Difference between revisions of "Team:Amoy/Notebook"
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| − | <p style="font-size: 18px | + | <p style="font-size: 18px;"><strong>Purpose: </strong>Extract plasmid from dry powder</br> |
<strong>Steps:</strong></br> | <strong>Steps:</strong></br> | ||
1. Add 20ul ddH20 to solve the dry powder</br> | 1. Add 20ul ddH20 to solve the dry powder</br> | ||
2. Suck 10ul of plasmid into 50ul of competent cell for transformation</br></p> | 2. Suck 10ul of plasmid into 50ul of competent cell for transformation</br></p> | ||
| − | <img | + | <img style="width: 30%; margin-right: 70%;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" /> |
| − | + | ||
| − | <p style="font-size: 18px | + | <p style="font-size: 18px;">3. Pick a single colony from the agar plate using a sterile pipette tip. </br> |
4. Culture at 10ml LB of chloramphenicol for 12h</br> | 4. Culture at 10ml LB of chloramphenicol for 12h</br> | ||
5. Plasmid minipre</br></br> | 5. Plasmid minipre</br></br> | ||
Revision as of 12:07, 10 September 2015
NOTEBOOK
Initially, they used isolated enzymes, which can be disadvantageous for the reason that enzymes are always destabilized in the isolation and purification process. What's more, the cofactor-NADH is rather an expensive raw material, which will enhance the cost of L-tert-leucine production. So scientists introduced whole-cell biocatalysts to L-tert-leucine production. Whole-cell biocatalysts could stabilize enzymes and reduce the addition level of cofactor NADH. In the path of building our biobricks, we divided the circuits into two modules. One is promoter linked with rbs and the other is gene linked with terminator. The dendrogram below is our experiments detail. Click each bottom for more information.
CONTACT US
Email: igemxmu@gmail.com
Website: 2015.igem.org/Team:Amoy
Address: Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005
