Difference between revisions of "Team:Toulouse/Experiments"

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<td><!-- TFB1 200mL -->
 
<td><!-- TFB1 200mL -->
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<ul style="font-size:15px;" align="right">
+
<ul style="font-size:15px;" align="left">
 
<li>  0.59g KOAc </li>
 
<li>  0.59g KOAc </li>
 
<li> 2.42g RbCl </li>
 
<li> 2.42g RbCl </li>

Revision as of 10:09, 1 September 2015

iGEM Toulouse 2015

Experiments & Protocols


Transformation Protocol: RbCl method

Cloning
YETM 500mL TFB1 200mL TFB2 200mL
  • 2.5g Yeast Extract
  • 10g Tryptone
  • 5g MgSO4.7H2O
  • Adjust pH to 7.5 with KOH
  • For Plates: add 7.5g of Agar
  • 0.59g KOAc
  • 2.42g RbCl
  • 0.29g CaCl2.2H2O
  • 1.98g MnCl2.4H2O
  • Adjust to pH 5.8 with 0.2 M acetic acid
  • Add dH2O to 200mL
  • Filter sterilize
  • Store refrigerated at 4°C
  • 2.5g Yeast Extract
  • 10g Tryptone
  • 5g MgSO4.7H2O
  • Adjust pH to 7.5 with KOH
  • For Plates: add 7.5g of Agar

References