Difference between revisions of "Team:NCTU Formosa/Notebook"

Revision as of 08:35, 14 September 2015

Lab note

2015 March
2015 April
2015 May
2015 June
2015 July
2015 August
2015 September

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			1	2	3	4
5	6	7	8	9	10	11
12	13	14	15 Transformation of GBP[FadL-GBP] Cultivation of OmpA[OmpA+pSB1K3] K^r	16 Cultivation of GBP[FadL-GBP] Plasmid purification of OmpA[OmpA+pSB1K3] K^r Digestion of OmpA[OmpA+pSB1K3] K^r (EcoRI and PstI)	17 Plasmid purification of GBP[FadL-GBP] Digestion of GBP[FadL-GBP] (EcoRI and PstI) Electrophoresis of digested product: GBP[FadL-GBP] (EcoRI and PstI)	18 Transformation of ligated product: GBP[FadL-GBP+pSB1C3] C^r
19 Cultivation of ligated product: GBP[FadL-GBP] C^r PCR product: Terminator[J61048]	20	21 Cultivation of GFP[J23101+B0034+E0040+J61048+pSB1A3] A^r OmpA[OmpA+pSB1C3] C^r GBP[FadL-GBP+pSB1C3] C^r	22 Plasmid purification of GFP[J23101+B0034+E0040+J61048+pSB1A3] A^r OmpA[OmpA+pSB1C3] C^r GBP[FadL-GBP+PSB1C3] C^r Digestion of GFP[J23101+B0034+E0040+J61048] A^r (EcoRI and PstI) OmpA[OmpA] C^r (EcoRI and PstI) GBP[FadL-GBP] C^r (EcoRI and PstI) Electrophoresis of digested product: GFP[J23101+B0034+E0040+J61048] A^r (EcoRI and PstI) OmpA[OmpA] C^r (EcoRI and PstI) GBP[FadL-GBP] C^r (EcoRI and PstI)	23 Digestion of OmpA[OmpA] C^r (EcoRI and SpeI) GBP[FadL-GBP] C^r (EcoRI and SpeI) Electrophoresis of digested product: OmpA[OmpA] C^r (EcoRI and SpeI) GBP[FadL-GBP] C^r (EcoRI and SpeI)	24 Digestion of OmpA[OmpA] C^r (XbaI and PstI) GBP[FadL-GBP] C^r (XbaI and PstI)	25 Electrophoresis of digested product: OmpA[OmpA] C^r (XbaI and PstI) GBP[FadL-GBP] C^r (XbaI and PstI) Digestion of RBS[B0034] A^r (XbaI and PstI) backbone[pSB1A3] A^r (EcoRI and PstI) Terminator[J61048] A^r (XbaI and PstI) Electrophoresis of digested product: RBS[B0034] A^r (XbaI and PstI) backbone[pSB1A3] A^r (EcoRI and PstI) Terminator[J61048] A^r (XbaI and PstI) Ligation of OmpA[J23101+B0034+OmpA+pSB1A3] A^r GBP[GBP+B0034+pSB1K3] K^r OmpA[OmpA+J61048+pSB1K3] K^r
26 Ligation of RFP[E1010+B0034+pSB1K3] K^r Transformation of ligated product: OmpA[J23101+B0034+OmpA+pSB1A3] A^r GBP[FadL-GBP+B0034+pSB1K3] K^r OmpA[OmpA+J61048+pSB1K3] K^r Electrophoresis of digested product: RFP[E1010+J61048+pSB1K3] K^r [J23101+B0034]	27 Digestion of Terminator[J61048] A^r (XbaI and PstI) PCR of ligated product: OmpA[J23101+B0034+OmpA+pSB1A3] A^r OmpA[OmpA+J61048+pSB1K3] K^r	28 Electrophoresis of RFP[E1010+J61048+pSB1K3] K^r OmpA[J23101+B0034+OmpA+pSB1A3] A^r Transformation of ligated product: GBP[B0034+FadL-GBP+pSB1A3] A^r OmpA[OmpA+J61048+pSB1K3] K^r PCR of ligated product OmpA[J23101+B0034+OmpA] A^r	29 Cultivation of RFP[E1010+J61048+pSB1K3] K^r PCR of OmpA[J23101+B0034+OmpA] A^r Electrophoresis of OmpA[J23101+B0034+OmpA] A^r Ligation of GBP[FadL-GBP+J61048+pSB1K3] K^r	30 Plasmid purification of RFP[E1010+J61048+pSB1K3] K^r Ligation of OmpA[J23101+B0034+OmpA] A^r PCR of OmpA[J23101+B0034+OmpA] A^r Transformation of mini: Terminator[J61048] ligated product: OmpA[J23101+B0034+OmpA] A^r

Sun.	Mon.	Tue.	Wed.	Thu.	Fri.	Set.
					1 PCR of Transformation product: mini: Terminator[J61048] ligated product: OmpA[J23101+B0034+OmpA] A^r GBP[B0034+FadL-GBP+pSB1A3] A^r Electrophoresis of mini: Terminator[J61048] ligated product: OmpA[J23101+B0034+OmpA] A^r	2
3 Ligation of GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r	4 Ligation of GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r 5/4 Transformation off ligated products: GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r Digestion of RFP[E1010+J61048] (K^r) (XbaI and PstI) PCR of ligated products: GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r Electrophoresis of PCR products: GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r	5 Electrophoresis of digested products: RFP[E1010+J61048] (K^r) (XbaI and PstI) OmpA[J23101+B0034+OmpA] A^r (EcoRI and PstI) GBP[FadL-GBP+J61048] K^r (EcoRI and PstI) PCR of OmpA[J23101+B0034+OmpA+pSB1A3] A^r Cultivation of PCR product: RFP[E1010+J61048+pSB1K3] K^r GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r	6 Plasmid purification of RFP[E1010+J61048+pSB1K3] K^r GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r Digestion of GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r (EcoRI and PstI) Cultivation of PCR product: OmpA[J23101+B0034+OmpA+pSB1A3] A^r PCR of backbone: [pSB1C3] C^r [pSB1A3] A^r [pSB1K3] K^r Electrophoresis of PCR products: OmpA[J23101+B0034+OmpA+pSB1A3] A^r digested products: GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r (EcoRI and PstI)	7 Digestion of RFP[E1010+J61048+pSB1K3] K^r (EcoRI and PstI) RFP[E1010+J61048+pSB1K3] K^r (XbaI and PstI) [pSB1C3] C^r (EcoRI and PstI) [pSB1A3] A^r (EcoRI and PstI) [pSB1K3] K^r (EcoRI and PstI) OmpA[J23101+B0034+OmpA+pSB1A3] A^r (EcoRI and PstI) Plasmid purification of OmpA[J23101+B0034+OmpA+pSB1A3] A^r [pSB1K3] K^r Electrophoresis of digested products: RFP[E1010+J61048+pSB1K3] K^r (EcoRI and PstI) ???? RFP[E1010+J61048+pSB1K3] K^r (XbaI and PstI) [pSB1C3] C^r (EcoRI and PstI) [pSB1A3] A^r (EcoRI and PstI) [pSB1K3] K^r (EcoRI and PstI) Ligation of RFP[B0034+E1010+J61048+pSB1C3] C^r	8 Electrophoresis of digested products: OmpA[J23101+B0034+OmpA] A^r (EcoRI and PstI) Transformation off ligated products: RFP[B0034+E1010+J61048+pSB1C3] C^r PCR of GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r Electrophoresis of PCR products: GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r Transformation off GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r [B0030] A^r [J23110] A^r	9 PCR of GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r [B0030] A^r [J23110] A^r Electrophoresis of PCR products: GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r [B0030] A^r [J23110] A^r Cultivation of [B0030] A^r [J23110] A^r Ligation of GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r
10 Electrophoresis of [B0030] A^r Cultivation of [B0030] A^r Plasmid purification of [J23110] A^r [B0030] A^r GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r Digestion of [B0030] A^r (EcoRI and SpeI) [J23110] A^r (EcoRI and SpeI) GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r (EcoRI and PstI) GBP[B0034+FadL-GBP+pSB1A3] A^r (XbaI and PstI) Electrophoresis of digested products: [B0030] A^r (EcoRI and SpeI) GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r (EcoRI and PstI) GBP[B0034+FadL-GBP+pSB1A3] A^r (XbaI and PstI)	11 Electrophoresis of digested products: [J23110] A^r (EcoRI and SpeI) Ligation of RFP[B0030+E1010+J61048+pSB1C3] C^r GFP[B0030+E0040+J61048+pSB1K3] K^r Transformation off RFP[B0030+E1010+J61048+pSB1C3] C^r GFP[B0030+E0040+J61048+pSB1K3] K^r PCR of RFP[B0030+E1010+J61048+pSB1C3] C^r GFP[B0030+E0040+J61048+pSB1K3] K^r	12 PCR of ligated products: RFP[B0030+E1010+J61048+pSB1C3] C^r Cultivation of PCR products: RFP[B0030+E1010+J61048+pSB1C3] C^r Plasmid purification of RFP[B0030+E1010+J61048+pSB1C3] C^r Digestion of RFP[B0030+E1010+J61048] C^r (EcoRI and SpeI) RFP[B0030+E1010+J61048] C^r (EcoRI and PstI) Electrophoresis of digested products: RFP[B0030+E1010+J61048] C^r (EcoRI and SpeI) RFP[B0030+E1010+J61048] C^r (EcoRI and PstI) PCR products: RFP[B0030+E1010+J61048+pSB1C3] C^r	13 Ligation of [J23110+B0034+pSB1K3] K^r	14 Cultivation of OmpA[J23101+B0034+OmpA+pSB1A3] A^r Transformation off [J23110+B0034+pSB1K3] K^r Electrophoresis of digested products: GBP[J23101+B0034+FadL-GBP+pSB1A3] A^r (EcoRI and PstI) GBP[B0034+FadL-GBP+pSB1A3] A^r (XbaI and PstI) [pSB1K3] K^r (EcoRI and PstI)	15 Digestion of GBP[B0034+FadL-GBP+pSB1A3] A^r (EcoRI and PstI) Ligation of GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r	16 Transformation off GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r Electrophoresis of GBP[B0034+FadL-GBP+pSB1A3] A^r (EcoRI and PstI) GBP[B0034+FadL-GBP+pSB1A3] A^r (XbaI and PstI) Ligation of GBP[B0034+FadL-GBP+pSB1C3] C^r Transformation off [J23110+B0034+pSB1K3] K^r
17 Ligation of [J23110+B0034+pSB1K3] K^r GBP[J23110+B0034+FadL-GBP+pSB1K3] K^r PCR of GBP[B0034+FadL-GBP+pSB1C3] C^r GBP[J23110+B0034+FadL-GBP+pSB1K3] K^r Electrophoresis of PCR products: GBP[B0034+FadL-GBP+pSB1C3] C^r GBP[J23110+B0034+FadL-GBP+pSB1K3] K^r	18 Cultivation of GBP[B0034+FadL-GBP+pSB1C3] C^r Plasmid purification of GBP[B0034+FadL-GBP+pSB1C3] C^r Digestion of GBP[B0034+FadL-GBP] C^r (XbaI and PstI) RFP[B0030+E1010+J61048] C^r (XbaI and PstI) GFP[B0030+E0040+J61048] Transformation off [J23110+B0034+pSB1K3] K^r GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r GFP[B0030+E0040+J61048]	19 Ligation of GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r PCR of GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r Electrophoresis of digested products: RFP[B0030+E1010+J61048] C^r (XbaI and PstI) GBP[B0034+FadL-GBP] C^r (XbaI and PstI) PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r Transformation off scFv-B[scFv-B+pSB1K3] K^r scFv-C[scFv-C+pSB1K3] K^r GFP[B0030+E0040+J61048] Cultivation of GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r	20 Cultivation of OmpA[J23101+B0034+OmpA] A^r scFv-B[scFv-B+pSB1K3] K^r scFv-C[scFv-C+pSB1K3] K^r Plasmid purification of OmpA[J23101+B0034+OmpA] A^r GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r scFv-B[scFv-B+pSB1K3] K^r scFv-C[scFv-C+pSB1K3] K^r Digestion of OmpA[J23101+B0034+OmpA] A^r (EcoRI and Pst1) GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r (EcoRI and PstI) OmpA[J23101+B0034+OmpA] A^r (NcoI and PstI) scFv-B[scFv-B] A^r (EcoRI and PstI) scFv-C[scFv-C] A^r (EcoRI and PstI) Ligation of GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r Transformation off [J23110+B0034+pSB1K3] K^r [pSB3C5] 抗性 Electrophoresis of OmpA[J23101+B0034+OmpA] A^r (EcoRI and PstI)	21 Digestion of scFv-B[scFv-B] A^r (EcoRI and PstI) scFv-C[scFv-C] A^r (EcoRI and PstI) Electrophoresis of OmpA[J23101+B0034+OmpA] A^r (NcoI and PstI) scFv-B[scFv-B] A^r (EcoRI and PstI) scFv-C[scFv-C] A^r (EcoRI and PstI) Ligation of scFv-B[scFv-B+pSB1C3] C^r scFv-C[scFv-C+pSB1C3] C^r scFv-B[scFv-B+pSB1K3] K^r scFv-C[scFv-C+pSB1K3] K^r	22 Cultivation of [pSB3C5] C^r [J23110+B0034+pSB1K3] K^r Transformation off GBP[J23110+B0034+GBP] A^r PCR of scFv-B[scFv-B+pSB1C3] C^r scFv-C[scFv-C+pSB1C3] C^r scFv-B[scFv-B+pSB1K3] K^r scFv-C[scFv-C+pSB1K3] K^r Electrophoresis of scFv-B[scFv-B+pSB1C3] C^r scFv-C[scFv-C+pSB1C3] C^r scFv-B[scFv-B+pSB1K3] K^r scFv-C[scFv-C+pSB1K3] K^r Ligation of scFv-B[scFv-B+pSB1A3] A^r scFv-C[scFv-C+pSB1A3] A^r Transform of scFv-B[scFv-B+pSB1A3] A^r scFv-C[scFv-C+pSB1A3] A^r	23 Plasmid purification of [pSB3C5] C^r [J23110+B0034+pSB1K3] K^r Digestion of [pSB3C5] C^r (EcoRI and PstI) [J23110+B0034+pSB1K3] K^r (EcoRI and PstI) PCR of GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r Transformation off scFv-B[scFv-B+pSB1A3] A^r scFv-C[scFv-C+pSB1A3] A^r
24 Electrophoresis of digested products: [pSB3C5] C^r (EcoRI and PstI) [J23110+B0034+pSB1K3] K^r (EcoRI and PstI) Cultivation of PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r scFv-B[scFv-B+pSB1C3] C^r scFv-C[scFv-C+pSB1A3] A^r Plasmid purification of scFv-B[scFv-B+pSB1A3] A^r scFv-C[scFv-C+pSB1A3] A^r GFP[B0030+E0040+J61048] Digestion of [J23101+B0034] K^r (EcoRI and SpeI) Ligation of OmpA[J23101+B0034+OmpA+pSB3C5] C^r PCR of GBP[J23110+B0034+GBP] A^r by two pA^rts Electrophoresis of GBP[J23110+B0034+GBP] A^r	25 Transformation off OmpA[J23101+B0034+OmpA+pSB3C5] C^r Cultivation of GBP[J23110+B0034+FadL-GBP] A^r Digestion of GBP[B003+FadL-GBP] C^r (XbaI and PstI) scFv-B[scFv-B+pSB1C3] C^r (EcoRI and PstI) scFv-C[scFv-C+pSB1A3] A^r (EcoRI and PstI) GFP[B0030+E0040+J61048] (XbaI and PstI) Electrophoresis of GFP[B0030+E0040+J61048] scFv-B[scFv-B+pSB1C3] C^r (EcoRI and PstI) scFv-C[scFv-C+pSB1A3] A^r (EcoRI and PstI) GFP[B0030+E0040+J61048] (XbaI and PstI) Cultivation of scFv-B[scFv-B+pSB1C3] C^r (EcoRI and PstI) scFv-C[scFv-C+pSB1A3] A^r (EcoRI and PstI) GFP[B0030+E0040+J61048] (XbaI and PstI)	26 Ligation of OmpA[J23110+B0034+OmpA+pSB1A3] A^r GBP[J23110+B0034+GBP+pSB1A3] A^r Gel extraction of [J23110] A^r (SpeI and PstI) Plasmid purification of GBP[J23110+B0034+GBP] A^r by two pA^rts Digestion of GBP[J23110+B0034+GBP] A^r by two pA^rts (EcoRI and SpeI)	27 PCR of [J23101+B0034+OmpA+pSB3C5] Electrophoresis of GBP[J23110+B0034+GBP] A^r by two pA^rts (EcoRI and SpeI) GBP[B0034+FadL-GBP] C^r (XbaI and PstI) [J23110] A^r (SpeI and PstI)	28 Ligation of scFv-B[scFv-B+pSB1A3] A^r scFv-C[scFv-C+pSB1A3] A^r GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] 抗性?? scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A^r Transform of scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A^r GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] scFv-B[scFv-B+pSB1A3] A^r scFv-C[scFv-C+pSB1A3] A^r PCR of [J23101+B003+OmpA+pSB3C5] + primer(EX-OmpA and OmpA-S) OmpA[J23101+B0034+OmpA+pSB3C5] C^r Plasmid purification in gel slices of PCR products: OmpA[EX-OmpA-S] Gel extraction of OmpA[OmpA] (EcoRI and SpeI) Transformation off OmpA[J23101+B0034+OmpA+pSB3C5]	29 Plasmid purification of [J23110] A^r [pSB1C3] Digestion of [J23110] A^r (EcoRI and SpeI) [pSB1C3] (EcoRI and SpeI) Ligation of scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A^r [J23110+B0034+pSB1K3] K^r Electrophoresis of [J23110] A^r (EcoRI and SpeI) [pSB1C3] (EcoRI and SpeI) Transform of scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A^r [J23110+B0034+pSB1K3] K^r GFP[J23110+B0034+E0040+J61048+pSB1C3] C^r	30 PCR of scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A^r [J23110+B0034+pSB1K3] K^r GFP[J23110+B0034+E0040+J61048+pSB1C3] C^r
31 Cultivation of [J23110+B0034] K^r GFP[J23110+B0034+E0040+J61048+pSB1C3] C^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A^r scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A^r Plasmid purification of [J23110+B0034] K^r GFP[J23110+B0034+E0040+J61048+pSB1C3] C^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A^r scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A^r Digestion of [J23110+B0034] K^r (EcoRI and PstI) GFP[J23110+B0034+E0040+J61048+pSB1C3] C^r (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A^r (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A^r (EcoRI and PstI) [J23110+B0034] (EcoRI and SpeI) K^r [J23101+B0034] (EcoRI and SpeI) K^r OmpA[OmpA] C^r (EcoRI and PstI) [pSB1K3] (EcoRI and PstI) Electrophoresis of [J23110+B0034] K^r (EcoRI and PstI) GFP[J23110+B0034+E0040+J61048+pSB1C3] C^r (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A^r (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A^r (EcoRI and PstI) Ligation of OmpA[OmpA+pSB1C3] C^r

Sun.	Mon.	Tue.	Wed.	Thu.	Fri.	Set.
	1 Electrophoresis of [J23110+B0034] (EcoRI and SpeI) K^r [J23101+B0034] (EcoRI and SpeI) K^r OmpA[OmpA] (NcoI and PstI) GFP[K1489002+E0040] K^r Digestion of [pSB1C3] (EcoRI and PstI) [pSB1A3] (EcoRI and PstI) OmpA[OmpA] (EcoRI and NcoI) [pSB3C5] (EcoRI and PstI) Ligation of GFP[J23101+B0034+K1489002+E0040+pSB3C5] (C^r) GFP[J23110+B0034+K1489002+E0040+pSB3C5] (C^r) Electrophoresis of digested product: [pSB1C3] (EcoRI and PstI) [pSB1A3] (EcoRI and PstI) OmpA[OmpA] (EcoRI and NcoI) Cultivation of BFP[OmpA+K592100] PCR of GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r Electrophoresis of GBP[J23101+B0034+FadL-GBP+pSB1K3] K^r	2 PCR of OmpA[EX-OmpA-S+pSB1C3] C^r Electrophoresis of OmpA[EX-OmpA-S+pSB1C3] C^r OmpA[OmpA] (EcoRI and NcoI) Ligation of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E1010+J61048+pSB1K3] K^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K^r Digestion of [pSB1A3] (EcoRI and NcoI) Electrophoresis of digested products: [pSB1A3] (EcoRI and NcoI)	3 Ligation of OmpA[OmpA+psB1C3] C^r [J23110+B0034+pSB1K3] K^r Transform of OmpA[OmpA+psB1C3] C^r [J23110+B0034+pSB1K3] K^r Digestion of BFP[OmpA+ K592100] (NcoI and PstI) OmpA[OmpA] (NcoI and PstI)	4 Cultivation of [J23110+pSB1A3] A^r Transform of [pSB1C3] Digestion of [pSB1C3] (EcoRI and PstI) Ligation of OmpA[OmpA+pSB1C3] C^r OmpA[J23101+B0034+OmpA+pSB3C5] C^r Transform of [B0034+pSB1A3] A^r	5 Transform of OmpA[J23101+B0034+OmpA+pSB3C5] C^r Plasmid purification of [J23110+pSb1A3] A^r Digestion of [J23110+pSb1A3] A^r PCR of [J23110+pSb1A3] A^r BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r Transform of OmpA[OmpA+pSB1C3] C^r Electrophoresis of [pSB1C3] [B0034+pSB1A3] A^r BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r	6 Cultivation of [pSB1C3] Digestion of [pSB1C3] (EcoRI and SpeI)
7 Gel extraction of OmpA[EX-OmpA-S] 抗性 [pSB1C3] (EcoRI and SpeI) Gel extraction of [pSB1C3] (EcoRI and SpeI) Transform of GFP[J23101+B0034+E0040+J61048+pSB1A3] A^r [pSB1A3] OmpA[J23101+B0034+OmpA] A^r Ligation of OmpA[OmpA+pSB1C3] C^r Cultivation of [pSB1C3]	8 Transform of OmpA[OmpA+pSB1C3] C^r [pSB3K3] [pSB6A1] Plasmid purification of [pSB1C3] Digestion of OmpA[EX-OmpA-S] (EcoRI and SpeI) Electrophoresis of [pSB1C3] (EcoRI and SpeI) Transform of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K^r	9 Ligation of OmpA[OmpA+pSB1C3] C^r PCR of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K^r Cultivation of BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r Plasmid purification of [pSB3K3] [pSB6A1] Digestion of [pSB3K3] (EcoRI and PstI) [pSB6A1] (EcoRI and PstI)	10 Transform of OmpA[OmpA+pSB1C3] C^r Plasmid purification of [J23101+B0034+OmpA+K592100+pSB3C5] C^r Digestion of [J23101+B0034+OmpA+K592100] C^r (EcoRI and PstI) Electrophoresis of [pSB3K3] (EcoRI and PstI) [pSB6A1] (EcoRI and PstI) [pSB1A3] (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C] (EcoRI and PstI) Transform of [J23110+B0034] K^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Ligation of scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C^r Electrophoresis of [pSB3K3] BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r	11 Transform of scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C^r [J23110+B0034+pSB1C3] C^r Ligation of [J23110+B0034+pSB1K3] K^r [J23110+B0034+pSB1C3] C^r PCR of OmpA[OmpA+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C^r Electrophoresis of OmpA[OmpA+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C^r Cultivation of OmpA[OmpA+pSB1C3] C^r	12 PCR of [J23110+B0034+pSB1C3] C^r [B0034+pSB1A3] A^r Plasmid purification of OmpA[OmpA+pSB1C3] C^r Ligation of [J23110+B0034+pSB1C3] C^r Transform of [J23110+B0034+pSB1C3] C^r	13 Electrophoresis of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r (EcoRI and PstI)
14 SDS-page of [pSB1C3] scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r (EcoRI and PstI) [pSB3C5] scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C^r scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C^r scFv-B[OmpA+scFv-B] scFv-C[OmpA+scFv-C] Electrophoresis of [J23110] [B0034+FadL-GBP+B0030+E1010+J61048] 抗性 (XbaI and PstI) Ligation of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3K3] C^r GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] C^r Digestion of OmpA[OmpA+pSB1C3] C^r (EcoRI and SpeI) OmpA[OmpA+pSB1C3] C^r (EcoRI and PstI) Transform of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r Ligation of GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] A^r Transform of GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] A^r	15	16 Transform of [pSB3C5]	17 Cultivation of [pSB3C5]	18 Plasmid purification of [pSB3C5] Digestion of [pSB3C5]( EcoRI and PstI) Electrophoresis of [pSB3C5]( EcoRI and PstI) Ligation of BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r BFP[J23101+B0034+OmpA+K592100+pSB1C3] C^r	19 Transform of BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r BFP[J23101+B0034+OmpA+K592100+pSB1C3] C^r	20 PCR of BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r BFP[J23101+B0034+OmpA+K592100+pSB1C3] C^r Electrophoresis of BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r BFP[J23101+B0034+OmpA+K592100+pSB1C3] C^r
21	22	23 PCR of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K^r Cultivation of [pSB3C5] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r BFP[OmpA+K592100] 抗性 scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] [psB6A1] GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A^r [pSB1A3] GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C^r Ligation of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r	24 Plasmid purification of [pSB3C5]	25 Cultivation of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r Transform of [J23101+B0034+pSB1K3] K^r	26	27 Digestion of OmpA[OmpA] C^r (EcoRI and PstI) Electrophoresis of GBP[FadL-GBP] 抗性 (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A^r (EcoRI and PstI) OmpA[OmpA] C^r (EcoRI and PstI)
28 Cultivation of BFP[J23101+B0034+OmpA+K592100+pSB1A3] A^r GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] C^r GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r [pSB6A1] [pSB3C5] [pSB1C3] [pSB1A3] scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r Electrophoresis of [J23101+B0034] K^r GBP[FadL-GBP] 抗性 (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A^r (EcoRI and PstI) OmpA[OmpA] C^r (EcoRI and PstI) BFP[J23101+B0034+OmpA+K592100] A^r (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C^r (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] C^r (EcoRI and PstI) [pSB6A1] (EcoRI and PstI) OmpA[OmpA] C^r (XbaI and PstI) [E0040] A^r (XbaI and PstI) Ligation of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r [B0034+E0040+pSB1K3] K^r GBP[FadL-GBP+pSB6A1] A^r GBP[FadL-GBP+pSB1C3] C^r GBP[FadL-GBP+pSB3C5] C^r Digestion of BFP[J23101+B0034+OmpA+K592100] C^r (XbaI and PstI) SDS-page of [pSB1A3] [pSB1C3] BFP[J23101+B0034+OmpA+K592100+pSB1A3] A^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r [pSB3C5] [pSB6A1] GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] C^r GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A^r Transform of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r GBP[FadL-GBP+pSB1C3] C^r GBP[FadL-GBP+pSB3C5] C^r BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r	29 PCR of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r GBP[FadL-GBP+pSB1C3] C^r GBP[FadL-GBP+pSB3C5] C^r BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r Electrophoresis of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r	30 Electrophoresis of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r

Sun.	Mon.	Tue.	Wed.	Thu.	Fri.	Set.
			1 Cultivation of [pSB6A1] scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r Transform of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r Digestion of GBP[]FadL-GBP] 抗性 (EcoRI and PstI)	2 Digestion of [J23110] A^r (SpeI and PstI) Plasmid purification in gel slices of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r Digestion of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI) scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI) Electrophoresis of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI) scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI)	3 Gel extraction of [J23110] A^r (SpeI and PstI) [pSB6A1] (EcoRI and PstI) Transform of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r Electrophoresis of GBP[FadL-GBP] (EcoRI and Pstl) Ligation of GBP[FadL-GBP+pSB3C5] C^r GBP[FadL-GBP+pSB1C3] C^r GBP[FadL-GBP+pSB6A1] A^r	4 Transform of GBP[FadL-GBP+pSB3C5] C^r GBP[FadL-GBP+pSB1C3] C^r GBP[FadL-GBP+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r Electrophoresis of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r Cultivation of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r [J23101+B0034+pSB1K3] K^r
5 Plasmid purification of [J23101+B0034+pSB1K3] K^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r	6 Cultivation of [pSB1C3] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r [pSB6A1] scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r [pSB1A3] GFP[J23101+B0034+E0040+J61048+pSB1A3] A^r	7 Digestion of [J23101+B0034+pSB1K3] K^r (EcoRI and PstI) SDS-page of [pSB1C3] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r [pSB6A1] scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r [pSB1A3] GFP[J23101+B0034+E0040+J61048+pSB1A3] A^r Ligation of [J23110+B0034] A^r Electrophoresis of [J23101+B0034+pSB1K3] K^r (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r	8 Plasmid purification of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r Transform of [J23110+B0034] A^r BFP[J23101+B0034+OmpA+K592100] scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100] Electrophoresis of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI)	9 Electrophoresis of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+scFv-B+B0034+E0040+J61048+pSB1C3] C^r [pSB3C5] BFP[J23101+B0034+OmpA+scFv-B+B0030+e0040+J61048+pSB6A1] A^r Cultivation of scFv-B[J23110+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A^r	10 Plasmid purification of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r	11 PCR of [J23110+B0034] A^r Electrophoresis of [J23110+B0034] A^r
12 Cultivation of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r [J23110+B0034] A^r Plasmid purification of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r Digestion of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI)	13 Plasmid purification of [J23110+B0034] A^r Transform of [R0010] Electrophoresis of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI)	14 Digestion of [J23110+B0034] A^r (EcoRI and PstI) GBP[FadL-GBP] K^r (XbaI and PstI) OmpA[OmpA] C^r (EcoRI and SpeI) Electrophoresis of [J23110+B0034] A^r (EcoRI and PstI) GBP[FadL-GBP] K^r (XbaI and PstI) OmpA[OmpA] C^r (EcoRI and SpeI) Transform of [R0010] C^r Cultivation of [pSB1C3] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r	15 Digestion of [pSB6A1] [pSB3C5] Anti-Her 2[Herceptin+E1010] [J23101+B0034+OmpA+scFv-C] [J23101+B0034+OmpA+scFv-B] Cultivation of [pSB6A1] [R0010] PCR of OmpA[OmpA-linker] (EcoRI and PstI) Transform of [J23110+B0034] K^r BFP[K592100+J61048] K^r	16 Gel extraction of OmpA[OmpA-linker] (EcoRI and PstI) Ligation of OmpA[J23101+B0034+OmpA+pSB3C5] C^r BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r Digestion of OmpA[OmpA-linker] (EcoRI and PstI) Transform of OmpA[J23101+B0034+OmpA+pSB3C5] C^r	17 Electrophoresis of OmpA[OmpA-linker] (EcoRI and PstI) Anti-Her 2[Herceptin+E1010] (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI)	18 Ligation of OmpA[OmpA-linker+pSB1C3] C^r Anti-Her 2[Herceptin+E1010+pSB1C3] C^r
19 Transform of OmpA[OmpA-linker+pSB1C3] C^r Anti-Her 2[Herceptin+E1010+pSB1C3] C^r	20	21	22	23	24 Transform of OmpA[OmpA-linker+pSB1C3] C^r Anti-Her 2[Herceptin+E1010+pSB1C3] C^r PCR of OmpA[OmpA-linker+pSB1C3] C^r Anti-Her 2[Herceptin+E1010+pSB1C3] C^r Electrophoresis of OmpA[OmpA-linker+pSB1C3] C^r Anti-Her 2[Herceptin+E1010+pSB1C3] C^r	25 Cultivation of [R0010] C^r BFP[K592100+J61048] K^r PCR of OmpA[OmpA-linker+pSB1C3] C^r Anti-Her 2[Herceptin+E1010+pSB1C3] C^r Electrophoresis of OmpA[OmpA-linker+pSB1C3] C^r Anti-Her 2[Herceptin+E1010+pSB1C3] C^r
26 Plasmid purification of [R0010] C^r BFP[K592100+J61048] K^r Digestion of [R0010] C^r (EcoRI and SpeI) BFP[K592100+J61048] K^r (EcoRI and PstI) Electrophoresis of [R0010] C^r BFP[K592100+J61048] K^r Ligation of [R0010+B0034+pSB1K3] K^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K^r BFP[B0030+K592100+J61048+pSB1C3] C^r Transform of scFv-C[J23101+B0034+OmpA+scFv-C] A^r Ligation of OmpA[OmpA-linker+pSB1C3] C^r PCR of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] Cultivation of [pSB1C3] GFP[J23101+B0034+E0040+J61048+pSB1A3] A^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r BFP[J23101+B0034+OmpA+K592100+pSB1C3] C^r GBP[FadL-GBP+pSB6A1] A^r	27 PCR of GBP[FadL-GBP] Anti-Her 2[Herceptin+E1010] Gel extraction of GBP[FadL-GBP] Anti-Her 2[Herceptin+E1010] Transform of [R0010+B0034+pSB1K3] GBP[R0010+B0034+FadL-GBP+B0030+J61048+pSB1K3] BFP[B0030+K592100+J61048+pSB1C3] C^r PCR of GBP[J23110+B0034+FadL-GBP+E0040+J61048] C^r Gel extraction of GBP[J23110+B0034+FadL-GBP+E0040+J61048] C^r SDS-page of [pSB1C3] GFP[J23101+B0034+E0040+J61048+pSB1C3] C^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r BFP[J23101+B0034+OmpA+K592100+pSB1C3] C^r GBP[J23110+B0034+FadL-GBP+E0040+J61048+pSB1C3] C^r GBP[FadL-GBP+pSB6A1] A^r [pSB6A1]	28 Ligation of [R0010+B0034+pSB1K3] GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] K^r BFP[B0030+K592100+J61048+pSB1C3] C^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1C3] C^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A^r PCR of BFP[B0030+K592100+J61048+pSB1C3] C^r [R0010+B0034+pSB1K3] GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] K^r OmpA[J23110+B0034+OmpA+pSB3C5] C^r Digestion of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI) Electrophoresis of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI) SDS-page of [pSB1C3] BFP[J23101+B0034+OmpA+K592100+pSB1C3] C^r GFP[J23101+B0034+E0040+J61048+pSB1C3] C^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C^r [pSB6A1] GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A^r Transform of OmpA[OmpA-linker] C^r	29 Electrophoresis of OmpA[J23101+B0034+OmpA+pSB3C5] Transform of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A^r PCR of BFP[B0034+K592100+J61048+pSB1C3] C^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Electrophoresis of BFP[B0034+K592100+J61048+pSB1C3] C^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Digestion of Anti-Her 2[Herceptin-own] (EcoRI and PstI) Anti-Her 2[Herceptin-E1010] (EcoRI and PstI) GBP[GBP] (EcoRI and PstI) Ligation of [R0010+B0034+pSB1K3] K^r BFP[B0030+K592100+J61048] C^r GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] A^r GFP[R0010+B0030+E0040+J61048] A^r Electrophoresis of Anti-Her 2[Herceptin-own] (EcoRI and PstI) Anti-Her 2[Herceptin-E1010] (EcoRI and PstI) GBP[GBP] (EcoRI and PstI) [R0010+B0034+pSB1K3] K^r BFP[B0030+K592100+J61048] C^r GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] A^r GFP[R0010+B0030+E0040+J61048] A^r	30 Cultivation of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C^r Ligation of GBP[FadL-GBP+pSB1C3]C^r Digestion of Anti-Her 2[Herceptin own] (NcoI and PstI) [pSB1A3] (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) Cultivation of scFv-C[J23101+B0034+OmpA+scFv-C] A^r [J23101+B0034] K^r Digestion of scFv-B[scFv-B] K^r (NcoI and PstI) scFv-C[scFv-C] K^r (NcoI and PstI) OmpA[OmpA] C^r (EcoRI and PstI) Electrophoresis of Anti-Her 2[Herceptin own] (NcoI and PstI) [pSB1A3] (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) Plasmid purification of scFv-C[J23101+B0034+OmpA+scFv-C] A^r [J231-1+B0034] K^r Digestion of scFv-C[J23101+B0034+OmpA+scFv-C] A^r (EcoRI and SpeI) [J231-1+B0034] K^r (EcoRI and SpeI) Anti-Her 2[Herceptin-Own] (NcoI and PstI)	31 Electrophoresis of scFv-C[J23101+B0034+OmpA+scFv-C] A^r (EcoRI and SpeI) [J231-1+B0034] K^r (EcoRI and SpeI) Anti-Her 2[Herceptin-Own] (NcoI and PstI) Ligation of Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] A^r Transform of Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] A^r Electrophoresis of [pSB1C3] (XbaI and PstI) OmpA[OmpA] C^r (EcoRI and PstI) scFv-B[scFv-B] K^r (NcoI and PstI) scFv-C[scFv-C] K^r (NcoI and PstI)

Sun.	Mon.	Tue.	Wed.	Thu.	Fri.	Set.
						1 Digestion of Anti-Her 2[Herceptin-Web] (NcoI and PstI) Electrophoresis of Anti-Her 2[Herceptin-Web] (NcoI and PstI)
2 Electrophoresis of [pSB1C3] (XbaI and PstI) OmpA[OmpA] (EcoRI and PstI) scFv-B[scFv-B] K^r (NcoI and PstI) scFv-C[scFv-C] K^r (NcoI and PstI) OmpA[OmpA] (NcoI and PstI) Digestion of BFP[OmpA+K592100] C^r (EcoRI and NcoI)	3 Transform of [R0010+B0034+pSB1K3] K^r GBP[FadL-GBP+pSB1C3] C^r BFP[B0030+K592100+J61048] C^r Digestion of Anti-Her 2[Herceptin-Web] (NcoI and PstI) Ligation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r	4 Digestion of BFP[OmpA+K592100] C^r (EcoRI and NcoI) PCR of [R0010+B0034+pSB1K3] K^r BFP[B0030+K592100+J61048] C^r Transform of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r	5 Digestion of [pSB6A1] (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) PCR of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r Electrophoresis of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r Cultivation of BFP[B0030+K592100+J61048] C^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] PCR of GBP[FadL-GBP] C^r BFP[OmpA+K592100] C^r scFv-C[J23101+B0034+OmpA+scFv-C] A^r scFv-C[scFv-C+pSB1C3] C^r scFv-B[scFv-B+oSB1C3] C^r Digestion of scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C^r (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C^r (EcoRI and PstI) Transform of GBP[J23101+B0034+FadL-GBP]C^r scFv-B[scFv-B+pSB1C3] C^r scFv-C[scFv-C+pSB1C3] C^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C^r Gel extraction of [pSB6A1] (EcoRI and PstI) [OmpA] (NcoI and PstI) [pSB1C3] (EcoRI and PstI)	6 Cultivation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r Ligation of Anti-Her 2[OmpA+Herceptin-Web] C^r Anti-Her 2[OmpA+Herceptin-Own] C^r scFv-C[OmpA+scFv-C] C^r scFv-B[OmpA+scFv-B] C^r scFv-C[J23101+B0034+OmpA+scFv-C] C^r scFv-B[J23101+B0034+OmpA+scFv-B] C^r Transform of Anti-Her 2[OmpA+Herceptin-Web] C^r Anti-Her 2[OmpA+Herceptin-Own] C^r scFv-C[OmpA+scFv-C] C^r scFv-B[OmpA+scFv-B] C^r scFv-C[J23101+B0034+OmpA+scFv-C] C^r scFv-B[J23101+B0034+OmpA+scFv-B] C^r [pSB1C3] [pSB1A3] [pSB1K3] Plasmid purification of BFP[B0030+K592100+J61048] Digestion of BFP[B0030+K592100+J61048] (EcoRI and PstI) Cultivation of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C^r Ligation of [R0010+B0034] K^r GBP[GBP[J23101+B0034+FadL-GBP]+pSB1C3] C^r Digestion of [pSB1C3] (XbaI and PstI) Electrophoresis of GBP[J23101+B0034+FadL-GBP] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] C^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] C^r scFv-C[scFv-C] C^r scFv-B[scFv-B] C^r Plasmid purification of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r Digestion of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r (EcoRI and PstI) Ligation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A^r Electrophoresis of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A^r [B0034] [pSB1C3] Cultivation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r Transform of GBP[J23101+B0034+FadL-GBP] A^r Electrophoresis of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] C^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] C^r [pSB1C3] (XbaI and PstI) [pSB6A1] (XbaI and PstI) [pSB1C3] (XbaI and PstI) Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r Cultivation of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] C^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C^r GFP[J23101+B0034+E0040+J61048] A^r SDS-page of GFP[J23101+B0034+E0040+J61048] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] A^r [pSB6A1] GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] A^r Digestion of [pSB1C3] (XbaI and PstI) Anti-Her 2[Herceptin-Web] (NcoI and PstI) Anti-Her 2[Herceptin-Own] (NcoI and PstI) Ligation of [pSB1C3] (XbaI and PstI) Anti-Her 2[Herceptin-Web] C^r Anti-Her 2[Herceptin-Own] C^r BFP[J23101+B0034+OmpA+K592100] C^r	7 Plasmid purification of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C^r Digestion of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C^r PCR of scFv-B[J23101+B0034+OmpA+scFv-B] C^r scFv-C[J23101+B0034+OmpA+scFv-C] C^r Electrophoresis of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r (EcoRI and PstI) Cultivation of scFv-B[J23101+B0034+OmpA+scFv-B] C^r scFv-C[J23101+B0034+OmpA+scFv-C] C^r Transform of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r Plasmid purification of GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C^r Digestion of GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C^r (EcoRI and PstI) PCR of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A^r Electrophoresis of scFv-B[J23101+B0034+OmpA+scFv-B] C^r scFv-C[J23101+B0034+OmpA+scFv-C] C^r Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A^r OmpA[OmpA] (NcoI and PstI) [pSB1C3] (EcoRI and PstI) Ligation of scFv-C[OmpA+scFv-C] C^r scFv-B[OmpA+scFv-B] C^r Anti-Her 2[Herceptin-Own] C^r Anti-Her 2[Herceptin-Web] C^r Anti-Her 2[OmpA+Herceptin-Own] C^r Anti-Her 2[OmpA+Herceptin-Web] C Electrophoresis of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r (EcoRI and PstI) Anti-Her 2[Herceptin-Web] (NcoI and PstI) Anti-Her 2[Herceptin-Own] (NcoI and PstI) Transform of scFv-C[OmpA+scFv-C] C^r scFv-B[OmpA+scFv-B] C^r Anti-Her 2[Herceptin-Own] C^r Anti-Her 2[Herceptin-Web] C^r Anti-Her 2[OmpA+Herceptin-Own] C^r Anti-Her 2[OmpA+Herceptin-Web] C BFP[J23101+B0034+OmpA+K592100] C^r	8 Plasmid purification of scFv-C[J23101+OmpA+scFv-C] C^r scFv-B[J23101+OmpA+scFv-B] C^r Digestion of scFv-C[J23101+OmpA+scFv-C] C^r (EcoRI and PstI) scFv-B[J23101+OmpA+scFv-B] C^r (EcoRI and PstI) PCR of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A^r Cultivation of [pSB1K3] [pSB1C3] [pSB1A3] PCR of BFP[J23101+B0034+OmpA+K592100] C^r Transform of scFv-C[OmpA+scFv-C] C^r Electrophoresis of BFP[J23101+B0034+OmpA+K592100] C^r scFv-C[J23101+OmpA+scFv-C] C^r (EcoRI and PstI) scFv-B[J23101+OmpA+scFv-B] C^r (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) Digestion of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r (EcoRI and SpeI) OmpA[OmpA] C^r NcoI and PstI OmpA[OmpA+K592100] C^r NcoI and PstI Ligation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C^r Digestion of Anti-Her 2[Herceptin-Own] (EcoRI and PstI) Anti-Her 2[Herceptin-Web] (EcoRI and PstI) Electrophoresis of BFP[J23101+B0034+OmpA+K592100] C^r
9 Cultivation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A^r Plasmid purification of [pSB1C3] [pSB1K3] [pSB1A3] Digestion of [pSB1C3] (EcoRI and PstI) [pSB1K3] (EcoRI and PstI) [pSB1A3] (EcoRI and PstI) Transform of Anti-Her 2[Herceptin-Own] C^r Anti-Her 2[Herceptin-Web] C^r Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C^r [R0010+B0034] C^r BFP[J23101+B0034+OmpA+K592100] C^r Electrophoresis of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C^r (EcoRI and SpeI) OmpA[OmpA] C^r (NcoI and PstI) Digestion of [pSB1A3] (EcoRI and PstI) [pSB1K3] (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) RFP[B0030+E1010+J61048] C^r (XbaI and PstI) Electrophoresis of scFv-C[OmpA+scFv-C] K^r PCR of GBP[GBp+pSB1C3] C^r Digestion of BFP[B0030+K592100+J61048] C^r (XbaI and PstI)	10 Electrophoresis of GBP[GBp+pSB1C3] C^r [pSB1A3] (EcoRI and PstI) [pSB1K3] (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) Ligation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web+B0030+E1010+J61048] K^r Cultivation of [R0010+B0034] C^r BFP[B0030+K592100+J61048] C^r scFv-C[OmpA+scFv-C] K^r Digestion of [pSB1C3] (EcoRI and Pstl) Transform of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K^r Gel extraction of [pSB1C3] (EcoRI and PstI) PCR of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Anti-Her 2[Herceptin-Web] Anti-Her 2[Herceptin-Own]	11 Plasmid purification of [R0010+B0034] C^r scFv-C[OmpA+scFv-C] K^r BFP[OmpA+K592100] C^r Gel extraction of [pSB1C3] (EcoRI and PstI) Digestion of [R0010+B0034] C^r (EcoRI and SpeI) scFv-C[OmpA+scFv-C] K^r (EcoRI and PstI) BFP[OmpA+K592100] C^r (EcoRI and PstI) GBP[J23101+B0034+FadL-GBP] C^r (EcoRI and SpeI) Ligation of Anti-Her 2[Herceptin-Web] C^r PCR of scFv-B[OmpA+scFv-B] K^r Electrophoresis of [R0010+B0034] C^r (EcoRI and SpeI) scFv-C[OmpA+scFv-C] K^r (EcoRI and PstI) BFP[OmpA+K592100] C^r (EcoRI and PstI) GBP[J23101+B0034+FadL-GBP] C^r (EcoRI and SpeI) Ligation of GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A^r Electrophoresis of scFv-B[OmpA+scFv-B] K^r PCR of scFv-C[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K^r Transform of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K^r	12 Electrophoresis of scFv-B[OmpA+scFv-B] K^r Transform of Anti-Her 2[Herceptin-Web] C^r Anti-Her 2[Herceptin-Own] C^r Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A^r PCR of scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r Digestion of [pSB1C3] (EcoRI and PstI) Electrophoresis of scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r	13 Transform of GBP[J231010+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A^r Cultivation of [pSB1C3] scFv-B[OmpA-scFv-B] K^r Plasmid purification of [pSB1C3] scFv-B[OmpA-scFv-B] K^r Digestion of [pSB1C3] (EcoRI and PstI) scFv-B[OmpA-scFv-B] K^r (EcoRI and PstI) Electrophoresis of [pSB1C3] (EcoRI and PstI) Ligation of OmpA[R0010+B0034+OmpA+pSB1A3] A^r GBP[R0010+B0034+FadL-GBP+pSB1A3] A^r scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A^r scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A^r Electrophoresis of [pSB1C3] (EcoRI and PstI) scFv-B[OmpA-scFv-B] K^r (EcoRI and PstI)	14 Transform of OmpA[R0010+B0034+OmpA+pSB1A3] A^r GBP[R0010+B0034+FadL-GBP+pSB1A3] A^r scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A^r	15 PCR of OmpA[R0010+B0034+OmpA+pSB1A3] A^r GBP[R0010+B0034+FadL-GBP+pSB1A3] A^r scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A^r Ligation of scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C^r BFP[J23101+B0034+OmpA+K592100+pSB3C5] C^r PCR of GBP[R0010+B0034+FadL-GBP+pSB1A3] K^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K^r Transform of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K^r Electrophoresis of GBP[R0010+B0034+FadL-GBP+pSB1A3] K^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K^r Electrophoresis of GBP[FadL-GBP] (EcoRI and PstI)
16 PCR of GBP[R0010+B0034+FadL-GBP+pSB1A3] K^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] K^r Cultivation of OmpA[R0010+B0034+OmpA] Electrophoresis of GBP[R0010+B0034+FadL-GBP+pSB1A3] K^r GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] K^r	17 Ligation of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r Transform of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A^r	18 Plasmid purification of OmpA[R0010+B0034+OmpA+pSB1A3] A^r GBP[R0010+B0034+FadL-GBP+pSB1A3] A^r Digestion of BFP[OmpA+K592100] C^r (EcoRI and NcoI) Anti-Her 2[Herceptin-Own] (NcoI and SpeI) Anti-Her 2[Herceptin-Web] (NcoI and SpeI) Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A^r (EcoRI and SpeI) BFP[B0030+K592100+J61048] C^r (XbaI and PstI) OmpA[R0010+B0034+OmpA+pSB1A3] A^r (EcoRI and PstI) GBP[R0010+B0034+FadL-GBP+pSB1A3] A^r (EcoRI and PstI) Ligation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] C^r GBP[R0010+B0034+FadL-GBP+pSB1A3] A^r Transform of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] C^r GBP[R0010+B0034+FadL-GBP+pSB1A3] A^r GBP[J23101+B0034+fadL-GBP+B0030+E0040+J61048] C^r OmpA[R0010+B0034+OmpA+pSB1C3] C^r GBP[R0010+B0034+FadL-GBP+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ligation of scFv-B[R0010+B0034+OmpA+scFv-B] scFv-C[R0010+B0034+OmpA+scFv-C] Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own] Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web] GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] PCR of Anti-Her 2[Herceptin-Web] scFv-B[J23101+B0034+OmpA+scFv-B] scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C^r Anti-Her 2[Herceptin-Own] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r	19 Electrophoresis of Anti-Her 2[Herceptin-Web] scFv-B[J23101+B0034+OmpA+scFv-B] scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C^r Anti-Her 2[Herceptin-Own] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r scFv-C[OmpA+scFv-C] Anti-Her 2[Herceptin-Own] (NcoI and SpeI) Anti-Her 2[Herceptin-Web] (NcoI and SpeI) Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A^r (EcoRI and SpeI) BFP[B0030+K592100+J61048] C^r (XbaI and PstI) Transform of GBP[R0010+B034+FadL-GBP] A^r GBP[J23101+B0034+FadL-GBP] A^r GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C^r	20 PCR of GBP[FadL-GBP] K^r OmpA[OmpA] C^r Gel extraction of OmpA[OmpA] (EcoRI and SpeI) Plasmid purification of Anti-Her 2[Herceptin-Own] C^r scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r PCR of GBP[FadL-GBP] K^r OmpA[R0010+B0034+OmpA+pSB1C3] C^r GBP[R0010+B0034+GBP+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K^r GBP[R0010+B0034+GBP+B0030+E0040+J61048] scFv-B[R0010+B0034+OmpA+scFv-B] scFv-C[R0010+B0034+OmpA+scFv-C] Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own] Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web] Digestion of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A^r (EcoRI and PstI) Anti-Her 2[Herceptin-Own] C^r Electrophoresis of OmpA[R0010+B0034+OmpA+pSB1C3] C^r GBP[R0010+B0034+GBP+pSB1C3] C^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K^r GBP[R0010+B0034+GBP+B0030+E0040+J61048] Ligation of scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] K^r scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] K^r Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] K^r Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] K^r GBP[R0010+B0034+GBP+B0030+E0040+J61048pSB1A3] A^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K^r scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] K^r Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] K^r	21	22
23	24	25	26	27	28	29
30	31

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NCTU_Formosa APOllO

National Chaio Tung University
Engineering Building 6 EF455, 1001 University Road, Hsinchu 300, Taiwan, ROC.

@@ Line 9: / Line 9: @@
 a, abbr, acronym, address, big, cite, code,
 del, dfn, em, font, img, ins, kbd, q, s, samp,
-small, strike, strong, sub, tt, var,
+small, strike, strong, sub, sup, tt, var,
 b, u, i, center,
 dl, dt, dd, ol, ul, li,
@@ Line 540: / Line 540: @@
                              <B>15</B><br><br>
 							<b>Transformation of</b><br>GBP[FadL-GBP]<br>
-							<b>Cultivation of</b> <br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
+							Cultivation of <br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
                          </td>
                          <td>
                              <B>16</B><br><br>
-							<b>Cultivation of</b> <br>GBP[FadL-GBP]
+							Cultivation of <br>GBP[FadL-GBP]
 <br>
 <b>Plasmid purification of</b><br>OmpA[OmpA+pSB1K3] K<sup>r</sup>
@@ Line 773: / Line 773: @@
                          </td>
                          <td>
-                             <B>1</B><br><br>
+                             <B>1</B><br><br><b>PCR of</b> Transformation product:
+mini: Terminator[J61048]
+ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>
+GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+<b>Electrophoresis of</b>
+mini: Terminator[J61048]
+ligated product: OmpA[J23101+B0034+OmpA] A<sup>r</sup>
                          </td>
 						<td>
@@ Line 781: / Line 790: @@
                      <tr>
                          <td >
-                            <B>3</B><br><br>
+                            <B>3</B><br><br><b>Ligation of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
                          </td>
                          <td>
-                            <B>4</B><br><br>
+                            <B>4</B><br><br><b>Ligation of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+/4
+<b>Transformation off</b> ligated products:
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+<b>Digestion of </b>
+RFP[E1010+J61048] (K<sup>r</sup>) (XbaI and PstI)
+<b>PCR of</b> ligated products:
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+<b>Electrophoresis of</b> PCR products:
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
                          </td>
                          <td>
-                            <B>5</B><br><br>
+                            <B>5</B><br><br><b>Electrophoresis of</b>
+digested products:
+RFP[E1010+J61048] (K<sup>r</sup>) (XbaI and PstI)
+OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and PstI)
+GBP[FadL-GBP+J61048] K<sup>r</sup> (EcoRI and PstI)
+<b>PCR of</b>
+OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
+<b>Cultivation of</b> PCR product:
+RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
                          </td>
 						<td >
-                            <B>6</B><br><br>
+                            <B>6</B><br><br><b>Plasmid purification of</b>
+RFP[E1010+J61048+pSB1K3] K<sup>r</sup>
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+<b>Digestion of </b>
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+<b>Cultivation of</b> PCR product:
+OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
+<b>PCR of</b> backbone:
+[pSB1C3] C<sup>r</sup>
+[pSB1A3] A<sup>r</sup>
+[pSB1K3] K<sup>r</sup>
+<b>Electrophoresis of</b>
+PCR products: OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
+digested products: GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
                          </td>
                          <td>
-                             <B>7</B><br><br>
+                             <B>7</B><br><br><b>Digestion of </b>
+RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (XbaI and PstI)
+[pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+[pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+<b>Plasmid purification of</b>
+OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
+[pSB1K3] K<sup>r</sup>
+<b>Electrophoresis of</b> digested products:
+RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+????
+RFP[E1010+J61048+pSB1K3] K<sup>r</sup> (XbaI and PstI)
+[pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+[pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+[pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+<b>Ligation of</b>
+RFP[B0034+E1010+J61048+pSB1C3] C<sup>r</sup>
                          </td>
                          <td>
-                             <B>8</B><br><br>
+                             <B>8</B><br><br><b>Electrophoresis of</b> digested products:
+OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and PstI)
+<b>Transformation off</b> ligated products:
+RFP[B0034+E1010+J61048+pSB1C3] C<sup>r</sup>
+<b>PCR of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+<b>Electrophoresis of</b> PCR products:
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+<b>Transformation off</b>
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+[B0030] A<sup>r</sup>
+[J23110] A<sup>r</sup>
                          </td>
 						<td >
-                             <B>9</B><br><br>
+                             <B>9</B><br><br><b>PCR of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+[B0030] A<sup>r</sup>
+[J23110] A<sup>r</sup>
+<b>Electrophoresis of</b> PCR products:
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+[B0030] A<sup>r</sup>
+[J23110] A<sup>r</sup>
+<b>Cultivation of</b>
+[B0030] A<sup>r</sup>
+[J23110] A<sup>r</sup>
+<b>Ligation of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
                          </td>
                      </tr>
                      <tr>
                          <td >
-                             <B>10</B><br><br>
+                             <B>10</B><br><br><b>Electrophoresis of</b>
+[B0030] A<sup>r</sup>
+<b>Cultivation of</b>
+[B0030] A<sup>r</sup>
+<b>Plasmid purification of</b>
+[J23110] A<sup>r</sup>
+[B0030] A<sup>r</sup>
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+<b>Digestion of </b>
+[B0030] A<sup>r</sup> (EcoRI and SpeI)
+[J23110] A<sup>r</sup> (EcoRI and SpeI)
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
+<b>Electrophoresis of</b> digested products:
+[B0030] A<sup>r</sup> (EcoRI and SpeI)
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
                          </td>
                          <td>
-                             <B>11</B><br><br>
+                             <B>11</B><br><br><b>Electrophoresis of</b> digested products:
+[J23110] A<sup>r</sup> (EcoRI and SpeI)
+<b>Ligation of</b>
+RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
+GFP[B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
+<b>Transformation off</b>
+RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
+GFP[B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
+<b>PCR of</b>
+RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
+GFP[B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
                          </td>
                          <td>
-                             <B>12</B><br><br>
+                             <B>12</B><br><br><b>PCR of</b> ligated products:
+RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
+<b>Cultivation of</b> PCR products:
+RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
+<b>Plasmid purification of</b>
+RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
+<b>Digestion of </b>
+RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and SpeI)
+RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and PstI)
+<b>Electrophoresis of</b>
+digested products:
+RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and SpeI)
+RFP[B0030+E1010+J61048] C<sup>r</sup> (EcoRI and PstI)
+PCR products: RFP[B0030+E1010+J61048+pSB1C3] C<sup>r</sup>
                          </td>
 						<td >
-                             <B>13</B><br><br>
+                             <B>13</B><br><br><b>Ligation of</b>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
                          </td>
                          <td>
-                             <B>14</B><br><br>
+                             <B>14</B><br><br><b>Cultivation of</b>
+OmpA[J23101+B0034+OmpA+pSB1A3] A<sup>r</sup>
+<b>Transformation off</b>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+<b>Electrophoresis of</b> digested products:
+GBP[J23101+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
+[pSB1K3] K<sup>r</sup> (EcoRI and PstI)
                          </td>
                          <td>
-                             <B>15</B><br><br>
+                             <B>15</B><br><br><b>Digestion of </b>
+GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+<b>Ligation of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
                          </td>
 						<td >
-                            <B>16</B><br><br>
+                            <B>16</B><br><br><b>Transformation off</b>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+<b>Electrophoresis of</b>
+GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+GBP[B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (XbaI and PstI)
+<b>Ligation of</b>
+GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
+<b>Transformation off</b>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
                          </td>
                      </tr>
                      <tr>
                          <td >
-                             <B>17</B><br><br>
+                             <B>17</B><br><br><b>Ligation of</b>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+<b>PCR of</b>
+GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+<b>Electrophoresis of</b> PCR products:
+GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
                          </td>
                          <td>
-                            <B>18</B><br><br>
+                            <B>18</B><br><br><b>Cultivation of</b>
+GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
+<b>Plasmid purification of</b>
+GBP[B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
+<b>Digestion of </b>
+GBP[B0034+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
+RFP[B0030+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
+GFP[B0030+E0040+J61048]
+<b>Transformation off</b>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+GFP[B0030+E0040+J61048]
                          </td>
                          <td>
-                             <B>19</B><br><br>
+                             <B>19</B><br><br><b>Ligation of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+<b>PCR of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+<b>Electrophoresis of</b>
+digested products:
+RFP[B0030+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
+GBP[B0034+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
+PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+<b>Transformation off</b>
+scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
+scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
+GFP[B0030+E0040+J61048]
+<b>Cultivation of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
                          </td>
 						<td >
-                             <B>20</B><br><br>
+                             <B>20</B><br><br><b>Cultivation of</b>
+OmpA[J23101+B0034+OmpA] A<sup>r</sup>
+scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
+scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
+<b>Plasmid purification of</b>
+OmpA[J23101+B0034+OmpA] A<sup>r</sup>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
+scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
+<b>Digestion of </b>
+OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and Pst1)
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+OmpA[J23101+B0034+OmpA] A<sup>r</sup> (NcoI and PstI)
+scFv-B[scFv-B] A<sup>r</sup> (EcoRI and PstI)
+scFv-C[scFv-C] A<sup>r</sup> (EcoRI and PstI)
+<b>Ligation of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+<b>Transformation off</b>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+[pSB3C5] 抗性
+<b>Electrophoresis of</b>
+OmpA[J23101+B0034+OmpA] A<sup>r</sup> (EcoRI and PstI)
                          </td>
                          <td>
-                             <B>21</B><br><br>
+                             <B>21</B><br><br><b>Digestion of </b>
+scFv-B[scFv-B] A<sup>r</sup> (EcoRI and PstI)
+scFv-C[scFv-C] A<sup>r</sup> (EcoRI and PstI)
+<b>Electrophoresis of</b>
+OmpA[J23101+B0034+OmpA] A<sup>r</sup> (NcoI and PstI)
+scFv-B[scFv-B] A<sup>r</sup> (EcoRI and PstI)
+scFv-C[scFv-C] A<sup>r</sup> (EcoRI and PstI)
+<b>Ligation of</b>
+scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
+scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
+scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
+scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
                          </td>
                          <td>
-                             <B>22</B><br><br>
+                             <B>22</B><br><br><b>Cultivation of</b>
+[pSB3C5] C<sup>r</sup>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+<b>Transformation off</b>
+GBP[J23110+B0034+GBP] A<sup>r</sup>
+<b>PCR of</b>
+scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
+scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
+scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
+scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
+<b>Electrophoresis of</b>
+scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
+scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
+scFv-B[scFv-B+pSB1K3] K<sup>r</sup>
+scFv-C[scFv-C+pSB1K3] K<sup>r</sup>
+<b>Ligation of</b>
+scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
+<b>Transform of</b>
+scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
                          </td>
 						<td >
-                             <B>23</B><br><br>
+                             <B>23</B><br><br><b>Plasmid purification of</b>
+[pSB3C5] C<sup>r</sup>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+<b>Digestion of </b>
+[pSB3C5] C<sup>r</sup> (EcoRI and PstI)
+[J23110+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+<b>PCR of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+<b>Transformation off</b>
+scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
                          </td>
                      </tr>
 					<tr>
                          <td >
-                             <B>24</B><br><br>
+                             <B>24</B><br><br><b>Electrophoresis of</b> digested products:
+[pSB3C5] C<sup>r</sup> (EcoRI and PstI)
+[J23110+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+<b>Cultivation of</b>
+PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
+scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
+<b>Plasmid purification of</b>
+scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
+GFP[B0030+E0040+J61048]
+<b>Digestion of </b>
+[J23101+B0034] K<sup>r</sup> (EcoRI and SpeI)
+<b>Ligation of</b>
+OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
+<b>PCR of</b>
+GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts
+<b>Electrophoresis of</b>
+GBP[J23110+B0034+GBP] A<sup>r</sup>
                          </td>
                          <td>
-                            <B>25</B><br><br>
+                            <B>25</B><br><br><b>Transformation off</b>
+OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
+<b>Cultivation of</b>
+GBP[J23110+B0034+FadL-GBP] A<sup>r</sup>
+<b>Digestion of </b>
+GBP[B003+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
+scFv-B[scFv-B+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+scFv-C[scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+GFP[B0030+E0040+J61048]  (XbaI and PstI)
+<b>Electrophoresis of</b>
+GFP[B0030+E0040+J61048]
+scFv-B[scFv-B+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+scFv-C[scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+GFP[B0030+E0040+J61048]  (XbaI and PstI)
+<b>Cultivation of</b>
+scFv-B[scFv-B+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+scFv-C[scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+GFP[B0030+E0040+J61048]  (XbaI and PstI)
                          </td>
                          <td>
-                             <B>26</B><br><br>
+                             <B>26</B><br><br><b>Ligation of</b>
+OmpA[J23110+B0034+OmpA+pSB1A3] A<sup>r</sup>
+GBP[J23110+B0034+GBP+pSB1A3] A<sup>r</sup>
+<b>Gel extraction of</b>
+[J23110] A<sup>r</sup> (SpeI and PstI)
+<b>Plasmid purification of</b>
+GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts
+<b>Digestion of </b>
+GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts (EcoRI and SpeI)
                          </td>
 						<td >
-                             <B>27</B><br><br>
+                             <B>27</B><br><br><b>PCR of</b>
+[J23101+B0034+OmpA+pSB3C5]
+<b>Electrophoresis of</b>
+GBP[J23110+B0034+GBP] A<sup>r</sup> by two pA<sup>r</sup>ts (EcoRI and SpeI)
+GBP[B0034+FadL-GBP] C<sup>r</sup> (XbaI and PstI)
+[J23110] A<sup>r</sup> (SpeI and PstI)
                          </td>
                          <td>
-                             <B>28</B><br><br>
+                             <B>28</B><br><br><b>Ligation of</b>
+scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] 抗性??
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
+<b>Transform of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
+scFv-B[scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[scFv-C+pSB1A3] A<sup>r</sup>
+<b>PCR of</b>
+[J23101+B003+OmpA+pSB3C5] + primer(EX-OmpA and OmpA-S)
+OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
+Plasmid purification in gel slices of PCR products:
+OmpA[EX-OmpA-S]
+<b>Gel extraction of</b>
+OmpA[OmpA] (EcoRI and SpeI)
+<b>Transformation off</b>
+OmpA[J23101+B0034+OmpA+pSB3C5]
                          </td>
                          <td>
-                             <B>29</B><br><br>
+                             <B>29</B><br><br><b>Plasmid purification of</b>
+[J23110] A<sup>r</sup>
+[pSB1C3]
+<b>Digestion of </b>
+[J23110] A<sup>r</sup> (EcoRI and SpeI)
+[pSB1C3] (EcoRI and SpeI)
+<b>Ligation of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+<b>Electrophoresis of</b>
+[J23110] A<sup>r</sup> (EcoRI and SpeI)
+[pSB1C3] (EcoRI and SpeI)
+<b>Transform of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
                          </td>
 						<td >
-                             <B>30</B><br><br>
+                             <B>30</B><br><br><b>PCR of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
                          </td>
                      </tr>
 					<tr>
                          <td >
-                             <B>31</B><br><br>
+                             <B>31</B><br><br><b>Cultivation of</b>
+[J23110+B0034] K<sup>r</sup>
+GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
+<b>Plasmid purification of</b>
+[J23110+B0034] K<sup>r</sup>
+GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup>
+<b>Digestion of </b>
+[J23110+B0034] K<sup>r</sup> (EcoRI and PstI)
+GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+[J23110+B0034] (EcoRI and SpeI) K<sup>r</sup>
+[J23101+B0034] (EcoRI and SpeI) K<sup>r</sup>
+OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
+[pSB1K3] (EcoRI and PstI)
+<b>Electrophoresis of</b>
+[J23110+B0034] K<sup>r</sup> (EcoRI and PstI)
+GFP[J23110+B0034+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+<b>Ligation of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
                          </td>
                          <td>
@@ Line 927: / Line 1,393: @@
 						</td>
                          <td >
-                             <B>1</B><br><br>
+                             <B>1</B><br><br><b>Electrophoresis of</b>
+[J23110+B0034] (EcoRI and SpeI) K<sup>r</sup>
+[J23101+B0034] (EcoRI and SpeI) K<sup>r</sup>
+OmpA[OmpA] (NcoI and PstI)
+GFP[K1489002+E0040] K<sup>r</sup>
+<b>Digestion of </b>
+[pSB1C3] (EcoRI and PstI)
+[pSB1A3] (EcoRI and PstI)
+OmpA[OmpA] (EcoRI and NcoI)
+[pSB3C5] (EcoRI and PstI)
+<b>Ligation of</b>
+GFP[J23101+B0034+K1489002+E0040+pSB3C5] (C<sup>r</sup>)
+GFP[J23110+B0034+K1489002+E0040+pSB3C5] (C<sup>r</sup>)
+<b>Electrophoresis of</b> digested product:
+[pSB1C3] (EcoRI and PstI)
+[pSB1A3] (EcoRI and PstI)
+OmpA[OmpA] (EcoRI and NcoI)
+<b>Cultivation of</b>
+BFP[OmpA+K592100]
+<b>PCR of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
+<b>Electrophoresis of</b>
+GBP[J23101+B0034+FadL-GBP+pSB1K3] K<sup>r</sup>
                          </td>
                          <td>
-                             <B>2</B><br><br>
+                             <B>2</B><br><br><b>PCR of</b>
+OmpA[EX-OmpA-S+pSB1C3] C<sup>r</sup>
+<b>Electrophoresis of</b>
+OmpA[EX-OmpA-S+pSB1C3] C<sup>r</sup>
+OmpA[OmpA] (EcoRI and NcoI)
+<b>Ligation of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
+<b>Digestion of </b>
+[pSB1A3] (EcoRI and NcoI)
+<b>Electrophoresis of</b> digested products:
+[pSB1A3] (EcoRI and NcoI)
                          </td>
                          <td>
-                            <B>3</B><br><br>
+                            <B>3</B><br><br><b>Ligation of</b>
+OmpA[OmpA+psB1C3] C<sup>r</sup>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+<b>Transform of</b>
+OmpA[OmpA+psB1C3] C<sup>r</sup>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+<b>Digestion of </b>
+BFP[OmpA+ K592100] (NcoI and PstI)
+OmpA[OmpA] (NcoI and PstI)
                          </td>
 						<td >
-                            <B>4</B><br><br>
+                            <B>4</B><br><br><b>Cultivation of</b>
+[J23110+pSB1A3] A<sup>r</sup>
+<b>Transform of</b>
+[pSB1C3]
+<b>Digestion of </b>
+[pSB1C3] (EcoRI and PstI)
+<b>Ligation of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
+OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
+<b>Transform of</b>
+[B0034+pSB1A3] A<sup>r</sup>
                          </td>
                          <td>
-                            <B>5</B><br><br>
+                            <B>5</B><br><br><b>Transform of</b>
+OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
+<b>Plasmid purification of</b>
+[J23110+pSb1A3] A<sup>r</sup>
+<b>Digestion of </b>
+[J23110+pSb1A3] A<sup>r</sup>
+<b>PCR of</b>
+[J23110+pSb1A3] A<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+<b>Transform of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
+<b>Electrophoresis of</b>
+[pSB1C3]
+[B0034+pSB1A3] A<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
                          </td>
                          <td>
-                             <B>6</B><br><br>
+                             <B>6</B><br><br><b>Cultivation of</b>
+[pSB1C3]
+<b>Digestion of </b>
+[pSB1C3] (EcoRI and SpeI)
                          </td>
@@ Line 948: / Line 1,510: @@
                      <tr>
 						<td>
-                             <B>7</B><br><br>
+                             <B>7</B><br><br><b>Gel extraction of</b>
+OmpA[EX-OmpA-S] 抗性
+[pSB1C3] (EcoRI and SpeI)
+<b>Gel extraction of</b>
+[pSB1C3] (EcoRI and SpeI)
+<b>Transform of</b>
+GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
+[pSB1A3]
+OmpA[J23101+B0034+OmpA] A<sup>r</sup>
+<b>Ligation of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
+<b>Cultivation of</b>
+[pSB1C3]
                          </td>
                          <td >
-                            <B>8</B><br><br>
+                            <B>8</B><br><br><b>Transform of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
+[pSB3K3]
+[pSB6A1]
+<b>Plasmid purification of</b>
+[pSB1C3]
+<b>Digestion of </b>
+OmpA[EX-OmpA-S] (EcoRI and SpeI)
+<b>Electrophoresis of</b>
+[pSB1C3] (EcoRI and SpeI)
+<b>Transform of</b>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
                          </td>
                          <td>
-                            <B>8</B><br><br>
+                            <B>9</B><br><br><b>Ligation of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
+<b>PCR of</b>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
+<b>Cultivation of</b>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+<b>Plasmid purification of</b>
+[pSB3K3]
+[pSB6A1]
+<b>Digestion of </b>
+[pSB3K3] (EcoRI and PstI)
+[pSB6A1] (EcoRI and PstI)
                          </td>
                          <td>
-                            <B>10</B><br><br>
+                            <B>10</B><br><br><b>Transform of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
+<b>Plasmid purification of</b>
+[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+<b>Digestion of </b>
+[J23101+B0034+OmpA+K592100] C<sup>r</sup> (EcoRI and PstI)
+<b>Electrophoresis of</b>
+[pSB3K3] (EcoRI and PstI)
+[pSB6A1] (EcoRI and PstI)
+[pSB1A3] (EcoRI and PstI)
+scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and PstI)
+scFv-C[J23101+B0034+OmpA+scFv-C] (EcoRI and PstI)
+<b>Transform of</b>
+[J23110+B0034] K<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3]
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3]
+<b>Ligation of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
+<b>Electrophoresis of</b>
+[pSB3K3]
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
                          </td>
 						<td >
-                            <B>11</B><br><br>
+                            <B>11</B><br><br><b>Transform of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
+[J23110+B0034+pSB1C3] C<sup>r</sup>
+<b>Ligation of</b>
+[J23110+B0034+pSB1K3] K<sup>r</sup>
+[J23110+B0034+pSB1C3] C<sup>r</sup>
+<b>PCR of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
+<b>Electrophoresis of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
+<b>Cultivation of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
                          </td>
                          <td>
-                             <B>12</B><br><br>
+                             <B>12</B><br><br><b>PCR of</b>
+[J23110+B0034+pSB1C3] C<sup>r</sup>
+[B0034+pSB1A3] A<sup>r</sup>
+<b>Plasmid purification of</b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup>
+<b>Ligation of</b>
+[J23110+B0034+pSB1C3] C<sup>r</sup>
+<b>Transform of</b>
+[J23110+B0034+pSB1C3] C<sup>r</sup>
                          </td>
                          <td>
-                             <B>13</B><br><br>
+                             <B>13</B><br><br><b>Electrophoresis of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
                          </td>
 					 </tr>
@@ Line 972: / Line 1,648: @@
 					<tr><td >
-                             <B>14</B><br><br>
+                             <B>14</B><br><br><b>SDS-page of</b>
+[pSB1C3]
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+[pSB3C5]
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
+scFv-B[OmpA+scFv-B]
+scFv-C[OmpA+scFv-C]
+<b>Electrophoresis of</b>
+[J23110]
+[B0034+FadL-GBP+B0030+E1010+J61048] 抗性 (XbaI and PstI)
+<b>Ligation of</b>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3K3] C<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] C<sup>r</sup>
+<b>Digestion of </b>
+OmpA[OmpA+pSB1C3] C<sup>r</sup> (EcoRI and SpeI)
+OmpA[OmpA+pSB1C3] C<sup>r</sup> (EcoRI and PstI)
+<b>Transform of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Ligation of</b>
+GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Transform of</b>
+GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td>
                          <td >
@@ Line 978: / Line 1,685: @@
                          </td>
                          <td>
-                             <B>16</B><br><br>
+                             <B>16</B><br><br><b>Transform of</b>
+[pSB3C5]
                          </td>
                          <td>
-                             <B>17</B><br><br>
+                             <B>17</B><br><br><b>Cultivation of</b>
+[pSB3C5]
                          </td>
 						<td >
-                             <B>18</B><br><br>
+                             <B>18</B><br><br><b>Plasmid purification of</b>
+[pSB3C5]
+<b>Digestion of </b>
+[pSB3C5]( EcoRI and PstI)
+<b>Electrophoresis of</b>
+[pSB3C5]( EcoRI and PstI)
+<b>Ligation of</b>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
                          </td>
                          <td>
-                             <B>19</B><br><br>
+                             <B>19</B><br><br><b>Transform of</b>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
                          </td>
                          <td>
-                             <B>20</B><br><br>
+                             <B>20</B><br><br><b>PCR of</b>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
+<b>Electrophoresis of</b>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
                          </td>
@@ Line 1,001: / Line 1,734: @@
                          </td>
                          <td>
-                            <B>23</B><br><br>
+                            <B>23</B><br><br><b>PCR of</b>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
+<b>Cultivation of</b>
+[pSB3C5]
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+BFP[OmpA+K592100] 抗性
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1]
+[psB6A1]
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+[pSB1A3]
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+<b>Ligation of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td>
                          <td>
-                             <B>24</B><br><br>
+                             <B>24</B><br><br><b>Plasmid purification of</b>
+[pSB3C5]
                          </td>
 						<td >
-                             <B>25</B><br><br>
+                             <B>25</B><br><br><b>Cultivation of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+<b>Transform of</b>
+[J23101+B0034+pSB1K3] K<sup>r</sup>
                          </td>
                          <td>
@@ Line 1,013: / Line 1,768: @@
                          </td>
                          <td>
-                             <B>27</B><br><br>
+                             <B>27</B><br><br><b>Digestion of </b>
+OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
+<b>Electrophoresis of</b>
+GBP[FadL-GBP] 抗性 (EcoRI and PstI)
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
+OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
                          </td>
                      </tr>
 					<tr><td >
-                             <B>28</B><br><br>
+                             <B>28</B><br><br><b>Cultivation of</b>
+BFP[J23101+B0034+OmpA+K592100+pSB1A3] A<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] C<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+[pSB6A1]
+[pSB3C5]
+[pSB1C3]
+[pSB1A3]
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+<b>Electrophoresis of</b>
+[J23101+B0034] K<sup>r</sup>
+GBP[FadL-GBP] 抗性 (EcoRI and PstI)
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A<sup>r</sup> (EcoRI and PstI)
+OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
+BFP[J23101+B0034+OmpA+K592100] A<sup>r</sup> (EcoRI and PstI)
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
+[pSB6A1] (EcoRI and PstI)
+OmpA[OmpA] C<sup>r</sup> (XbaI and PstI)
+[E0040] A<sup>r</sup> (XbaI and PstI)
+<b>Ligation of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+[B0034+E0040+pSB1K3] K<sup>r</sup>
+GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
+GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
+GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
+<b>Digestion of </b>
+BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup> (XbaI and PstI)
+<b>SDS-page of</b>
+[pSB1A3]
+[pSB1C3]
+BFP[J23101+B0034+OmpA+K592100+pSB1A3] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+[pSB3C5]
+[pSB6A1]
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] C<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Transform of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
+GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
                          </td>
                          <td >
-                             <B>29</B><br><br>
+                             <B>29</B><br><br><b>PCR of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
+GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+<b>Electrophoresis of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td>
                          <td>
-                            <B>30</B><br><br>
+                            <B>30</B><br><br><b>Electrophoresis of</b>
+scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td>
                          <td>
@@ Line 1,077: / Line 1,904: @@
 					</td>
                          <td >
-                             <B>1</B><br><br>
+                             <B>1</B><br><br><b>Cultivation of</b>
-							PCR<br>
+[pSB6A1]
-							transform
+scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Transform of</b>
+scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+<b>Digestion of </b>
+GBP[]FadL-GBP] 抗性 (EcoRI and PstI)
                          </td>
                          <td>
-                             <B>2</B><br><br>
+                             <B>2</B><br><br><b>Digestion of </b>
+[J23110] A<sup>r</sup> (SpeI and PstI)
+Plasmid purification in gel slices of
+scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Digestion of </b>
+scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
+<b>Electrophoresis of</b>
+scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
                          </td>
                          <td>
-                            <B>3</B><br><br>
+                            <B>3</B><br><br><b>Gel extraction of</b>
+[J23110] A<sup>r</sup> (SpeI and PstI)
+[pSB6A1] (EcoRI and PstI)
+<b>Transform of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+<b>Electrophoresis of</b>
+GBP[FadL-GBP] (EcoRI and Pstl)
+<b>Ligation of</b>
+GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
+GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
+GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
                          </td>
 						<td >
-                            <B>4</B><br><br>
+                            <B>4</B><br><br><b>Transform of</b>
+GBP[FadL-GBP+pSB3C5] C<sup>r</sup>
+GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
+GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+<b>Electrophoresis of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+<b>Cultivation of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+[J23101+B0034+pSB1K3] K<sup>r</sup>
                          </td>
@@ Line 1,094: / Line 1,974: @@
                      </tr>
                      <tr><td>
-                            <B>5</B><br><br>
+                            <B>5</B><br><br><b>Plasmid purification of</b>
+[J23101+B0034+pSB1K3] K<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
                          </td><td>
-                             <B>6</B><br><br>
+                             <B>6</B><br><br><b>Cultivation of</b>
+[pSB1C3]
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+[pSB6A1]
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+[pSB1A3]
+GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
                          </td>
 						<td>
-                             <B>7</B><br><br>
+                             <B>7</B><br><br><b>Digestion of </b>
+[J23101+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+<b>SDS-page of</b>
+[pSB1C3]
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+[pSB6A1]
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+[pSB1A3]
+GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup>
+<b>Ligation of</b>
+[J23110+B0034] A<sup>r</sup>
+<b>Electrophoresis of</b>
+[J23101+B0034+pSB1K3] K<sup>r</sup> (EcoRI and PstI)
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td>
                          <td >
-                            <B>8</B><br><br>
+                            <B>8</B><br><br><b>Plasmid purification of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Transform of</b>
+[J23110+B0034] A<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100]
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100]
+<b>Electrophoresis of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
                          </td>
                          <td>
-                            <B>9</B><br><br>
+                            <B>9</B><br><br><b>Electrophoresis of</b>
+scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+scFv-B+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
+[pSB3C5]
+BFP[J23101+B0034+OmpA+scFv-B+B0030+e0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Cultivation of</b>
+scFv-B[J23110+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td>
                          <td>
-                            <B>10</B><br><br>
+                            <B>10</B><br><br><b>Plasmid purification of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td>
 						<td >
-                            <B>11</B><br><br>
+                            <B>11</B><br><br><b>PCR of</b>
+[J23110+B0034] A<sup>r</sup>
+<b>Electrophoresis of</b>
+[J23110+B0034] A<sup>r</sup>
                          </td>
                      </tr>
                      <tr><td>
-                             <B>12</B><br><br>
+                             <B>12</B><br><br><b>Cultivation of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+[J23110+B0034] A<sup>r</sup>
+<b>Plasmid purification of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Digestion of </b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
                          </td>
                          <td>
-                             <B>13</B><br><br>
+                             <B>13</B><br><br><b>Plasmid purification of</b>
+[J23110+B0034] A<sup>r</sup>
+<b>Transform of</b>
+[R0010]
+<b>Electrophoresis of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
                          </td>
 						<td >
-                             <B>14</B><br><br>
+                             <B>14</B><br><br><b>Digestion of </b>
+[J23110+B0034] A<sup>r</sup> (EcoRI and PstI)
+GBP[FadL-GBP] K<sup>r</sup> (XbaI and PstI)
+OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
+<b>Electrophoresis of</b>
+[J23110+B0034] A<sup>r</sup> (EcoRI and PstI)
+GBP[FadL-GBP] K<sup>r</sup> (XbaI and PstI)
+OmpA[OmpA] C<sup>r</sup> (EcoRI and SpeI)
+<b>Transform of</b>
+[R0010] C<sup>r</sup>
+<b>Cultivation of</b>
+[pSB1C3]
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
                          </td>
                          <td >
-                             <B>15</B><br><br>
+                             <B>15</B><br><br><b>Digestion of </b>
+[pSB6A1]
+[pSB3C5]
+Anti-Her 2[Herceptin+E1010]
+[J23101+B0034+OmpA+scFv-C]
+[J23101+B0034+OmpA+scFv-B]
+<b>Cultivation of</b>
+[pSB6A1]
+[R0010]
+<b>PCR of</b>
+OmpA[OmpA-linker] (EcoRI and PstI)
+<b>Transform of</b>
+[J23110+B0034] K<sup>r</sup>
+BFP[K592100+J61048] K<sup>r</sup>
                          </td>
                          <td>
-                             <B>16</B><br><br>
+                             <B>16</B><br><br><b>Gel extraction of</b>
+OmpA[OmpA-linker] (EcoRI and PstI)
+<b>Ligation of</b>
+OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+<b>Digestion of </b>
+OmpA[OmpA-linker] (EcoRI and PstI)
+<b>Transform of</b>
+OmpA[J23101+B0034+OmpA+pSB3C5] C<sup>r</sup>
                          </td>
                          <td>
-                             <B>17</B><br><br>
+                             <B>17</B><br><br><b>Electrophoresis of</b>
+OmpA[OmpA-linker] (EcoRI and PstI)
+Anti-Her 2[Herceptin+E1010] (EcoRI and PstI)
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
                          </td>
 						<td >
-                             <B>18</B><br><br>
+                             <B>18</B><br><br><b>Ligation of</b>
+OmpA[OmpA-linker+pSB1C3] C<sup>r</sup>
+Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
                          </td>
@@ Line 1,140: / Line 2,150: @@
                      </tr>
                      <tr><td>
-                             <B>19</B><br><br>
+                             <B>19</B><br><br><b>Transform of</b>
+OmpA[OmpA-linker+pSB1C3] C<sup>r</sup>
+Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
                          </td><td>
                              <B>20</B><br><br>
@@ Line 1,154: / Line 2,167: @@
                          </td>
                          <td>
-                             <B>24</B><br><br>
+                             <B>24</B><br><br><b>Transform of</b>
+OmpA[OmpA-linker+pSB1C3] C<sup>r</sup>
+Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
+<b>PCR of</b>
+OmpA[OmpA-linker+pSB1C3] C<sup>r</sup>
+Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
+<b>Electrophoresis of</b>
+OmpA[OmpA-linker+pSB1C3] C<sup>r</sup>
+Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
                          </td>
 						<td >
-                             <B>25</B><br><br>
+                             <B>25</B><br><br><b>Cultivation of</b>
+[R0010] C<sup>r</sup>
+BFP[K592100+J61048] K<sup>r</sup>
+<b>PCR of</b>
+OmpA[OmpA-linker+pSB1C3] C<sup>r</sup>
+Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
+<b>Electrophoresis of</b>
+OmpA[OmpA-linker+pSB1C3] C<sup>r</sup>
+Anti-Her 2[Herceptin+E1010+pSB1C3] C<sup>r</sup>
                          </td>
@@ Line 1,163: / Line 2,198: @@
                      </tr>
 					<tr><td>
-                             <B>26</B><br><br>
+                             <B>26</B><br><br><b>Plasmid purification of</b>
+[R0010] C<sup>r</sup>
+BFP[K592100+J61048] K<sup>r</sup>
+<b>Digestion of </b>
+[R0010] C<sup>r</sup> (EcoRI and SpeI)
+BFP[K592100+J61048] K<sup>r</sup> (EcoRI and PstI)
+<b>Electrophoresis of</b>
+[R0010] C<sup>r</sup>
+BFP[K592100+J61048] K<sup>r</sup>
+<b>Ligation of</b>
+[R0010+B0034+pSB1K3] K<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
+BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
+<b>Transform of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
+<b>Ligation of</b>
+OmpA[OmpA-linker+pSB1C3] C<sup>r</sup>
+<b>PCR of</b>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048]
+<b>Cultivation of</b>
+[pSB1C3]
+GFP[J23101+B0034+E0040+J61048+pSB1A3] A<sup>r</sup> scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
+GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
                          </td><td>
-                             <B>27</B><br><br>
+                             <B>27</B><br><br><b>PCR of</b>
+GBP[FadL-GBP]
+Anti-Her 2[Herceptin+E1010]
+<b>Gel extraction of</b>
+GBP[FadL-GBP]
+Anti-Her 2[Herceptin+E1010]
+<b>Transform of</b>
+[R0010+B0034+pSB1K3]
+GBP[R0010+B0034+FadL-GBP+B0030+J61048+pSB1K3]
+BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
+<b>PCR of</b>
+GBP[J23110+B0034+FadL-GBP+E0040+J61048] C<sup>r</sup>
+<b>Gel extraction of</b>
+GBP[J23110+B0034+FadL-GBP+E0040+J61048] C<sup>r</sup>
+<b>SDS-page of</b>
+[pSB1C3]
+GFP[J23101+B0034+E0040+J61048+pSB1C3] C<sup>r</sup> scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+E0040+J61048+pSB1C3] C<sup>r</sup>
+GBP[FadL-GBP+pSB6A1] A<sup>r</sup>
+[pSB6A1]
                          </td>
 						<td >
-                             <B>28</B><br><br>
+                             <B>28</B><br><br><b>Ligation of</b>
+[R0010+B0034+pSB1K3]
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] K<sup>r</sup>
+BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1C3] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A<sup>r</sup>
+<b>PCR of</b>
+BFP[B0030+K592100+J61048+pSB1C3] C<sup>r</sup>
+[R0010+B0034+pSB1K3]
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] K<sup>r</sup>
+OmpA[J23110+B0034+OmpA+pSB3C5] C<sup>r</sup>
+<b>Digestion of </b>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI)
+<b>Electrophoresis of</b>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI)
+<b>SDS-page of</b>
+[pSB1C3]
+BFP[J23101+B0034+OmpA+K592100+pSB1C3] C<sup>r</sup>
+GFP[J23101+B0034+E0040+J61048+pSB1C3] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+[pSB6A1]
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A<sup>r</sup>
+<b>Transform of</b>
+OmpA[OmpA-linker] C<sup>r</sup>
                          </td>
                          <td >
-                             <B>29</B><br><br>
+                             <B>29</B><br><br><b>Electrophoresis of</b>
+OmpA[J23101+B0034+OmpA+pSB3C5]
+<b>Transform of</b>
+GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] A<sup>r</sup>
+<b>PCR of</b>
+BFP[B0034+K592100+J61048+pSB1C3] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
+<b>Electrophoresis of</b>
+BFP[B0034+K592100+J61048+pSB1C3] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
+<b>Digestion of </b>
+Anti-Her 2[Herceptin-own] (EcoRI and PstI)
+Anti-Her 2[Herceptin-E1010] (EcoRI and PstI)
+GBP[GBP] (EcoRI and PstI)
+<b>Ligation of</b>
+[R0010+B0034+pSB1K3] K<sup>r</sup>
+BFP[B0030+K592100+J61048] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] A<sup>r</sup>
+GFP[R0010+B0030+E0040+J61048] A<sup>r</sup>
+<b>Electrophoresis of</b>
+Anti-Her 2[Herceptin-own] (EcoRI and PstI)
+Anti-Her 2[Herceptin-E1010] (EcoRI and PstI)
+GBP[GBP] (EcoRI and PstI)
+[R0010+B0034+pSB1K3] K<sup>r</sup>
+BFP[B0030+K592100+J61048] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] A<sup>r</sup>
+GFP[R0010+B0030+E0040+J61048] A<sup>r</sup>
                          </td>
                          <td>
-                            <B>30</B><br><br>
+                            <B>30</B><br><br><b>Cultivation of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] C<sup>r</sup>
+<b>Ligation of</b>
+GBP[FadL-GBP+pSB1C3]C<sup>r</sup>
+<b>Digestion of </b>
+Anti-Her 2[Herceptin own]  (NcoI and PstI)
+[pSB1A3]  (EcoRI and PstI)
+[pSB1C3]  (EcoRI and PstI)
+<b>Cultivation of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
+[J23101+B0034] K<sup>r</sup>
+<b>Digestion of </b>
+scFv-B[scFv-B] K<sup>r</sup> (NcoI and PstI)
+scFv-C[scFv-C] K<sup>r</sup> (NcoI and PstI)
+OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
+<b>Electrophoresis of</b>
+Anti-Her 2[Herceptin own]   (NcoI and PstI)
+[pSB1A3]  (EcoRI and PstI)
+[pSB1C3]  (EcoRI and PstI)
+<b>Plasmid purification of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
+[J231-1+B0034] K<sup>r</sup>
+<b>Digestion of </b>
+scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup> (EcoRI and SpeI)
+[J231-1+B0034] K<sup>r</sup> (EcoRI and SpeI)
+Anti-Her 2[Herceptin-Own] (NcoI and PstI)
                          </td>
                          <td>
-                             <B>31</B><br><br>
+                             <B>31</B><br><br><b>Electrophoresis of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup> (EcoRI and SpeI)
+[J231-1+B0034] K<sup>r</sup> (EcoRI and SpeI)
+Anti-Her 2[Herceptin-Own] (NcoI and PstI)
+<b>Ligation of</b>
+Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] A<sup>r</sup>
+<b>Transform of</b>
+Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] A<sup>r</sup>
+<b>Electrophoresis of</b>
+[pSB1C3] (XbaI and PstI)
+OmpA[OmpA] C<sup>r</sup> (EcoRI and PstI)
+scFv-B[scFv-B] K<sup>r</sup> (NcoI and PstI)
+scFv-C[scFv-C] K<sup>r</sup> (NcoI and PstI)
                          </td>
 						<td >
@@ Line 1,230: / Line 2,438: @@
 					<td >
                              <B>1</B><br><br>
-							PCR<br>
+							<b>Digestion of </b>
-							transform
+Anti-Her 2[Herceptin-Web] (NcoI and PstI)
+<b>Electrophoresis of</b>
+Anti-Her 2[Herceptin-Web] (NcoI and PstI)
                          </td></tr>
                      <tr>
                          <td>
-                             <B>2</B><br><br>
+                             <B>2</B><br><br><b>Electrophoresis of</b>
+[pSB1C3] (XbaI and PstI)
+OmpA[OmpA] (EcoRI and PstI)
+scFv-B[scFv-B] K<sup>r</sup> (NcoI and PstI)
+scFv-C[scFv-C] K<sup>r</sup> (NcoI and PstI)
+OmpA[OmpA] (NcoI and PstI)
+<b>Digestion of </b>
+BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and NcoI)
                          </td>
                          <td>
-                            <B>3</B><br><br>
+                            <B>3</B><br><br><b>Transform of</b>
+[R0010+B0034+pSB1K3] K<sup>r</sup>
+GBP[FadL-GBP+pSB1C3] C<sup>r</sup>
+BFP[B0030+K592100+J61048] C<sup>r</sup>
+<b>Digestion of </b>
+Anti-Her 2[Herceptin-Web] (NcoI and PstI)
+<b>Ligation of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
                          </td>
 						<td >
-                            <B>4</B><br><br>
+                            <B>4</B><br><br><b>Digestion of </b>
+BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and NcoI)
+<b>PCR of</b>
+[R0010+B0034+pSB1K3] K<sup>r</sup>
+BFP[B0030+K592100+J61048] C<sup>r</sup>
+<b>Transform of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
                          </td>
                          <td>
-                            <B>5</B><br><br>
+                            <B>5</B><br><br><b>Digestion of </b>
+[pSB6A1] (EcoRI and PstI)
+[pSB1C3] (EcoRI and PstI)
+<b>PCR of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
+<b>Electrophoresis of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
+<b>Cultivation of</b>
+BFP[B0030+K592100+J61048] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
+<b>PCR of</b>
+GBP[FadL-GBP] C<sup>r</sup>
+BFP[OmpA+K592100] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C] A<sup>r</sup>
+scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
+scFv-B[scFv-B+oSB1C3] C<sup>r</sup>
+<b>Digestion of </b>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] C<sup>r</sup> (EcoRI and PstI)
+<b>Transform of</b>
+GBP[J23101+B0034+FadL-GBP]C<sup>r</sup>
+scFv-B[scFv-B+pSB1C3] C<sup>r</sup>
+scFv-C[scFv-C+pSB1C3] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C<sup>r</sup>
+<b>Gel extraction of</b>
+[pSB6A1] (EcoRI and PstI)
+[OmpA] (NcoI and PstI)
+[pSB1C3] (EcoRI and PstI)
                          </td>
                          <td>
-                             <B>6</B><br><br>
+                             <B>6</B><br><br><b>Cultivation of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
+<b>Ligation of</b>
+Anti-Her 2[OmpA+Herceptin-Web] C<sup>r</sup>
+Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
+scFv-C[OmpA+scFv-C] C<sup>r</sup>
+scFv-B[OmpA+scFv-B] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
+<b>Transform of</b>
+Anti-Her 2[OmpA+Herceptin-Web] C<sup>r</sup>
+Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
+scFv-C[OmpA+scFv-C] C<sup>r</sup>
+scFv-B[OmpA+scFv-B] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
+[pSB1C3]
+[pSB1A3]
+[pSB1K3]
+<b>Plasmid purification of</b>
+BFP[B0030+K592100+J61048]
+<b>Digestion of </b>
+BFP[B0030+K592100+J61048]  (EcoRI and PstI)
+<b>Cultivation of</b>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
+<b>Ligation of</b>
+[R0010+B0034] K<sup>r</sup>
+GBP[GBP[J23101+B0034+FadL-GBP]+pSB1C3] C<sup>r</sup>
+<b>Digestion of </b>
+[pSB1C3] (XbaI and PstI)
+<b>Electrophoresis of</b>
+GBP[J23101+B0034+FadL-GBP] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] C<sup>r</sup>
+scFv-C[scFv-C] C<sup>r</sup>
+scFv-B[scFv-B] C<sup>r</sup>
+<b>Plasmid purification of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
+<b>Digestion of </b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and PstI)
+<b>Ligation of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
+<b>Electrophoresis of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
+[B0034]
+[pSB1C3]
+<b>Cultivation of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
+<b>Transform of</b>
+GBP[J23101+B0034+FadL-GBP] A<sup>r</sup>
+<b>Electrophoresis of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] C<sup>r</sup>
+[pSB1C3] (XbaI and PstI)
+[pSB6A1] (XbaI and PstI)
+[pSB1C3] (XbaI and PstI)
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
+<b>Cultivation of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] C<sup>r</sup>
+GFP[J23101+B0034+E0040+J61048] A<sup>r</sup>
+<b>SDS-page of</b>
+GFP[J23101+B0034+E0040+J61048] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] A<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] A<sup>r</sup>
+[pSB6A1]
+GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] A<sup>r</sup>
+<b>Digestion of </b>
+[pSB1C3] (XbaI and PstI)
+Anti-Her 2[Herceptin-Web] (NcoI and PstI)
+Anti-Her 2[Herceptin-Own] (NcoI and PstI)
+<b>Ligation of</b>
+[pSB1C3] (XbaI and PstI)
+Anti-Her 2[Herceptin-Web] C<sup>r</sup>
+Anti-Her 2[Herceptin-Own] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
                          </td>
 						<td>
-                             <B>7</B><br><br>
+                             <B>7</B><br><br><b>Plasmid purification of</b>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
+<b>Digestion of </b>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
+<b>PCR of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
+<b>Electrophoresis of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and PstI)
+<b>Cultivation of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
+<b>Transform of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
+<b>Plasmid purification of</b>
+GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
+<b>Digestion of </b>
+GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup> (EcoRI and PstI)
+<b>PCR of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
+<b>Electrophoresis of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C] C<sup>r</sup>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
+OmpA[OmpA] (NcoI and PstI)
+[pSB1C3] (EcoRI and PstI)
+<b>Ligation of</b>
+scFv-C[OmpA+scFv-C] C<sup>r</sup>
+scFv-B[OmpA+scFv-B] C<sup>r</sup>
+Anti-Her 2[Herceptin-Own] C<sup>r</sup>
+Anti-Her 2[Herceptin-Web] C<sup>r</sup>
+Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
+Anti-Her 2[OmpA+Herceptin-Web] C
+<b>Electrophoresis of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and PstI)
+Anti-Her 2[Herceptin-Web] (NcoI and PstI)
+Anti-Her 2[Herceptin-Own] (NcoI and PstI)
+<b>Transform of</b>
+scFv-C[OmpA+scFv-C] C<sup>r</sup>
+scFv-B[OmpA+scFv-B] C<sup>r</sup>
+Anti-Her 2[Herceptin-Own] C<sup>r</sup>
+Anti-Her 2[Herceptin-Web] C<sup>r</sup>
+Anti-Her 2[OmpA+Herceptin-Own] C<sup>r</sup>
+Anti-Her 2[OmpA+Herceptin-Web] C
+BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
                          </td> <td >
-                            <B>8</B><br><br>
+                            <B>8</B><br><br><b>Plasmid purification of</b>
+scFv-C[J23101+OmpA+scFv-C] C<sup>r</sup>
+scFv-B[J23101+OmpA+scFv-B] C<sup>r</sup>
+<b>Digestion of </b>
+scFv-C[J23101+OmpA+scFv-C] C<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+OmpA+scFv-B] C<sup>r</sup> (EcoRI and PstI)
+<b>PCR of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
+<b>Cultivation of</b>
+[pSB1K3]
+[pSB1C3]
+[pSB1A3]
+<b>PCR of</b>
+BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
+<b>Transform of</b>
+scFv-C[OmpA+scFv-C] C<sup>r</sup>
+<b>Electrophoresis of</b>
+BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
+scFv-C[J23101+OmpA+scFv-C] C<sup>r</sup> (EcoRI and PstI)
+scFv-B[J23101+OmpA+scFv-B] C<sup>r</sup> (EcoRI and PstI)
+[pSB1C3] (EcoRI and PstI)
+<b>Digestion of </b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup> (EcoRI and SpeI)
+OmpA[OmpA] C<sup>r</sup> NcoI and PstI
+OmpA[OmpA+K592100] C<sup>r</sup> NcoI and PstI
+<b>Ligation of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C<sup>r</sup>
+<b>Digestion of </b>
+Anti-Her 2[Herceptin-Own] (EcoRI and PstI)
+Anti-Her 2[Herceptin-Web] (EcoRI and PstI)
+<b>Electrophoresis of</b>
+BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
                          </td>
                      </tr>
@@ Line 1,259: / Line 2,729: @@
                          <td>
-                            <B>8</B><br><br>
+                            <B>9</B><br><br><b>Cultivation of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup>
+<b>Plasmid purification of</b>
+[pSB1C3]
+[pSB1K3]
+[pSB1A3]
+<b>Digestion of </b>
+[pSB1C3] (EcoRI and PstI)
+[pSB1K3] (EcoRI and PstI)
+[pSB1A3] (EcoRI and PstI)
+<b>Transform of</b>
+Anti-Her 2[Herceptin-Own] C<sup>r</sup>
+Anti-Her 2[Herceptin-Web] C<sup>r</sup>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C<sup>r</sup>
+[R0010+B0034] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100] C<sup>r</sup>
+<b>Electrophoresis of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] C<sup>r</sup> (EcoRI and SpeI)
+OmpA[OmpA] C<sup>r</sup> (NcoI and PstI)
+<b>Digestion of </b>
+[pSB1A3] (EcoRI and PstI)
+[pSB1K3] (EcoRI and PstI)
+[pSB1C3] (EcoRI and PstI)
+RFP[B0030+E1010+J61048] C<sup>r</sup> (XbaI and PstI)
+<b>Electrophoresis of</b>
+scFv-C[OmpA+scFv-C] K<sup>r</sup>
+<b>PCR of</b>
+GBP[GBp+pSB1C3] C<sup>r</sup>
+<b>Digestion of </b>
+BFP[B0030+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
                          </td>
                          <td>
-                            <B>10</B><br><br>
+                            <B>10</B><br><br><b>Electrophoresis of</b>
+GBP[GBp+pSB1C3] C<sup>r</sup>
+[pSB1A3] (EcoRI and PstI)
+[pSB1K3] (EcoRI and PstI)
+[pSB1C3] (EcoRI and PstI)
+<b>Ligation of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web+B0030+E1010+J61048] K<sup>r</sup>
+<b>Cultivation of</b>
+[R0010+B0034] C<sup>r</sup>
+BFP[B0030+K592100+J61048] C<sup>r</sup>
+scFv-C[OmpA+scFv-C] K<sup>r</sup>
+<b>Digestion of </b>
+[pSB1C3] (EcoRI and Pstl)
+<b>Transform of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] K<sup>r</sup>
+<b>Gel extraction of</b>
+[pSB1C3] (EcoRI and PstI)
+<b>PCR of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web]
+Anti-Her 2[Herceptin-Web]
+Anti-Her 2[Herceptin-Own]
                          </td>
 						<td >
-                            <B>11</B><br><br>
+                            <B>11</B><br><br><b>Plasmid purification of</b>
+[R0010+B0034] C<sup>r</sup>
+scFv-C[OmpA+scFv-C] K<sup>r</sup>
+BFP[OmpA+K592100] C<sup>r</sup>
+<b>Gel extraction of</b>
+[pSB1C3] (EcoRI and PstI)
+<b><b><b>Digestion of </b> </b></b>
+[R0010+B0034] C<sup>r</sup> (EcoRI and SpeI)
+scFv-C[OmpA+scFv-C] K<sup>r</sup> (EcoRI and PstI)
+BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and PstI)
+GBP[J23101+B0034+FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
+<b>Ligation of</b>
+Anti-Her 2[Herceptin-Web] C<sup>r</sup>
+<b>PCR of</b>
+scFv-B[OmpA+scFv-B] K<sup>r</sup>
+<b>Electrophoresis of</b>
+[R0010+B0034] C<sup>r</sup> (EcoRI and SpeI)
+scFv-C[OmpA+scFv-C] K<sup>r</sup> (EcoRI and PstI)
+BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and PstI)
+GBP[J23101+B0034+FadL-GBP] C<sup>r</sup> (EcoRI and SpeI)
+<b>Ligation of</b>
+GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Electrophoresis of</b>
+scFv-B[OmpA+scFv-B] K<sup>r</sup>
+<b>PCR of</b>
+scFv-C[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
+<b>Transform of</b>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
                          </td>
                          <td>
-                             <B>12</B><br><br>
+                             <B>12</B><br><br><b>Electrophoresis of</b>
+scFv-B[OmpA+scFv-B] K<sup>r</sup>
+<b>Transform of</b>
+Anti-Her 2[Herceptin-Web] C<sup>r</sup>
+Anti-Her 2[Herceptin-Own] C<sup>r</sup>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] A<sup>r</sup>
+<b>PCR of</b>
+scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Digestion of </b>
+[pSB1C3] (EcoRI and PstI)
+<b>Electrophoresis of</b>
+scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td>
                          <td>
-                             <B>13</B><br><br>
+                             <B>13</B><br><br><b>Transform of</b>
+GBP[J231010+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Cultivation of</b>
+[pSB1C3]
+scFv-B[OmpA-scFv-B] K<sup>r</sup>
+<b>Plasmid purification of</b>
+[pSB1C3]
+scFv-B[OmpA-scFv-B] K<sup>r</sup>
+<b>Digestion of </b>
+[pSB1C3] (EcoRI and PstI)
+scFv-B[OmpA-scFv-B] K<sup>r</sup> (EcoRI and PstI)
+<b>Electrophoresis of</b>
+[pSB1C3] (EcoRI and PstI)
+<b>Ligation of</b>
+OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Electrophoresis of</b>
+[pSB1C3] (EcoRI and PstI)
+scFv-B[OmpA-scFv-B] K<sup>r</sup> (EcoRI and PstI)
                          </td>
 						<td >
-                             <B>14</B><br><br>
+                             <B>14</B><br><br><b>Transform of</b>
+OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td><td >
-                             <B>15</B><br><br>
+                             <B>15</B><br><br><b>PCR of</b>
+OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Ligation of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
+BFP[J23101+B0034+OmpA+K592100+pSB3C5] C<sup>r</sup>
+<b>PCR of</b>
+GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
+<b>Transform of</b>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
+<b>Electrophoresis of</b>
+GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
+<b>Electrophoresis of</b>
+GBP[FadL-GBP] (EcoRI and PstI)
                          </td>
                      </tr>
@@ Line 1,282: / Line 2,925: @@
                          <td>
-                             <B>16</B><br><br>
+                             <B>16</B><br><br><b>PCR of</b>
+GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] K<sup>r</sup>
+<b>Cultivation of</b>
+OmpA[R0010+B0034+OmpA]
+<b>Electrophoresis of</b>
+GBP[R0010+B0034+FadL-GBP+pSB1A3] K<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] K<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] K<sup>r</sup>
                          </td>
                          <td>
-                             <B>17</B><br><br>
+                             <B>17</B><br><br><b>Ligation of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>Transform of</b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td>
 						<td >
-                             <B>18</B><br><br>
+                             <B>18</B><br><br><b>Plasmid purification of</b>
+OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+<b>Digestion of </b>
+BFP[OmpA+K592100] C<sup>r</sup> (EcoRI and NcoI)
+Anti-Her 2[Herceptin-Own] (NcoI and SpeI)
+Anti-Her 2[Herceptin-Web] (NcoI and SpeI)
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup> (EcoRI and SpeI)
+BFP[B0030+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
+OmpA[R0010+B0034+OmpA+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup> (EcoRI and PstI)
+<b>Ligation of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+<b>Transform of</b>
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+pSB1A3] A<sup>r</sup>
+GBP[J23101+B0034+fadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
+OmpA[R0010+B0034+OmpA+pSB1C3] C<sup>r</sup>
+GBP[R0010+B0034+FadL-GBP+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048]
+<b>Ligation of</b>
+scFv-B[R0010+B0034+OmpA+scFv-B]
+scFv-C[R0010+B0034+OmpA+scFv-C]
+Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own]
+Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web]
+GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048]
+<b>PCR of</b>
+Anti-Her 2[Herceptin-Web]
+scFv-B[J23101+B0034+OmpA+scFv-B]
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
+Anti-Her 2[Herceptin-Own]
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
                          </td>
                          <td>
-                             <B>19</B><br><br>
+                             <B>19</B><br><br><b>Electrophoresis of</b>
+Anti-Her 2[Herceptin-Web]
+scFv-B[J23101+B0034+OmpA+scFv-B]
+scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] C<sup>r</sup>
+Anti-Her 2[Herceptin-Own]
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+scFv-C[OmpA+scFv-C]
+Anti-Her 2[Herceptin-Own] (NcoI and SpeI)
+Anti-Her 2[Herceptin-Web] (NcoI and SpeI)
+Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] A<sup>r</sup> (EcoRI and SpeI)
+BFP[B0030+K592100+J61048] C<sup>r</sup> (XbaI and PstI)
+<b>Transform of</b>
+GBP[R0010+B034+FadL-GBP] A<sup>r</sup>
+GBP[J23101+B0034+FadL-GBP] A<sup>r</sup>
+GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] C<sup>r</sup>
                          </td>
                          <td>
-                             <B>20</B><br><br>
+                             <B>20</B><br><br><b>PCR of</b>
+GBP[FadL-GBP] K<sup>r</sup>
+OmpA[OmpA] C<sup>r</sup>
+<b>Gel extraction of</b>
+OmpA[OmpA] (EcoRI and SpeI)
+<b>Plasmid purification of</b>
+Anti-Her 2[Herceptin-Own] C<sup>r</sup>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup>
+<b>PCR of</b>
+GBP[FadL-GBP] K<sup>r</sup>
+OmpA[R0010+B0034+OmpA+pSB1C3] C<sup>r</sup>
+GBP[R0010+B0034+GBP+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
+GBP[R0010+B0034+GBP+B0030+E0040+J61048]
+scFv-B[R0010+B0034+OmpA+scFv-B]
+scFv-C[R0010+B0034+OmpA+scFv-C]
+Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own]
+Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web]
+<b>Digestion of </b>
+scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] A<sup>r</sup> (EcoRI and PstI)
+Anti-Her 2[Herceptin-Own] C<sup>r</sup>
+<b>Electrophoresis of</b>
+OmpA[R0010+B0034+OmpA+pSB1C3] C<sup>r</sup>
+GBP[R0010+B0034+GBP+pSB1C3] C<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] K<sup>r</sup>
+GBP[R0010+B0034+GBP+B0030+E0040+J61048]
+<b>Ligation of</b>
+scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] K<sup>r</sup>
+scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] K<sup>r</sup>
+Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] K<sup>r</sup>
+Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] K<sup>r</sup>
+GBP[R0010+B0034+GBP+B0030+E0040+J61048pSB1A3] A<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
+scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] K<sup>r</sup>
+Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] K<sup>r</sup>
                          </td>
 						<td >