LOCUS       pET_1105                7767 bp    DNA     circular UNA 11-AUG-2015
DEFINITION  Tetanus antigen with Halo- and 6xHis-tag.
ACCESSION   urn.local...ye-506yss8
VERSION     urn.local...ye-506yss8
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    387..405
     misc_feature    406..430
     misc_feature    475..540
     unsure          571..588
     gene            598..1950
     gene            <1999..2886
     misc_feature    2966..3012
     rep_origin      3049..3504
     CDS             3636..4493
                     /note="Geneious type: cds"
     rep_origin      4651..5270
     CDS             complement(6688..7767)
                     /note="Geneious type: cds"
ORIGIN      
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
      181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
      241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
      301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
      361 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      421 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat acatatgaaa
      481 tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc
      541 atggatatcg gaattaattc gggtaccatg catcaccatc accatcacgg cggatccaaa
      601 aatttagatt gctgggtgga taatgaggaa gatattgatg tcattctgaa aaaaagcact
      661 attttgaatc tggatatcaa caacgatatc atctccgata tctctggctt caattcctcg
      721 gtaatcacat atccggacgc acagctggtt ccgggcatca acggtaaggc cattcacttg
      781 gtgaacaacg agtcgagcga ggttattgtc cacaaagcta tggatatcga atataacgat
      841 atgtttaata actttacggt gagcttctgg ctgcgcgtgc caaaagtaag tgcatcccat
      901 ttagaacagt acgacaccaa cgaatacagt attatttcca gtatgaagaa atattcgctt
      961 tcgatcggct caggatggag tgtaagtctt aaaggcaaca atcttatttg gacgttgaaa
     1021 gatagcgccg gcgaagtgcg ccagattaca tttcgcgact tgagtgataa atttaacgca
     1081 tacctggcaa acaagtgggt attcattact attaccaatg atcgcctgag tagtgccaac
     1141 ctgtacatca acggcgtatt gatgggttcc gcagaaatta ccggcttggg tgccatccgt
     1201 gaagataaca atatcacttt gaaactcgac cgctgcaaca ataataacca atatgtatcg
     1261 atcgataaat tccggatctt ttgtaaagct cttaacccga aagagattga gaaactctac
     1321 acgtcatatt tgtccattac cttcctgcgt gatttctggg gcaatccgct gcggtacgac
     1381 accgaatact accttattcc tgtcgcctat agtagtaaag atgtccagtt aaaaaacatt
     1441 acagattata tgtaccttac caacgcgcct tcgtacacca acgggaagct gaacatttat
     1501 taccgccgtc tgtacagcgg tctgaaattt atcattaaac ggtatacgcc taacaatgag
     1561 attgactcct tcgtccggtc gggggacttt atcaaattgt atgtttcata taataacaac
     1621 gaacacattg tcggctaccc aaaagatgga aacgcgttta ataacctcga ccgcattctg
     1681 cgtgtcggct acaacgcgcc aggaatcccg ttatacaaga aaatggaagc cgtcaaactc
     1741 cgcgatctta aaacttattc agtgcagctt aaactctacg atgacaaaga tgcttcactg
     1801 ggtttagtcg gaactcataa cggccagatc ggcaacgatc caaaccgcga catcttaatc
     1861 gcgtcgaact ggtattttaa ccacctgaaa gataaaaccc tgacctgtga ttggtatttc
     1921 gtccctaccg atgaaggctg gaccaatgat aagcttgcgg cggcgggcgc acctggtttc
     1981 agtagcatca gcgcgggttc cgaaatcggt actggctttc cattcgaccc ccattatgtg
     2041 gaagtcctgg gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg
     2101 ctgttcctgc acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt
     2161 gcaccgaccc atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca
     2221 gacctgggtt atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg
     2281 ggtctggaag aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg
     2341 gccaagcgca atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc
     2401 ccgacctggg acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc
     2461 gacgtcggcc gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg
     2521 ggtgtcgtcc gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat
     2581 cctgttgacc gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca
     2641 gcgaacatcg tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg
     2701 aagctgctgt tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg
     2761 gccaaaagcc tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa
     2821 gaagacaacc cggacctgat cggcagcgag atcgcgcgct ggctgtctac tctggagatt
     2881 tccggtctta agttgagatg agatccggct gctaacaaag cccgaaagga agctgagttg
     2941 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     3001 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
     3061 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3121 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3181 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3241 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3301 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3361 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3421 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3481 acaaaatatt aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3541 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     3601 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     3661 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     3721 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     3781 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     3841 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3901 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3961 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4021 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4081 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4141 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     4201 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     4261 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     4321 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     4381 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     4441 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     4501 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     4561 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4621 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     4681 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4741 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4801 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4861 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4921 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4981 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5041 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5101 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5161 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     5221 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     5281 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     5341 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     5401 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     5461 ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc
     5521 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     5581 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5641 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5701 tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat
     5761 tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat
     5821 gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat
     5881 gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg
     5941 atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt
     6001 aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag
     6061 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
     6121 atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg
     6181 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
     6241 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     6301 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg
     6361 ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg
     6421 agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga
     6481 aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg
     6541 ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag
     6601 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
     6661 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6721 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
     6781 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
     6841 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
     6901 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
     6961 cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
     7021 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
     7081 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
     7141 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact
     7201 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc
     7261 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac
     7321 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc
     7381 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc
     7441 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag
     7501 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact
     7561 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt
     7621 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
     7681 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc
     7741 gacatcgtat aacgttactg gtttcac
//
