Difference between revisions of "Team:Toulouse/Modeling"

As said before, the aim of our project is to create a biological system able to produce two molecules: butyric acid and formic acid.
To achieve this, we need to modify the existing balance between the metabolic pathways present in E.Coli. Indeed, we want to optimize butyrate and formate productions in our bacterium by adjusting environmental conditions in order to obtain the desired concentrations of the associated acids. Below are represented all metabolic pathways and metabolites known to date in Escherichia coli K12 MG1655 (most known model). It was obtained from the KEGG database [1].
The first part of this work is to determine the metabolic pathways in which both molecules of interest are taking part, in order to well immerse ourselves in their roles and effects.

Formate is already produced by E.coli, in our project we need Apicoli to produce more. To optimize biosynthesis we had to study all the genes coding for enzymes involved in this pathway. We focused ourselves on the Pyruvate Formate Lyase (PFL), this enzyme provoke the degradation of pyruvate producing formate.

The subnetwork presented below is the result of this mapping, it was realised with the MetExplore platform [2] and present all known enzymes from the Kegg and ByoCyc databases involved with the production or consumption of formate. This map will help us predict the consequences of formate overproduction in Apicoli. Formate, harmful if to much is produced, is metabolized into others products most of the time. We thus have to find the balance between producing enough formate without killing Apicoli.

To help ourselves creating Apicoli we modelised our system by using Flux Balance Analysis (FBA) and Flux Variability Analysis (FVA). We used the most recent described model for E.coli K12 MG1655, describing all metabolic pathways known and up to date. This XML file