Difference between revisions of "Team:H4Z-Hangzhou"

 
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      <div style="background-image: url('https://static.igem.org/mediawiki/2015/1/1f/Bottles.jpg');" class="fullscreen-image"></div>
 
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<li>
 
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<!-- Service -->
 
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<h3 class="color">I design</h3>
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<h3 class="color">background</h3>
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<p>Design often necessitates considering the aesthetic and functional dimensions of both the design object and process. It involves research, thought, modeling, interactive adjustment, and re-design. Diverse kinds of objects may be designed, including clothing, graphical user interfaces and corporate identities.</p>
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<p>Our IGEM research project involves study of the autophagy phenomenon by using the GFP to reveal it more clearly. The Autophagy is a biological process which acts like a recycle factory. Like you put a pop can into the trash bin and then reproduce by the factory, the procedure of autophagy just catch the aging, denaturing, damaged organelles and send them into the lysosome which degrades the organelles. </p>
 
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<!-- Service -->
 
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<h3 class="color">I code</h3>
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<h3 class="color">Health</h3>
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<p>Coding often necessitates considering the aesthetic and functional dimensions of both the design object and process. It involves research, thought, modeling, interactive adjustment, and re-design. Diverse kinds of objects may be designed, including clothing, graphical user interfaces and corporate identities.</p>
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<p>Autophagy is developed as a way to address the disease and maintain the health of human body. With the degradation of the denaturing cellular, our cells can have the ability to defend themselves without other medical treatment.</p>
 
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<!-- Service -->
 
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<h3 class="color">I make illustrations</h3>
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<h3 class="color">Anti-aging</h3>
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<p>Illustration often necessitates considering the aesthetic and functional dimensions of both the design object and process. It involves research, thought, modeling, interactive adjustment, and re-design. Diverse kinds of objects may be designed, including clothing, graphical user interfaces and corporate identities.</p>
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<p>Autophagy is also capable to keep us young and strong. It is better than the cosmetic we used which may cause other problem.</p>
 
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<li>
 
<!-- Service -->
 
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<h3 class="color">I take beautiful pictures</h3>
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<h3 class="color">Fight with cancer.</h3>
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<p>Photography often necessitates considering the aesthetic and functional dimensions of both the design object and process. It involves research, thought, modeling, interactive adjustment, and re-design. Diverse kinds of objects may be designed, including clothing, graphical user interfaces and corporate identities.</p>
+
<p>Autophagy gets the potential impact to fight with cancer. However it still get some negative effect. The reaction taken place in the lysosome can consciously can unconsciously help the cancer cell to grow. It may be an efficient assist of deteriorating the denaturation of tumor cell. After all, it still play a paramount significance in the future genetic study.</p>
 
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<h3>Environmental Impact</h3>
 
<h3>Environmental Impact</h3>
 
<p>Since our subject is a fundamental work in this field, all the processes were finished in the laboratory. It did not bring any negative effects to our environment.</p>
 
<p>Since our subject is a fundamental work in this field, all the processes were finished in the laboratory. It did not bring any negative effects to our environment.</p>
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<h3>Safety</h3>
 
<h3>Safety</h3>
 
<p>The experiment itself is totally safe. Although we met some trouble during the operation of experiment, fortunately, all the troubles did not cause any hurt to teammates or others.</p>
 
<p>The experiment itself is totally safe. Although we met some trouble during the operation of experiment, fortunately, all the troubles did not cause any hurt to teammates or others.</p>
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<h3>Education</h3>
 
<h3>Education</h3>
 
<p>In order to make the public have deeper comprehending of genetic modification, we did a lecture and a questionnaire of people about GM food. The reason why we used the GM food as the topic but not the subject of our experiment was that our subject is too professional for normal people to understand.</p>
 
<p>In order to make the public have deeper comprehending of genetic modification, we did a lecture and a questionnaire of people about GM food. The reason why we used the GM food as the topic but not the subject of our experiment was that our subject is too professional for normal people to understand.</p>
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<h3>Evaluation</h3>
 
<h3>Evaluation</h3>
 
<p>We summarized and processed our statistics. It is not surprised that the students accept GM food in a much larger extent than that in general public.</p>
 
<p>We summarized and processed our statistics. It is not surprised that the students accept GM food in a much larger extent than that in general public.</p>
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<b>Illustration</b></span>
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<b>Team Leader and Experiment Part Supervisor of iGEM 2015</b></span>
 
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<b>Prime Instructor of Experiment of iGEM 2015</b></span>
 
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<span>Hongchang Fu<br>
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<span>Yuge Zhang<br>
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<b>English Consultant and Basic Knowledge Lecturer</b></span>
 
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<span>yaxin deng<br>
 
<span>yaxin deng<br>
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<b>Prime Designer and Art Director</b></span>
 
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<div class="copyrights">Collect from <a href="http://www.cssmoban.com/" >免费模板</a></div>
+
 
<!-- About me introduction -->
 
<!-- About me introduction -->
 
<div class="row">
 
<div class="row">
 
<div class="ten columns offset-by-one margin-bottom">
 
<div class="ten columns offset-by-one margin-bottom">
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<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Fusce lectus elit, tincidunt nec turpis sed, accumsan iaculis ipsum. Nulla at augue auctor, tristique erat in, ultricies nunc. Mauris eget metus leo. Ut in mi lacinia, mattis nisl non, ultrices risus. Vestibulum aliquet aliquam ipsum ut ullamcorper. Pellentesque fringilla, massa vel rutrum consequat, nulla velit fermentum dolor, sed scelerisque mauris mauris non lectus.</p>
+
<p>Autophagy gets the potential impact to fight with cancer. However it still get some negative effect. The reaction taken place in the lysosome can consciously can unconsciously help the cancer cell to grow. It may be an efficient assist of deteriorating the denaturation of tumor cell. After all, it still play a paramount significance in the future genetic study.
−
<img src="images/placeholder-avatar.jpg" alt="placeholder-avatar" class="image-center image-circle margin-bottom">
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<h3>I love photography</h3>
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−
<p>Etiam dictum gravida nibh at accum san. Quisque aliquam risus bibendum diam elementum, et tempus enim suscipit. Duis sit amet euismod.</p>
+
<p>Competent E.coli cell preparation</p>
 +
                                <p>Transform pIREs2-GFP-LC3 into Competent E.coli cell </p>
 
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<h3>I'm a big music fan</h3>
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−
<p>Etiam dictum gravida nibh at accum san. Quisque aliquam risus bibendum diam elementum, et tempus enim suscipit. Duis sit amet euismod.</p>
+
<p>Restriction enzyme digestion: get GFP-LC3 gene fragment from pIREs2-GFP-LC3 </p>
 +
                                <p>Purification of gene fragment GFP-LC3 </p>
 
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<h3>Illustrating is my passion</h3>
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<p>Etiam dictum gravida nibh at accum san. Quisque aliquam risus bibendum diam elementum, et tempus enim suscipit. Duis sit amet euismod.</p>
+
<p>Ligation: GFP-LC3+PSB1C3</p>
 +
                                <p>Transform pSB1C3-GFP-LC3 into Competent E.coli cell </p>
 
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<h3>I love watching comedies</h3>
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−
<p>Etiam dictum gravida nibh at accum san. Quisque aliquam risus bibendum diam elementum, et tempus enim suscipit. Duis sit amet euismod.</p>
+
<p>Mini-prep of Plasmids PSB1C3-GFP-LC3</p>
 +
                                <p>Electrophoresis and Sequencing</p>
 
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<a href="#home" class="back-to-top"><i class="icon-angle-up"><img src="https://static.igem.org/mediawiki/2015/f/f7/Toptop.png"></i></a>
 
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Latest revision as of 13:52, 14 September 2015

Focus - Personal Edition

  • iGEM HANGZHOU

    More accurate and distinct observation? Special fluorescent protein marker.

    LEARN MORE

  • Cancer? Autophagy?

    Find a link between them.

    Experience more

  • Human Practice

    You will see our excellent work.

  • The Truth Is Going to be revealed below.

    Experience more

Project

This is what We do

  • background

    Our IGEM research project involves study of the autophagy phenomenon by using the GFP to reveal it more clearly. The Autophagy is a biological process which acts like a recycle factory. Like you put a pop can into the trash bin and then reproduce by the factory, the procedure of autophagy just catch the aging, denaturing, damaged organelles and send them into the lysosome which degrades the organelles.

  • Health

    Autophagy is developed as a way to address the disease and maintain the health of human body. With the degradation of the denaturing cellular, our cells can have the ability to defend themselves without other medical treatment.

  • Anti-aging

    Autophagy is also capable to keep us young and strong. It is better than the cosmetic we used which may cause other problem.

  • Fight with cancer.

    Autophagy gets the potential impact to fight with cancer. However it still get some negative effect. The reaction taken place in the lysosome can consciously can unconsciously help the cancer cell to grow. It may be an efficient assist of deteriorating the denaturation of tumor cell. After all, it still play a paramount significance in the future genetic study.

Human Practice

How We do it

Environmental Impact

Since our subject is a fundamental work in this field, all the processes were finished in the laboratory. It did not bring any negative effects to our environment.

Safety

The experiment itself is totally safe. Although we met some trouble during the operation of experiment, fortunately, all the troubles did not cause any hurt to teammates or others.

Education

In order to make the public have deeper comprehending of genetic modification, we did a lecture and a questionnaire of people about GM food. The reason why we used the GM food as the topic but not the subject of our experiment was that our subject is too professional for normal people to understand.

Evaluation

We summarized and processed our statistics. It is not surprised that the students accept GM food in a much larger extent than that in general public.

Portfolio

  • E.coli&LC3
  • GFP-LC3
  • pSB1C3 as Vector
  • Fluorescent Protein Marker
  • Autophagosomes

Project details

HOW WE DO IT

Autophagy gets the potential impact to fight with cancer. However it still get some negative effect. The reaction taken place in the lysosome can consciously can unconsciously help the cancer cell to grow. It may be an efficient assist of deteriorating the denaturation of tumor cell. After all, it still play a paramount significance in the future genetic study.

Competent E.coli cell preparation

Transform pIREs2-GFP-LC3 into Competent E.coli cell

Restriction enzyme digestion: get GFP-LC3 gene fragment from pIREs2-GFP-LC3

Purification of gene fragment GFP-LC3

Ligation: GFP-LC3+PSB1C3

Transform pSB1C3-GFP-LC3 into Competent E.coli cell

Mini-prep of Plasmids PSB1C3-GFP-LC3

Electrophoresis and Sequencing

Timeline

Our work experiences

23th Dec

iGEM Intro and Potential Member Selection.

27th Jan

Brainstorming and Presentation.

5th Mar

Final Membership Confirmed.

13th Apr

ZJU Lab Permission Registration.

17th May

Discussion Conference with ZJU & Peking University.

12th Jun

PCR Operation Skills Training and Preparation for Experiment.

From Jun

Training Every Single Two weeks and Lab Work.

24th Aug

Final Stage of Our Experiment.

Sponsors

sponsors

CONTACT US

No.436, No.6 Road, Xiasha

Hangzhou,Zhejiang, China

Phone:+8613957137482

Mail:ricardo937@outlook.com

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