LOCUS       pIG15_105               4004 bp    DNA     circular UNA 18-SEP-2015
DEFINITION  CellfEx: His10-linker-Yfp-Linker-Halo (proka).
ACCESSION   urn.local...ft-4ox5vv4 (modified)
VERSION     urn.local...ft-4ox5vv4
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     112..131
     misc_feature    232..251
     misc_feature    252..270
                     /note="Geneious type: promoter prokaryotic"
                     /note="Geneious type: misc_feature"
     unsure          290..306
                     /note="Geneious type: misc_feature"
     unsure          331..348
     CDS             385..>1113
     gene            <1162..2049
     terminator      2136..2182
                     /note="Geneious type: misc_feature"
     misc_feature    2186..2206
     stem_loop       2207..2278
                     /note="Geneious type: misc_feature"
     misc_feature    complement(2340..2359)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2446..3060)
                     /note="Geneious type: misc"
                     /gene="rep (pMB1)"
                     /note="Geneious type: misc_feature"
     stem_loop       3227..3332
                     /note="Geneious type: misc_feature"
     CDS             complement(3345..4004)
                     /note="Geneious type: cds"
                     /gene="Chloramphenicol resistance"
                     /note="Geneious type: CDS"
ORIGIN      
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga gtaatacgac tcactatagg ccactagaaa taattttgtt taactttaag
      301 aaggagatat acatgggtac ccatcaccac catcaccatc accatcacca cggcgccgat
      361 gacgatgata aggttccgga tccgatgtcg agtggggccc tgttatttca cgggaaaatt
      421 ccttatgttg ttgaaatgga aggtaacgtt gatggacaca catttagcat tcggggtaaa
      481 ggctacggcg acgcctcggt tggtaaagtc gacgcgcagt ttatttgtac gaccggagac
      541 gtgccggttc cttggtcgac gctggtaacc acgctgacgt atggcgctca atgttttgct
      601 aagtatggac ctgaactgaa agacttctac aagtcgtgta tgccagatgg gtacgtgcaa
      661 gaacgtacga ttacctttga aggtgacggc aatttcaaaa cgcgcgcaga ggtgacgttc
      721 gagaacggta gtgtatacaa tcgcgtcaaa ctgaatggtc aaggatttaa aaaagacggc
      781 catgtcttag gcaaaaacct ggagtttaac ttcaccccgc actgccttta tatttggggc
      841 gaccaggcga atcatggctt aaaatcggcc tttaaaatct gccatgaaat tactggctcc
      901 aaaggtgatt ttattgtcgc cgatcatacc cagatgaaca ctccaattgg gggcggcccg
      961 gtgcatgtac cggagtacca tcatatgtca tatcacgtga aactgtcgaa ggatgtaacg
     1021 gatcatcggg acaatatgtc cttaaaagaa acagtccgtg ccgtcgactg ccgcaaaacg
     1081 tacgatttcg atgcaggttc cggtgacacc tctaagcttg cggcggcggg cgcacctggt
     1141 ttcagtagca tcagcgcggg ttccgaaatc ggtactggct ttccattcga cccccattat
     1201 gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga tggcacccct
     1261 gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat catcccgcat
     1321 gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa atccgacaaa
     1381 ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt catcgaagcc
     1441 ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct gggtttccac
     1501 tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt catccgccct
     1561 atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc cttccgcacc
     1621 accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg tacgctgccg
     1681 atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga gccgttcctg
     1741 aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat cgccggtgag
     1801 ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca gtcccctgtc
     1861 ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga agccgctcgc
     1921 ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct gaatctgctg
     1981 caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc tactctggag
     2041 atttccggtc ttaagtaatg attaataatg agatccggct gctaacaaag cccgaaagga
     2101 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     2161 acgggtcttg aggggttttt tgtgatacta gtagcggccg ctgcagtccg gcaaaaaagg
     2221 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
     2281 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2341 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2401 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2461 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2521 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2581 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2641 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2701 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2761 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2821 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2881 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2941 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3001 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3061 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3121 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3181 caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa
     3241 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     3301 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
     3361 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     3421 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     3481 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     3541 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     3601 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     3661 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     3721 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     3781 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     3841 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     3901 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     3961 tgggatatat caacggtggt atatccagtg atttttttct ccat
//
