LOCUS       pSB1C3_BBa_K8230        2105 bp    DNA     circular     18-SEP-2015
DEFINITION  High copy number BioBrick RFC[10] plasmid carrying chloramphenicol
            resistance.
ACCESSION   pSB1C3 (modified)
KEYWORDS    .
SOURCE      null
  ORGANISM  pSB1C3 cloning vector
            .
FEATURES             Location/Qualifiers
     primer_bind     112..131
     misc_feature    232..251
     gene            252..286
     misc_feature    288..307
     stem_loop       308..379
                     /note="Geneious type: misc_feature"
     misc_feature    complement(441..460)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(547..1161)
                     /note="Geneious type: misc"
                     /gene="rep (pMB1)"
                     /note="Geneious type: misc_feature"
     stem_loop       1328..1433
                     /note="Geneious type: misc_feature"
     misc_feature    complement(1446..2105)
                     /note="Geneious type: CDS; cds"
                     /gene="Chloramphenicol resistance"
                     /note="Geneious type: CDS"
ORIGIN      
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga gttgacagct agctcagtcc tagggattgt gctagctact agtagcggcc
      301 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
      361 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
      421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      541 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
      601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      961 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1201 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1261 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     1321 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1381 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1441 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1501 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1561 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1621 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     1681 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1741 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1801 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1861 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1921 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1981 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     2041 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     2101 tccat
//
