Difference between revisions of "Team:NCTU Formosa/Notebook"

Revision as of 08:06, 14 September 2015

Lab note

2015 March
2015 April
2015 May
2015 June
2015 July
2015 August
2015 September

Sun.	Mon.	Tue.	Wed.	Thu.	Fri.	Set.
			1	2	3	4
5	6	7	8	9	10	11
12	13	14	15 Transformation of GBP[FadL-GBP] Cultivation of OmpA[OmpA+pSB1K3] K^r	16 Cultivation of GBP[FadL-GBP] Plasmid purification of OmpA[OmpA+pSB1K3] K^r Digestion of OmpA[OmpA+pSB1K3] K^r (EcoRI and PstI)	17 Plasmid purification of GBP[FadL-GBP] Digestion of GBP[FadL-GBP] (EcoRI and PstI) Electrophoresis of digested product: GBP[FadL-GBP] (EcoRI and PstI)	18 Transformation of ligated product: GBP[FadL-GBP+pSB1C3] C^r
19 Cultivation of ligated product: GBP[FadL-GBP] C^r PCR product: Terminator[J61048]	20	21 Cultivation of GFP[J23101+B0034+E0040+J61048+pSB1A3] A^r OmpA[OmpA+pSB1C3] C^r GBP[FadL-GBP+pSB1C3] C^r	22 Plasmid purification of GFP[J23101+B0034+E0040+J61048+pSB1A3] A^r OmpA[OmpA+pSB1C3] C^r GBP[FadL-GBP+PSB1C3] C^r Digestion of GFP[J23101+B0034+E0040+J61048] A^r (EcoRI and PstI) OmpA[OmpA] C^r (EcoRI and PstI) GBP[FadL-GBP] C^r (EcoRI and PstI) Electrophoresis of digested product: GFP[J23101+B0034+E0040+J61048] A^r (EcoRI and PstI) OmpA[OmpA] C^r (EcoRI and PstI) GBP[FadL-GBP] C^r (EcoRI and PstI)	23 Digestion of OmpA[OmpA] C^r (EcoRI and SpeI) GBP[FadL-GBP] C^r (EcoRI and SpeI) Electrophoresis of digested product: OmpA[OmpA] C^r (EcoRI and SpeI) GBP[FadL-GBP] C^r (EcoRI and SpeI)	24 Digestion of OmpA[OmpA] C^r (XbaI and PstI) GBP[FadL-GBP] C^r (XbaI and PstI)	25 Electrophoresis of digested product: OmpA[OmpA] C^r (XbaI and PstI) GBP[FadL-GBP] C^r (XbaI and PstI) Digestion of RBS[B0034] A^r (XbaI and PstI) backbone[pSB1A3] A^r (EcoRI and PstI) Terminator[J61048] A^r (XbaI and PstI) Electrophoresis of digested product: RBS[B0034] A^r (XbaI and PstI) backbone[pSB1A3] A^r (EcoRI and PstI) Terminator[J61048] A^r (XbaI and PstI) Ligation of OmpA[J23101+B0034+OmpA+pSB1A3] A^r GBP[GBP+B0034+pSB1K3] K^r OmpA[OmpA+J61048+pSB1K3] K^r
26 Ligation of RFP[E1010+B0034+pSB1K3] K^r Transformation of ligated product: OmpA[J23101+B0034+OmpA+pSB1A3] A^r GBP[FadL-GBP+B0034+pSB1K3] K^r OmpA[OmpA+J61048+pSB1K3] K^r Electrophoresis of digested product: RFP[E1010+J61048+pSB1K3] K^r [J23101+B0034]	27 Digestion of Terminator[J61048] A^r (XbaI and PstI) PCR of ligated product: OmpA[J23101+B0034+OmpA+pSB1A3] A^r OmpA[OmpA+J61048+pSB1K3] K^r	28 Electrophoresis of RFP[E1010+J61048+pSB1K3] K^r OmpA[J23101+B0034+OmpA+pSB1A3] A^r Transformation of ligated product: GBP[B0034+FadL-GBP+pSB1A3] A^r OmpA[OmpA+J61048+pSB1K3] K^r PCR of ligated product OmpA[J23101+B0034+OmpA] A^r	29 Cultivation of RFP[E1010+J61048+pSB1K3] K^r PCR of OmpA[J23101+B0034+OmpA] A^r Electrophoresis of OmpA[J23101+B0034+OmpA] A^r Ligation of GBP[FadL-GBP+J61048+pSB1K3] K^r	30 Plasmid purification of RFP[E1010+J61048+pSB1K3] K^r Ligation of OmpA[J23101+B0034+OmpA] A^r PCR of OmpA[J23101+B0034+OmpA] A^r Transformation of mini: Terminator[J61048] ligated product: OmpA[J23101+B0034+OmpA] A^r

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NCTU_Formosa APOllO

National Chaio Tung University
Engineering Building 6 EF455, 1001 University Road, Hsinchu 300, Taiwan, ROC.

@@ Line 539: / Line 539: @@
 						<td >
                              <B>15</B><br><br>
-							Transformation of <br>GBP[FadL-GBP]<br>
+							<b>Transformation of</b><br>GBP[FadL-GBP]<br>
 							Cultivation of <br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
                          </td>
@@ Line 546: / Line 546: @@
 							Cultivation of <br>GBP[FadL-GBP]
 <br>
-Plasmid purification of <br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
+<b>Plasmid purification of</b><br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
 <br>
-Digestion of <br>OmpA[OmpA+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+<b>Digestion of</b><br>OmpA[OmpA+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
                          </td>
                          <td>
-                             <B>17</B><br><br>Plasmid purification of
+                             <B>17</B><br><br><b>Plasmid purification of</b>
 <br>GBP[FadL-GBP]
 <br>
-Digestion of
+<b>Digestion of</b>
 <br>GBP[FadL-GBP]  (EcoRI and PstI)
 <br>
-Electrophoresis of digested product:
+<b>Electrophoresis of</b><br>digested product:
 <br>GBP[FadL-GBP]  (EcoRI and PstI)
@@ Line 564: / Line 564: @@
 						<td >
                             <B>18</B><br><br>
-						   Transformation of ligated product:
+						   <b>Transformation of</b><br>ligated product:
 <br>GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
@@ Line 572: / Line 572: @@
                          <td >
                              <B>19</B><br><br>
-							Cultivation of
+							<b>Cultivation of</b>
 <br>ligated product: GBP[FadL-GBP] C<sup>r</sup>
 <br>PCR product: Terminator[J61048]
@@ Line 582: / Line 582: @@
                          <td>
                              <B>21</B><br><br>
-							Cultivation of
+							<b>Cultivation of</b>
 <br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 <br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
@@ Line 590: / Line 590: @@
 						<td >
                              <B>22</B><br><br>
-							Plasmid purification of
+							<b>Plasmid purification of</b>
 <br>GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
 <br>OmpA[OmpA+pSB1C3] C<sup>r</sup>
 <br>GBP[FadL-GBP+PSB1C3] C<sup>r</sup>
 <br>
-Digestion of
+<b>Digestion of</b>
 <br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 <br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
 <br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and PstI)
 <br>
-Electrophoresis of digested product:
+<b>Electrophoresis of</b><br>digested product:
 <br>GFP[J23101+B0034+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
 <br>OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
@@ Line 608: / Line 608: @@
                          <td>
                              <B>23</B><br><br>
-							Digestion of
+							<b>Digestion of</b>
 OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 <br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
 <br>
-Electrophoresis of digested product:
+<b>Electrophoresis of</b><br>digested product:
 <br>OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
 <br>GBP[FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
@@ Line 619: / Line 619: @@
                          <td>
                              <B>24</B><br><br>
-							Digestion of
+							<b>Digestion of</b>
 <br>OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 <br>GBP[FadL-GBP] C<sup>r</sup> (XbaI and PstI)
@@ Line 627: / Line 627: @@
 						<td >
                              <B>25</B><br><br>
-							Electrophoresis of digested product:
+							<b>Electrophoresis of</b><br>digested product:
 <br>OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
 <br>GBP[FadL-GBP] C<sup>r</sup> (XbaI and PstI)
 <br>
-Digestion of
+<b>Digestion of</b>
 <br>RBS[B0034] A<sup>r</sup> (XbaI and PstI)
 <br>backbone[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 <br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 <br>
-Electrophoresis of digested product:
+<b>Electrophoresis of</b><br>digested product:
 <br>RBS[B0034] A<sup>r</sup> (XbaI and PstI)
 <br>backbone[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
 <br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 <br>
-Ligation of
+<b>Ligation of</b>
 <br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 <br>GBP[GBP+B0034+pSB1K3] K<sup>r</sup>
@@ Line 651: / Line 651: @@
                          <td >
                              <B>26</B><br><br>
-							Ligation of
+							<b>Ligation of</b>
 <br>RFP[E1010+B0034+pSB1K3] K<sup>r</sup>
 <br>
-Transformation of ligated product:
+<b>Transformation</b><br>of ligated product:
 <br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 <br>GBP[FadL-GBP+B0034+pSB1K3] K<sup>r</sup>
 <br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
 <br>
-Electrophoresis of digested product:
+<b>Electrophoresis of</b><br>digested product:
 <br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 <br>[J23101+B0034]
@@ Line 666: / Line 666: @@
                          <td>
                             <B>27</B><br><br>
-						   Digestion of
+						   <b>Digestion of</b>
 <br>Terminator[J61048] A<sup>r</sup> (XbaI and PstI)
 <br>
-PCR of ligated product:
+<b>PCR of</b><br>ligated product:
 <br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
 <br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
@@ Line 676: / Line 676: @@
                          <td>
                              <B>28</B><br><br>
-							Electrophoresis of
+							<b>Electrophoresis of</b>
 <br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
 <br>OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
-Transformation of ligated product:
+<b>Transformation of</b><br>ligated product:
 <br>GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
 <br>OmpA[OmpA+J61048+pSB1K3] K<sup>r</sup>
-PCR of ligated product
+<b>PCR of</b><br>ligated product
 <br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
@@ Line 690: / Line 690: @@
 						<td >
                              <B>29</B><br><br>
-							Cultivation of
+							<b>Cultivation of</b>
 <br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
-PCR of
+<b>PCR of</b>
 <br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
-<br>Electrophoresis of
+<br><b>Electrophoresis of</b>
 <br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
-Ligation of
+<b>Ligation of</b>
 <br>GBP[FadL-GBP+J61048+pSB1K3] K<sup>r</sup>
@@ Line 706: / Line 706: @@
                          <td>
                              <B>30</B><br><br>
-						   Plasmid purification of
+						   <b>Plasmid purification of</b>
 <br>RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
-Ligation of
+<b>Ligation of</b>
 <br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
-PCR of
+<b>PCR of</b>
 <br>OmpA[J23101+B0034+OmpA] A<sup>r</sup>
-Transformation of
+<b>Transformation of</b>
 <br>mini: Terminator[J61048]
 <br>ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>