGTAGCCG GACTATTCCC CGTGAAAACA ACCAGGAACT GGAAAAAGCG CTTGCGTTGA 601 TCAGTGAGCA GCCACTGTAA taattaatta aaaagcttgg atccaggagg taaaaaaaAT 661 GAAAATCTAC GGCATCTATA TGGATCGTCC GCTGAGCCAA GAAGAAAACG AACGTTTTAT 721 GAGCTTTATC AGTCCGGAAA AACGTGAAAA ATGCCGTCGC TTTTATCACA AAGAAGATGC 781 ACATCGTACC CTGCTGGGTG ATGTTCTGGT TCGTAGCGTT ATTAGCCGTC AGTATCAGCT 841 GGATAAATCC GATATTCGTT TTAGCACCCA AGAATATGGT AAACCGTGTA TTCCGGATCT 901 GCCGGATGCA CATTTTAACA TTAGCCATAG CGGTCGTTGG GTTATTTGTG CATTTGATAG 961 CCAGCCGATT GGCATTGATA TCGAAAAAAC CAAACCGATC AGCCTGGAAA TTGCCAAACG 1021 TTTTTTTAGC AAAACCGAGT ATAGCGATCT GCTGGCCAAA GATAAAGATG AACAGACCGA 1081 TTATTTCTAT CATCTGTGGT CCATGAAAGA GAGCTTCATT AAACAAGAAG GTAAAGGTCT 1141 GAGCCTGCCG CTGGATAGCT TTAGCGTTCG TCTGCATCAG GATGGTCAGG TTAGCATTGA 1201 ACTGCCGGAT AGCCATAGTC CGTGTTATAT CAAAACCTAT GAAGTTGACC CTGGCTACAA 1261 AATGGCAGTT TGTGCAGCAC ATCCGGATTT TCCGGAAGAT ATTACCATGG TTAGCTATGA 1321 AGAACTGCTG TAAtaaggat cccaattcag gaggtaaaaa aATGAGTCCG ATTACACGCG 1381 AGGAACGTCT GGAGCGCCGT ATCCAGGACC TGTACGCGAA CGATCCGCAG TTTGCCGCGG 1441 CCAAGCCGGC GACCGCGATT ACAGCCGCCA TCGAACGCCC TGGTCTGCCG CTTCCGCAGA 1501 TCATTGAAAC CGTGATGACC GGGTACGCGG ATCGTCCAGC GTTGGCGCAG CGCTCCGTCG 1561 AGTTTGTGAC TGATGCAGGC ACGGGCCATA CTACGCTGCG GCTTCTGCCT CACTTTGAAA 1621 CGATTTCGTA TGGCGAACTT TGGGATCGCA TTAGCGCATT AGCAGACGTC CTGAGCACTG 1681 AACAGACGGT CAAACCGGGC GATCGCGTCT GTCTTCTTGG CTTCAACTCC GTCGATTATG 1741 CAACCATCGA TATGACCCTT GCTCGCCTGG GCGCAGTCGC CGTGCCCTTG CAGACATCCG 1801 CTGCGATTAC GCAGCTGCAA CCAATCGTAG CTGAAACCCA GCCGACCATG ATCGCTGCAT 1861 CTGTGGACGC ACTGGCTGAC GCCACAGAAC TGGCACTTAG CGGTCAGACG GCGACCCGCG 1921 TACTGGTGTT TGATCACCAT CGTCAGGTTG ACGCAcatcg tgcggcggtt gaaagcgcgc 1981 gtgaacgTCT GGCAGGTTCT GCGGTGGTGG AGACGTTGGC AGAGGCGATT GCACGCGGTG 2041 ATGTTCCGCG GGGCGCCTCT GCTGGCTCGG CGCCTGGTAC TGATGTTAGT GACGACAGCC 2101 TGGCACTGCT CATCTATACT TCGGGCAGTA CAGGGGCTCC GAAAGGCGCA ATGTATCCGC 2161 GTCGTAACGT TGCTACCTTT TGGCGCAAAC GTACGTGGTT TGAAGGTGGT TATGAACCTA 2221 GCATCACGCT GAACTTCATG CCCATGAGCC ATGTCATGGG TCGTCAAATC CTTTATGGGA 2281 CCCTCTGCAA CGGCGGTACA GCGTACTTCG TAGCGAAATC GGACCTGAGT ACATTATTTG 2341 AGGACCTGGC CCTGGTACGG CCGACCGAGC TGACTTTTGT GCCACGCGTG TGGGACATGG 2401 TCTTTGACGA GTTCCAGAGT GAGGTCGATC GCCGTCTGGT AGATGGCGCA GATCGTGTTG 2461 CACTGGAGGC CCAGGTTAAA GCAGAAATTC GGAATGACGT TCTCGGTGGC CGCTACACGA 2521 GCGCGCTGAC TGGCAGCGCC CCGATTAGTG ATGAGATGAA GGCCTGGGTG GAGGAGTTAC 2581 TCGACATGCA TCTGGTAGAA GGCTATGGCA GCACTGAGGC GGGCATGATC CTGATCGACG 2641 GCGCAATTCG CCGCCCAGCC GTTCTGGACT ATAAACTCGT CGACGTACCG GATCTCGGCT 2701 ATTTTCTGAC CGATCGTCCG CACCCACGCG GTGAATTGCT GGTGAAGACC GATTCGTTGT 2761 TCCCGGGCTA TTATCAGCGG GCAGAAGTGA CGGCGGACGT GTTTGACGCC GATGGCTTCT 2821 ATCGGACAGG CGATATTATG GCGGAAGTGG GTCCCGAACA GTTCGTCTAT TTGGACCGTC 2881 GTAACAATGT TCTGAAGTTA AGTCAGGGTG AGTTCGTTAC TGTTTCAAAA CTTGAGGCAG 2941 TGTTTGGCGA TTCACCGTTA GTCCGCCAGA TCTATATCTA TGGGAATAGT GCTCGCGCCT 3001 ACTTACTCGC CGTGATTGTT CCGACACAAG AAGCCCTGGA TGCGGTGCCC GTTGAAGAAC 3061 TCAAGGCACG GCTTGGTGAT TCGCTGCAAG AGGTGGCGAA GGCGGCGGGT CTGCAAAGCT 3121 ATGAAATTCC CCGCGATTTC ATCATTGAAA CGACGCCCTG GACGCTTGAA AATGGTCTGT 3181 TGACGGGCAT CCGCAAATTA GCCCGCCCGC AGCTCAAAAA ACACTATGGG GAACTTCTGG 3241 AGCAAATCTA TACCGATCTC GCTCATGGTC AAGCAGATGA GCTGCGGTCT TTACGCCAGA 3301 GTGGCGCGGA CGCTCCGGTA CTTGTGACCG TGTGTCGCGC CGCAGCGGCC CTTCTGGGCG 3361 GCAGCGCGTC GGATGTTCAG CCGGATGCTC ATTTTACTGA CCTTGGGGGC GACTCTCTGA 3421 GCGCTCTgag ctttaccaac ctgctgcatg aaatctttGA TATTGAAGTG CCGGTCGGCG 3481 TAATCGTTTC CCCGGCCAAC GATCTGCAAG CCTTGGCGGA TTATGTTGAA GCGGCCCGTA 3541 AACCCGGCAG CTCACGCCCG ACTTTTGCGT CAGTGCACGG CGCATCCAAC GGCCAGGTAA 3601 CGGAAGTTCA CGCTGGGGAC TTGTCGCTTG ATAAATTTAT TGATGCAGCG ACGCTTGCTG 3661 AAGCCCCACG CCTGCCGGCG GCGAATACCC AGGTCCGTAC GGTTCTGCTT ACAGGCGCCA 3721 CCGGATTCCT GGGCCGCTAC TTAGCTCTGG AGTGGCTCGA GCGTATGGAT CTGGTGGACG 3781 GTAAGCTGAT TTGTCTGGTA CGTGCTAAAT CTGACACCGA AGCCCGGGCA CGTCTGGACA 3841 AAACCTTTGA TTCGGGGGAT CCTGAACTGC TGGCGCATTA TCGGGCACTG GCTGGGGATC 3901 ATTTAGAGGT ACTGGCGGGC GACAAGGGCG AAGCGGACCT TGGTCTGGAC CGCCAGACGT 3961 GGCAACGCTT AGCGGATACC GTGGATCTGA TTGTGGATCC GGCTGCGCTG GTGAACCATG 4021 TTCTGCCGTA CTCACAATTA TTCGGCCCGA ACGCACTGGG GACCGCCGAA CTTCTCCGTC 4081 TTGCTTTGAC CAGCAAAATC AAACCCTACA GCTACACCTC CACCATTGGG GTTGCAGATC 4141 AAATTCCTCC ATCCGCCTTC ACCGAAGACG CAGACATTCG CGTGATTTCA GCAACCCGTG 4201 CAGTGGATGA TTCATACGCT AATGGTTACA GCAACTCTAA GTGGGCTGGT GAAGTCCTGC 4261 TGCGTGAAGC ACATGATCTG TGTGGTCTGC CTGTAGCGGT ATTCCGGTGT GATATGATCT 4321 TGGCGGATAC CACCTGGGCC GGTCAGTTAA ACGTGCCGGA TATGTTTACC CGTATGATTT 4381 TGAGCCTGGC TGCAACTGGC ATCGCCCCGG GTTCCTTTTA TGAACTTGCG GCAGATGGCG 4441 CACGTCAACG CGCACATTAC GATGGTCTGC CGGTCGAGTT TATTGCCGAA GCGATCTCTA 4501 CCCTGGGAGC CCAGAGTCAA GATGGCTTCC ATACATATCA CGTTATGAAT CCTTACGATG 4561 ACGGTATCGG GCTGGATGAA TTTGTTGACT GGCTGAACGA ATCGGGTTGC CCCATCCAGC 4621 GTATCGCAGA TTATGGTGAC TGGTTACAAC GTTTTGAAAC CGCCCTGCGG GCCTTACCAG 4681 ATCGCCAGCG TCACAGCTCA CTGCTGCCTC TGCTGCATAA CTATCGTCAA CCAGAACGCC 4741 CTGTTCGCGG CAGCATTGCC CCGACCGACC GCTTCCGCGC TGCCGTCCAG GAAGCAAAAA 4801 TTGGTCCGGA TAAAGATATT CCGCATGTTG GTGCACCTAT TATTGTCAAA TACGTAAGTG 4861 ATTTGCGCCT GTTAGGTTTA TTATAAtaat tccctaggct gctgccaccg ctgagcaata 4921 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgT ACTAGTAGCG 4981 GCCGCTGCAG tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac 5041 cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg 5101 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 5161 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 5221 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa 5281 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 5341 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 5401 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 5461 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 5521 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5581 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5641 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 5701 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5761 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 5821 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5881 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 5941 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 6001 gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca 6061 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg 6121 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt 6181 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc 6241 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc 6301 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa 6361 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca 6421 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat 6481 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc 6541 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca 6601 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag 6661 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc 6721 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt 6781 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt 6841 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt 6901 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 6961 cacgaggcag aat //