Difference between revisions of "Team:Amoy/Notebook"

Line 75: Line 75:
 
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_h1">Steps:</br></p>
−
<p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br>
+
<p class="detail_p">1. Add 20μl ddH<sub>2</sub>O to solve the dry powder</br>
−
2. Suck 10ul of plasmid into 50ul of competent cell for transformation</br></p>
+
2. Suck 10μl of plasmid into 50μl of competent cell for transformation</br></p>
 
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
 
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
−
4. Culture at 10ml LB of chloramphenicol for 12h</br>
+
4. Culture at 10ml LB of chloramphenicol for 12h, 37℃, 200rpm</br>
−
5. Plasmid minipre</br></p>
+
5. Plasmid Extraction</br></p>
−
<p class="detail_h1">Product:</br></p>
+
<p class="detail_h1">Product: </br></p>
−
<p class="detail_p">plasmid of promoter J23100</br></p>
+
<p class="detail_p"> Plasmid of promote_J23100</br></p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 93: Line 93:
 
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_h1">Steps:</br></p>
−
<p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br>
+
<p class="detail_p">1. Add 20μl ddH20 to solve the dry powder</br>
−
2. Suck 10ul of plasmid into 50ul of competent cell for transformation</br></p>
+
2. Suck 10μl of plasmid into 50μl of competent cell for transformation</br></p>
 
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
 
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
−
4. Culture at 10ml LB of ampicillin for 12h</br>
+
4. Culture at 10ml LB of ampicillin for 12h, 37℃, 200rpm</br>
−
5. Plasmid minipre</br></p>
+
5. Plasmid Extraction</br></p>
 
<p class="detail_h1">Product:</br></p>
 
<p class="detail_h1">Product:</br></p>
−
<p class="detail_p">plasmid of promoter LacI</br></p>
+
<p class="detail_p">Plasmid of promoter_LacI</br></p>
 
</div>
 
</div>
 
</div>
 
</div>
Line 111: Line 111:
 
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_p">Extract plasmid from dry powder</br></p>
 
<p class="detail_h1">Steps:</br></p>
 
<p class="detail_h1">Steps:</br></p>
−
<p class="detail_p">1. Add 20ul ddH20 to solve the dry powder</br>
+
<p class="detail_p">1. Add 20μl ddH20 to solve the dry powder</br>
−
2. Suck 10ul of plasmid into 50ul of competent cell for transformation</br></p>
+
2. Suck 10μl of plasmid into 50μl of competent cell for transformation</br></p>
 
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
 
<img style="width: 30%; margin-right: 70%; margin-top: 10px; margin-bottom: 0px;" src="https://static.igem.org/mediawiki/2015/8/87/Amoy-Notebook_Node41_figure1.jpg" />
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
 
<p class="detail_p"></br>3. Pick a single colony from the agar plate using a sterile pipette tip. </br>
−
4. Culture at 10ml LB of ampicillin for 12h</br>
+
4. Culture at 10ml LB of ampicillin for 12h, 37℃, 200rpm</br>
−
5. Plasmid minipre</br></p>
+
5. Plasmid Extraction</br></p>
 
<p class="detail_h1">Product:</br></p>
 
<p class="detail_h1">Product:</br></p>
−
<p class="detail_p"> </br></p>
+
<p class="detail_p"> Plasmid of RBS_B0034</br></p>
 
</div>
 
</div>
 
</div>
 
</div>

Revision as of 07:13, 12 September 2015

Aomy/Project

NOTEBOOK

Initially, they used isolated enzymes, which can be disadvantageous for the reason that enzymes are always destabilized in the isolation and purification process. What's more, the cofactor-NADH is rather an expensive raw material, which will enhance the cost of L-tert-leucine production. So scientists introduced whole-cell biocatalysts to L-tert-leucine production. Whole-cell biocatalysts could stabilize enzymes and reduce the addition level of cofactor NADH.

In the path of building our biobricks, we divided the circuits into two modules. One is promoter linked with rbs and the other is gene linked with terminator. The dendrogram below is our experiments detail. Click each bottom for more information.

CONTACT US

Email: igemxmu@gmail.com

Website: 2015.igem.org/Team:Amoy

Address: Xiamen University, No. 422, Siming South Road, Xiamen, Fujian, P.R.China 361005