LOCUS       BBa_K1621003            3423 bp    DNA     circular UNA 07-SEP-2015
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...17k-5072262
VERSION     urn.local...17k-5072262
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     112..131
     misc_feature    230..250
     gene            253..1605
     misc_feature    1606..1625
     stem_loop       1626..1697
                     /note="Geneious type: misc_feature"
     misc_feature    complement(1759..1778)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1865..2479)
                     /note="Geneious type: misc"
                     /gene="rep (pMB1)"
                     /note="Geneious type: misc_feature"
     stem_loop       2646..2751
                     /note="Geneious type: misc_feature"
     misc_feature    complement(2764..3423)
                     /note="Geneious type: CDS; cds"
                     /gene="Chloramphenicol resistance"
                     /note="Geneious type: CDS"
ORIGIN      
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga tgaaaaattt agattgctgg gtggataatg aggaagatat tgatgtcatt
      301 ctgaaaaaaa gcactatttt gaatctggat atcaacaacg atatcatctc cgatatctct
      361 ggcttcaatt cctcggtaat cacatatccg gacgcacagc tggttccggg catcaacggt
      421 aaggccattc acttggtgaa caacgagtcg agcgaggtta ttgtccacaa agctatggat
      481 atcgaatata acgatatgtt taataacttt acggtgagct tctggctgcg cgtgccaaaa
      541 gtaagtgcat cccatttaga acagtacgac accaacgaat acagtattat ttccagtatg
      601 aagaaatatt cgctttcgat cggctcagga tggagtgtaa gtcttaaagg caacaatctt
      661 atttggacgt tgaaagatag cgccggcgaa gtgcgccaga ttacatttcg cgacttgagt
      721 gataaattta acgcatacct ggcaaacaag tgggtattca ttactattac caatgatcgc
      781 ctgagtagtg ccaacctgta catcaacggc gtattgatgg gttccgcaga aattaccggc
      841 ttgggtgcca tccgtgaaga taacaatatc actttgaaac tcgaccgctg caacaataat
      901 aaccaatatg tatcgatcga taaattccgg atcttttgta aagctcttaa cccgaaagag
      961 attgagaaac tctacacgtc atatttgtcc attaccttcc tgcgtgattt ctggggcaat
     1021 ccgctgcggt acgacaccga atactacctt attcctgtcg cctatagtag taaagatgtc
     1081 cagttaaaaa acattacaga ttatatgtac cttaccaacg cgccttcgta caccaacggg
     1141 aagctgaaca tttattaccg ccgtctgtac agcggtctga aatttatcat taaacggtat
     1201 acgcctaaca atgagattga ctccttcgtc cggtcggggg actttatcaa attgtatgtt
     1261 tcatataata acaacgaaca cattgtcggc tacccaaaag atggaaacgc gtttaataac
     1321 ctcgaccgca ttctgcgtgt cggctacaac gcgccaggaa tcccgttata caagaaaatg
     1381 gaagccgtca aactccgcga tcttaaaact tattcagtgc agcttaaact ctacgatgac
     1441 aaagatgctt cactgggttt agtcggaact cataacggcc agatcggcaa cgatccaaac
     1501 cgcgacatct taatcgcgtc gaactggtat tttaaccacc tgaaagataa aaccctgacc
     1561 tgtgattggt atttcgtccc taccgatgaa ggctggacca atgatactag tagcggccgc
     1621 tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
     1681 agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1741 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1801 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1861 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
     1921 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1981 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2041 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2101 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2161 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2221 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2281 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2341 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2401 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2461 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2521 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2581 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg
     2641 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     2701 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
     2761 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     2821 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     2881 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     2941 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     3001 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     3061 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     3121 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     3181 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     3241 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     3301 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     3361 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     3421 cat
//
