Difference between revisions of "Team:KU Leuven/Research/Methods"
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<li><h4><a onclick="ShowHide('HiddenDiv5')">Chemocompetent cells</a></h4> | <li><h4><a onclick="ShowHide('HiddenDiv5')">Chemocompetent cells</a></h4> | ||
<div class="mid" id="HiddenDiv5" style="display: none;"> | <div class="mid" id="HiddenDiv5" style="display: none;"> | ||
− | <p> | + | <p style "margin = 17px"> |
Materials</p> | Materials</p> | ||
<ul> | <ul> | ||
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<li>8. Remove the supernatant, and resuspend the cell pellet in 6 ml 0.1 M CaCl2 solution plus 15% glycerol. </li> | <li>8. Remove the supernatant, and resuspend the cell pellet in 6 ml 0.1 M CaCl2 solution plus 15% glycerol. </li> | ||
<li>9. Pipet 0.4-0.5 ml of the cell suspension into sterile 1.5 ml micro-centrifuge tubes. </li> | <li>9. Pipet 0.4-0.5 ml of the cell suspension into sterile 1.5 ml micro-centrifuge tubes. </li> | ||
+ | </br> | ||
<li> Freeze these tubes on dry ice and then transfer them to -70 C freezer. </li> | <li> Freeze these tubes on dry ice and then transfer them to -70 C freezer. </li> | ||
</ul> | </ul> |
Revision as of 08:12, 3 September 2015