Difference between revisions of "Team:KU Leuven/Research/Methods"
Line 157: | Line 157: | ||
<ul> | <ul> | ||
− | <li> 1. Inoculate one colony from LB plate into 2 ml LB liquid medium. Shake at 37 °C overnight. </li> | + | <li style = "text-align: left; list-style-type: none;> 1. Inoculate one colony from LB plate into 2 ml LB liquid medium. Shake at 37 °C overnight. </li> |
<li> 2. Inoculate 1-ml overnight cell culture into 100 ml LB medium (in a 500 ml flask). Shake vigorously at 37 °C to OD600 ~ 0.25-0.3 (usually it takes about 1.5-hours). <li> | <li> 2. Inoculate 1-ml overnight cell culture into 100 ml LB medium (in a 500 ml flask). Shake vigorously at 37 °C to OD600 ~ 0.25-0.3 (usually it takes about 1.5-hours). <li> | ||
<li>3. Chill the culture on ice for 15 min. Also make sure the 0.1M CaCl2 solution and 0.1M CaCl2 plus 15% glycerol are on ice </li> | <li>3. Chill the culture on ice for 15 min. Also make sure the 0.1M CaCl2 solution and 0.1M CaCl2 plus 15% glycerol are on ice </li> | ||
Line 175: | Line 175: | ||
<div class="mid" id="HiddenDiv6" style="display: none;"> | <div class="mid" id="HiddenDiv6" style="display: none;"> | ||
</br> | </br> | ||
− | <p style = "margin : 17px"> | + | <p style = "margin : 17px"> Materials </p> |
<p> | <p> | ||
<ul> | <ul> |
Revision as of 08:15, 3 September 2015