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Revision as of 09:28, 18 August 2015

Classic parallax page

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Home
kenneth

Kenneth Lim Kun Ming

Research Interest: Bioinformatics, Genetic analysis Random fact: Is apparently Schrˆdinger's Biologist

Clarice Hong Kit Yee

Research Interest: genetics, RNA, cancer Past research projects: (can't rmb, tell you later)

Current project: Differential roles of SALL4A and SALL4B in HCC

chiyan

Wong Chi Yan

Research Interest: Microbiology, molecular biology, proteomics

Past research project: Genetic studies on Salmonella biofilms

Current project: Role of fumarase and cysteine dehydrogenase in DNA damage response

Random fact: Likes statistics and playing volleyball :)

Yeo Xin Yi

Research Interests: Neurobiology, Neurosciences

Past research projects: Role of STAT in neuroinflammation and the pathogenesis of Alzheimer's Disease

Current research projects: Synaptic plasticity threshold in hippocampal CA1 pyramidal neurons, Role of WNK1 in neuronal survival and development

Random fact: Blah ~

Tan Yi Han

Research Interests: Pathogens, Immunology, Synthetic biology

Past research projects: Genetic studies on plant pathogenic fungi, Drug screening for Acute Lymphoblastic Leukemia

Current project: Characterisation of Klebsiella pneumoniae isolates from liver abscess

Random fact: Knits and bakes in spare time. =)

yanting Hee Yanting

Research Interests: RNA, genomics, epigenetics

Past research projects: microRNA as a potential therapeutic strategy for colorectal cancer, Characterising LPA1 antagonists using calcium imaging

Current project: The role and targeting of EZH2 in lymphoma

Random fact: Plays the erhu and self-learning cello and classical guitar

Adrian Tan Hong Ji

Research Interest: Genetic Engineering, Cancer, Immunology

Random fact: 500 Hours in Terraria

yunting

Soong Yun Ting

Research Interests: Proteomics, Past research projects: Genetic studies on Salmonella biofilms

Current project: Identification of protein players in metastasis

Random fact: Plays the harmonica

duy

Nguyen Duy

Research Interest: Pharmacoinfomatics, Bioinformatics, Neurosciences

Past research project: Genetic linkage analysis of asthma

Current project: Neurodegeneration of Drosophila Melanogaster.

Random fact: 0 hour in Terraria

ourBelovedMentors
linda_stuti_leslie

Leslie

Leslie Gapter

Dr. Leslie is trained as a molecular biologist and her dissertation focused on breast development and tumorigenesis. Leslie joined NUS in 2005 and her past research has focused on analyzing botanical products for breast and prostate cancer treatment.

In 2008, Leslie became a full time scientific writer at the Mechanobiology Institute, Singapore, before moving into her current position as a Lecturer in 2010. Leslie teaches 'The Cell' module, which examines the universal mechanics and functions of cells from an integrated science perspective, for the Special Program in Science.

Linda

Linda J Kenney

Dr Kenney is a Professor of Microbiology at the University of Illinois-Chicago. Her laboratory studies two-component systems in bacteria that control gene expression at a single cell and nanometer level.

Stuti

Stuti Desai

She joined the Kenney group in May, 2012 with a strong urge to amalgamate her doctoral training in studying silent genetic systems in enterics to decipher the behavior of bacteria under various environmental challenges. She obtained her doctorate from the Indian Institute of Science, Bangalore, India, under the guidance of Prof Subramony Mahadevan. She studied Biochemistry for my Master's degree and Chemistry, Physics and Zoology for her Bachelor's degree at the Maharaja Sayajirao University of Baroda, Baroda, India.

theProject

Description

For description

Experiment and Protocol

For experiment and protocol

Result

For result

Design

For design

Parts

Team part

For Team part

Basic part

For basic part

Composite part

For composite part

Part collection

For part collection

notebook

Yi Han

Received 2 bacterial stab cultures, EsaR/I plasmid from addgene (CHL) and BBa_K299812 from iGEM HQ.

Streaked out on plates with amp.

Adrian

Transformed:

+Kit plate 1 9N Ba_K763002 chl

+Kit plate 4 13L BBa_E0040 amp

DNa was received in powder form in plates, and resuspended in 10ul ultrapure H2O respectively. Plates were stored in -20/

Yi Han

Plates cracked in incubator as they dried from lack of humidity.

Transfer to small incubator with beaker of water for humidity no single colonies for inv plasmid -> streak again.

Wrong antibiotic for EsaR/I plasmid-> streak out again

Yi Han

Inoculate single colony of invasin plasmid carrying bacteria in 3mL LB+amp

Transformation of 13L repeated with 1ul of DNA.

Xin Yi

No colonies grew for 13L on all plates ->adjust incubator, make new media

Miniprep of inoculated bacteria for invasin plasmid

RE of invasin plasmid: RsaI 1ul, EcoRI 1ul, INv plasmid 7.5ul, H20 35.5ul, NEB buffer 4 5ul, 37degC for 1h 20min

RE control: RsaI 0ul, EcoRI 0ul, Inv plasmid 7.5ul, H2O 37.5ul, BUffer 4 5ul

29_05_gelRun

Repeat transformation of 13L with 1ul
human practice
safety
done