LOCUS       BBa_K1621005            2598 bp    DNA     circular UNA 07-SEP-2015
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...180-50725dx
VERSION     urn.local...180-50725dx
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     112..131
     misc_feature    230..250
     gene            253..780
     misc_feature    781..800
     stem_loop       801..872
                     /note="Geneious type: misc_feature"
     misc_feature    complement(934..953)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1040..1654)
                     /note="Geneious type: misc"
                     /gene="rep (pMB1)"
                     /note="Geneious type: misc_feature"
     stem_loop       1821..1926
                     /note="Geneious type: misc_feature"
     misc_feature    complement(1939..2598)
                     /note="Geneious type: CDS; cds"
                     /gene="Chloramphenicol resistance"
                     /note="Geneious type: CDS"
ORIGIN      
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga tgaacatgtg cacggacgga aaaaaatatc acagcacggc gacgtctgcg
      301 gccgtgggag cttctgcgcc gggcgtaccg gacgcccggg ctatcgcggc aatctgtgaa
      361 cagctgcgcc agcatgttgc tgacctgggt gtgctttata tcaaactcca caactaccac
      421 tggcatatct acggaattga atttaaacag gtgcatgaac tgctggaaga atattatgtg
      481 agcgtgactg aagcctttga taccattgcc gaacggctcc ttcaactcgg ggcgcaggcg
      541 ccagccagca tggcggaata cttagcgctg tctggtattg cagaggaaac ggagaaagag
      601 attacgatcg tttcggcgtt agcgcgcgtg aagcgtgatt ttgaatattt aagcacacgt
      661 ttttctcaga ctcaggtgct ggccgcggaa tcaggagacg ccgtaacgga cggtattatt
      721 accgatattc tgcgcacatt agggaaagca atttggatgc tgggcgcaac cttaaaagca
      781 actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt
      841 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
      901 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      961 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1021 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     1081 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1141 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1201 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1261 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1321 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1381 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1441 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1501 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1561 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1621 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1681 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1741 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1801 tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     1861 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     1921 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     1981 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     2041 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     2101 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     2161 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     2221 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     2281 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     2341 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     2401 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     2461 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     2521 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     2581 agtgattttt ttctccat
//
