Difference between revisions of "Team:H4Z-Hangzhou"

 
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{{H4Z-Hangzhou}}
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<html>
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<html>
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<h2> Welcome to iGEM 2015! </h2>
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<!--[if IE 7]><html lang="en" class="ie7"><![endif]-->
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<!--[if IE 8]><html lang="en" class="ie8"><![endif]-->
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<!--[if IE 9]><html lang="en" class="ie9"><![endif]-->
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<!--[if (gt IE 9)|!(IE)]><html lang="en"><![<math>Insert formula here</math>endif]-->
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<!--[if !IE]><html lang="en"><![endif]-->
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<head>
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<h4>Before you start: </h4>
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        <style>#content{width:100%}</style>
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<p> Please read the following pages:</p>
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<ul>
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<li>  <a href="https://2015.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2015.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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</ul>
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<div class="highlightBox">
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        <meta charset="utf-8">
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<h4> Styling your wiki </h4>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<script type="text/javascript" src="https://2015.igem.org/Template:H4Z-Hangzhou/js/lib/modernizr?action=raw&ctype=text/javascript"></script>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<script type="text/javascript" src="https://2015.igem.org/Template:H4Z-Hangzhou/js/lib/pace?action=raw&ctype=text/javascript"></script>
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</div>
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<script>(function(w){var dpr=((w.devicePixelRatio===undefined)?1:w.devicePixelRatio);if(!!w.navigator.standalone){var r=new XMLHttpRequest();r.open('GET','/retinaimages.php?devicePixelRatio='+dpr,false);r.send()}else{document.cookie='devicePixelRatio='+dpr+'; path=/'}})(window)</script>
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<noscript><style id="devicePixelRatio" media="only screen and (-moz-min-device-pixel-ratio: 2), only screen and (-o-min-device-pixel-ratio: 2/1), only screen and (-webkit-min-device-pixel-ratio: 2), only screen and (min-device-pixel-ratio: 2)">html{background-image:url("/retinaimages.php?devicePixelRatio=2")}</style></noscript>
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<title>Focus - Personal Edition</title>
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<meta name="robots" content="index, nofollow">
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<meta name="description" content="Your website description here">
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<meta name="keywords" content="your, website, keywords, here">
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<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
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<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:H4Z-Hangzhou/css/lib/font-awesomexmin?action=raw&ctype=text/css" />
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<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:H4Z-Hangzhou/css/lib/magnific-popup?action=raw&ctype=text/css" />
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<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:H4Z-Hangzhou/css/lib/lite?action=raw&ctype=text/css" />
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<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:H4Z-Hangzhou/css/style?action=raw&ctype=text/css" />
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<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Template:H4Z-Hangzhou/css/blue?action=raw&ctype=text/css" />
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<script src="http://html5shim.googlecode.com/svn/trunk/html5.js"></script>
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<![endif]-->
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<link rel="shortcut icon" href="images/favicon/favicon.ico">
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</head>
  
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<h4> Editing your wiki </h4>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p> <a href="https://2015.igem.org/wiki/index.php?title=Team:H4Z-Hangzhou&action=edit"> Click here to edit this page! </a></p>
 
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<p>See tips on how to edit your wiki on the <a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation</a> page.</p>
 
  
  
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<h4>Templates </h4>
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<!-- Menu -->
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<p> This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the
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<nav class="menu" id="theMenu">
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<a href="https://2015.igem.org/TemplatesforTeams_Code_Documentation">Template Documentation </a> page.</p>  
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<div class="menu-wrap">
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<a href="#home"><img src="https://static.igem.org/mediawiki/2015/e/e2/Igemlogo.png" longdesc="https://static.igem.org/mediawiki/2015/e/e2/Igemlogo.png"></a>
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<!--<h1 class="logo"><a href="#home">Focus</a></h1>-->
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<!--<i class="icon-remove menu-close"></i>-->
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<a href="#home">Home</a>
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<a href="#services">OVERVIEW</a>
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<a href="#process">Human Practice</a>
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<a href="#portfolio">Profile</a>
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<a href="#about-me">PROJECT</a>
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<a href="#"><i class="icon-facebook"></i></a>
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<a href="#"><i class="icon-twitter"></i></a>
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<a href="#"><i class="icon-dribbble"></i></a>
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<a href="#"><i class="icon-envelope"></i></a>
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</div>
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<!-- Menu button -->
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<div id="menuToggle"><img src="https://static.igem.org/mediawiki/2015/5/5f/Tuding.png"></i></div>
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</nav>
  
  
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<h4>Tips</h4>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<ul>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and how you plan to do this.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2015.igem.org/Calendar_of_Events">iGEM 2015 calendar</a> </li>
 
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<li>Have lots of fun! </li>
 
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</ul>
 
  
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<h4>Inspiration</h4>
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<div class="right-menu noprint" id="menubar"></div><!-- end right menubar -->
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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<h4> Uploading pictures and files </h4>
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<!-- Fullscreen slider -->
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<p> You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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<div id="home"></div>
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<div id="slides">
 +
<ul class="slides-container">
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<li>
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<!-- Slide -->
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<div style="background-image: url('https://static.igem.org/mediawiki/2015/d/d0/Main.jpg');" class="fullscreen-image"></div>
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 +
                                <div class="header-center fade-in">
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                                    <h1 class="uppercase white">iGEM HANGZHOU</h1>
 +
            <p class="uppercase white">More accurate and distinct observation? Special fluorescent protein marker.</p>
 +
            <p><a href="#introduction" class="button white-border">LEARN MORE</a></p>
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</div>
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</li>
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<li>
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 +
<!-- Slide -->
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<div style="background-image: url('https://static.igem.org/mediawiki/2015/9/95/Fire.jpg');" class="fullscreen-image"></div>
 +
<div class="header-center fade-in">
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<h1 class="fittext white">Cancer? Autophagy?</h1>
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<p class="uppercase white">Find a link between them.</p>
 +
<p><a href="#introduction" class="button white-border">Experience more</a></p>
 +
</div>
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</li>
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<li>
 +
                     
 +
<!-- Slide -->
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<div style="background-image: url('https://static.igem.org/mediawiki/2015/c/c5/Ricardohand.jpg');" class="fullscreen-image"></div>
 +
                                <div class="header-center fade-in">
 +
<h1 class="fittext white">Human Practice</h1>
 +
<p class="uppercase white">You will see our excellent work.</p>
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</div>
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</li>
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<li>
 +
                         
 +
<!-- Slide with a logo - or any other image -->
 +
      <div style="background-image: url('https://static.igem.org/mediawiki/2015/1/1f/Bottles.jpg');" class="fullscreen-image"></div>
 +
                              <div class="header-center fade-in">
 +
 +
<p class="uppercase white">The Truth Is Going to be revealed below.</p>
 +
<p><a href="#introduction" class="button white-border">Experience more</a></p>
 +
</div>
 +
</li>
 +
</ul>
 +
<nav class="slides-navigation">
 +
<a href="#" class="prev"><i class="icon-long-arrow-left"></i></a>
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<a href="#" class="next"><i class="icon-long-arrow-right"></i></a>
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</nav>
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<nav class="slides-pagination"><a href="#1" class="">1</a><a href="#2">2</a><a href="#3">3</a><a href="#4" class="current">4</a></nav></div>
  
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<a href="https://2015.igem.org/Special:Upload">CLICK HERE TO UPLOAD FILES</a>
 
  
  
 +
<!-- Introduction -->
 +
<section id="introduction" class="introduction">
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<div class="row">
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<div class="ten columns offset-by-one">
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</div>
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</div>
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</section>
  
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</div></div> <!--These are the closing tags for div id="mainContainer" and div id="contentContainer". The corresponding opening tags appear in the template that is {{included}} at the top of this page.-->
 
  
 +
<!-- Services -->
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<section id="services" class="services">
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 +
<!-- Title -->
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<div class="row">
 +
<div class="ten columns offset-by-one title">
 +
<h1>Project</h1>
 +
<h2>This is what We do</h2>
 +
<hr>
 +
</div>
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</div>
 +
 +
<!-- Content of the services slider -->
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<div class="row">
 +
<div id="services-slider" class="ten columns offset-by-one">
 +
<ul>
 +
<li>
 +
<!-- Service -->
 +
<h3 class="color">background</h3>
 +
<p>Our IGEM research project involves study of the autophagy phenomenon by using the GFP to reveal it more clearly. The Autophagy is a biological process which acts like a recycle factory. Like you put a pop can into the trash bin and then reproduce by the factory, the procedure of autophagy just catch the aging, denaturing, damaged organelles and send them into the lysosome which degrades the organelles. </p>
 +
</li>
 +
<li>
 +
<!-- Service -->
 +
<h3 class="color">Health</h3>
 +
<p>Autophagy is developed as a way to address the disease and maintain the health of human body. With the degradation of the denaturing cellular, our cells can have the ability to defend themselves without other medical treatment.</p>
 +
</li>
 +
<li>
 +
<!-- Service -->
 +
<h3 class="color">Anti-aging</h3>
 +
<p>Autophagy is also capable to keep us young and strong. It is better than the cosmetic we used which may cause other problem.</p>
 +
</li>
 +
<li>
 +
<!-- Service -->
 +
<h3 class="color">Fight with cancer.</h3>
 +
<p>Autophagy gets the potential impact to fight with cancer. However it still get some negative effect. The reaction taken place in the lysosome can consciously can unconsciously help the cancer cell to grow. It may be an efficient assist of deteriorating the denaturation of tumor cell. After all, it still play a paramount significance in the future genetic study.</p>
 +
</li>
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</ul>
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</div>
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</div>
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<!-- Circle controls -->
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<div class="row">
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<div class="services-buttons">
 +
<a data-rel="1" href="javascript:void(0);" class="servicesLink"><img src="https://static.igem.org/mediawiki/2015/9/99/Racket.png"></i></a>
 +
<a data-rel="1" href="javascript:void(0);" class="servicesLink"><img src="https://static.igem.org/mediawiki/2015/9/9f/Xianweijing.png"></i></a>
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<a data-rel="1" href="javascript:void(0);" class="servicesLink"><img src="https://static.igem.org/mediawiki/2015/9/91/Zhuixingpin.png"></i></a>
 +
<a data-rel="1" href="javascript:void(0);" class="servicesLink"><img src="https://static.igem.org/mediawiki/2015/0/05/Fangdajing.png"></i></a>
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</div>
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</div>
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 +
</section>
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 +
 +
<!-- Process -->
 +
<section id="process" class="process">
 +
 +
<!-- Title -->
 +
<div class="row">
 +
<div class="ten columns offset-by-one title">
 +
<h1>Human Practice</h1>
 +
<h2>How We do it</h2>
 +
<hr>
 +
</div>
 +
</div>
 +
 +
<!-- Process steps -->
 +
<div class="row">
 +
 +
<!-- Process step -->
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<div class="desktop-three columns tablet-six columns mobile-six columns">
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<i class="process-icon"><img src="https://static.igem.org/mediawiki/2015/a/aa/Lianjie.png"></i>
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<h3>Environmental Impact</h3>
 +
<p>Since our subject is a fundamental work in this field, all the processes were finished in the laboratory. It did not bring any negative effects to our environment.</p>
 +
</div>
 +
 +
<!-- Process step -->
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<div class="desktop-three columns tablet-six columns mobile-six columns">
 +
<i class="process-icon"><img src="https://static.igem.org/mediawiki/2015/8/8e/Dengpao.png"></i>
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<h3>Safety</h3>
 +
<p>The experiment itself is totally safe. Although we met some trouble during the operation of experiment, fortunately, all the troubles did not cause any hurt to teammates or others.</p>
 +
</div>
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 +
<!-- Process step -->
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<div class="desktop-three columns tablet-six columns mobile-six columns">
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<i class="process-icon"><img src="https://static.igem.org/mediawiki/2015/6/6c/Chilun.png"></i>
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<h3>Education</h3>
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<p>In order to make the public have deeper comprehending of genetic modification, we did a lecture and a questionnaire of people about GM food. The reason why we used the GM food as the topic but not the subject of our experiment was that our subject is too professional for normal people to understand.</p>
 +
</div>
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 +
<!-- Process step -->
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<div class="desktop-three columns tablet-six columns mobile-six columns">
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<i class="process-icon"><img src="https://static.igem.org/mediawiki/2015/b/bc/Banshou.png"></i>
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<h3>Evaluation</h3>
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<p>We summarized and processed our statistics. It is not surprised that the students accept GM food in a much larger extent than that in general public.</p>
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</div>
 +
 +
</div>
 +
</section>
 +
 +
 +
<!-- Portfolio -->
 +
<section id="portfolio" class="portfolio">
 +
 +
<!-- Title and navigation -->
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<div class="row">
 +
<div class="ten columns offset-by-one title">
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<h1>Portfolio</h1>
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 +
<hr>
 +
</div>
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</div>
 +
 +
<!-- Portfolio grid with projects -->
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<div id="portfolio-grid" class="row">
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<!-- Project item -->
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<div class="desktop-four columns tablet-six columns mobile-six columns mix photo project-item">
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<a class="popup" href="https://static.igem.org/mediawiki/2015/2/21/Ricardo.JPG">
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<span class="project-hover">
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<span>Hongchang Fu<br>
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<b>Team Leader and Experiment Part Supervisor of iGEM 2015</b></span>
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</span>
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<img src="https://static.igem.org/mediawiki/2015/2/21/Ricardo.JPG" alt="placeholder-thumbnail">
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</a>
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</div>
 +
 +
                        <!-- Project item -->
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<div class="desktop-four columns tablet-six columns mobile-six columns mix illustration project-item">
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<a class="popup" href="https://static.igem.org/mediawiki/2015/c/c7/Linjimin.JPG">
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<span class="project-hover">
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<span>Jimin Lin<br>
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<b>Prime Instructor of Experiment of iGEM 2015</b></span>
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</span>
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<img src="https://static.igem.org/mediawiki/2015/c/c7/Linjimin.JPG" alt="placeholder-thumbnail">
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</a>
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</div>
 +
 +
<!-- Project item -->
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<div class="desktop-four columns tablet-six columns mobile-six columns mix website project-item">
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<a class="popup" href="https://static.igem.org/mediawiki/2015/e/e4/Zhangyuge.JPG">
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<span class="project-hover">
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<span>Yuge Zhang<br>
 +
<b>English Consultant and Basic Knowledge Lecturer</b></span>
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</span>
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<img src="https://static.igem.org/mediawiki/2015/e/e4/Zhangyuge.JPG" alt="placeholder-thumbnail">
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</a>
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</div>
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 +
 +
 +
<!-- Project item -->
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<div class="desktop-four columns tablet-six columns mobile-six columns mix photo project-item">
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<a class="popup" href="https://static.igem.org/mediawiki/2015/d/d2/Monica.JPG">
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<span class="project-hover">
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<span>yaxin deng<br>
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<b>Prime Designer and Art Director</b></span>
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</span>
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<img src="https://static.igem.org/mediawiki/2015/d/d2/Monica.JPG" alt="placeholder-thumbnail">
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</a>
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</div>
 +
 +
<!-- Project item -->
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<div class="desktop-four columns tablet-six columns mobile-six columns mix website project-item">
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<a class="popup" href="https://static.igem.org/mediawiki/2015/6/69/Victor.JPG">
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<span class="project-hover">
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<span>zhehao shen<br>
 +
<b>Computer Engineer and Website Development Supervisor</b></span>
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</span>
 +
<img src="https://static.igem.org/mediawiki/2015/6/69/Victor.JPG" alt="placeholder-thumbnail">
 +
</a>
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</div>
 +
 +
 
 +
            <!-- Project item -->
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<div class="desktop-four columns tablet-six columns mobile-six columns mix photo project-item">
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<a class="popup" href="https://static.igem.org/mediawiki/2015/0/0b/Binjie.JPG">
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<span class="project-hover">
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<span>binjie sun<br>
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<b>Experiment Operator and Team Coordinator</b></span>
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</span>
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<img src="https://static.igem.org/mediawiki/2015/0/0b/Binjie.JPG" alt="placeholder-thumbnail">
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</a>
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 +
                </div>
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<div class="desktop-four columns tablet-six columns mobile-six columns mix photo project-item">
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<a class="popup" href="https://static.igem.org/mediawiki/2015/0/01/Liangyixiao.JPG">
 +
<span class="project-hover">
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<span>yixiao liang<br>
 +
<b>Lecturer and Experiment Assistant</b></span>
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</span>
 +
<img src="https://static.igem.org/mediawiki/2015/0/01/Liangyixiao.JPG" alt="placeholder-thumbnail">
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</a>
 +
</div>
 +
       
 +
 +
            <!-- Project item -->
 +
<div class="desktop-four columns tablet-six columns mobile-six columns mix photo project-item">
 +
<a class="popup" href="https://static.igem.org/mediawiki/2015/a/a2/Zhangqinru.JPG">
 +
<span class="project-hover">
 +
<span>qinru zhang<br>
 +
<b>Vice Designer</b></span>
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</span>
 +
<img src="https://static.igem.org/mediawiki/2015/a/a2/Zhangqinru.JPG" alt="placeholder-thumbnail">
 +
</a>
 +
</div>
 +
       
 +
       
 +
<!-- Project item -->
 +
<div class="desktop-four columns tablet-six columns mobile-six columns mix photo project-item">
 +
<a class="popup" href="https://static.igem.org/mediawiki/2015/c/c0/Shenchen.JPG">
 +
<span class="project-hover">
 +
<span>chen shen<br>
 +
<b>Human Practice Advisor</b></span>
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</span>
 +
<img src="https://static.igem.org/mediawiki/2015/c/c0/Shenchen.JPG" alt="placeholder-thumbnail">
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</a>
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</div>
 +
 +
            <!-- Project item -->
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<div class="desktop-four columns tablet-six columns mobile-six columns mix photo project-item">
 +
<a class="popup" href="https://static.igem.org/mediawiki/2015/f/ff/Xuyichen.JPG">
 +
<span class="project-hover">
 +
<span>yichen xu<br>
 +
<b>Photographer</b></span>
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</span>
 +
<img src="https://static.igem.org/mediawiki/2015/f/ff/Xuyichen.JPG" alt="placeholder-thumbnail">
 +
</a>
 +
</div>
 +
           
 +
           
 +
            <!-- Project item -->
 +
<div class="desktop-four columns tablet-six columns mobile-six columns mix photo project-item">
 +
<a class="popup" href="https://static.igem.org/mediawiki/2015/f/f5/Xuzemin.JPG">
 +
<span class="project-hover">
 +
<span>zemin xu<br>
 +
<b>Vice Team Leader and Operation Manager</b></span>
 +
</span>
 +
<img src="https://static.igem.org/mediawiki/2015/f/f5/Xuzemin.JPG" alt="placeholder-thumbnail">
 +
</a>
 +
</div>
 +
           
 +
            <!-- Project item -->
 +
<div class="desktop-four columns tablet-six columns mobile-six columns mix photo project-item">
 +
<a class="popup" href="https://static.igem.org/mediawiki/2015/c/c0/Yinyisu.JPG">
 +
<span class="project-hover">
 +
<span>yisu yin<br>
 +
<b>Public Relationship</b></span>
 +
</span>
 +
<img src="https://static.igem.org/mediawiki/2015/c/c0/Yinyisu.JPG" alt="placeholder-thumbnail">
 +
</a>
 +
</div>
 +
           
 +
                      <!-- Project item -->
 +
<div class="desktop-four columns tablet-six columns mobile-six columns mix movie project-item">
 +
<a class="popup-youtube" href="https://static.igem.org/mediawiki/2015/9/99/Gaoxiaoyuan.JPG">
 +
<span class="project-hover">
 +
<span>xiaoyuan gao<br>
 +
<b>Assistant</b></span>
 +
</span>
 +
<img src="https://static.igem.org/mediawiki/2015/9/99/Gaoxiaoyuan.JPG" alt="placeholder-thumbnail">
 +
</a>
 +
</div>
 +
 +
 +
 +
 +
<!-- Project item -->
 +
<div class="desktop-four columns tablet-six columns mobile-six columns mix website project-item">
 +
<a class="popup" href="https://static.igem.org/mediawiki/2015/a/ad/Zhangwenhao.JPG">
 +
<span class="project-hover">
 +
<span>wenhao zhang<br>
 +
<b>Assistant</b></span>
 +
</span>
 +
<img src="https://static.igem.org/mediawiki/2015/a/ad/Zhangwenhao.JPG" alt="placeholder-thumbnail">
 +
</a>
 +
</div>
 +
 +
<!-- Project item -->
 +
<div class="desktop-four columns tablet-six columns mobile-six columns mix movie project-item">
 +
<a class="popup-vimeo" href="https://static.igem.org/mediawiki/2015/c/cc/Shizhewen.JPG">
 +
<span class="project-hover">
 +
<span>zhewen shi<br>
 +
<b>Assistant</b></span>
 +
</span>
 +
<img src="https://static.igem.org/mediawiki/2015/c/cc/Shizhewen.JPG" alt="placeholder-thumbnail">
 +
</a>
 +
</div>
 +
 +
</div>
 +
<div class="clear"></div>
 +
</section>
 +
 +
<!-- About me -->
 +
<section id="about-me" class="about-me">
 +
 +
<!-- Header with text ticker -->
 +
<div class="header" style="background-image: url('https://static.igem.org/mediawiki/2015/b/bb/Plants.jpg');">
 +
<div class="text-ticker">
 +
<ul>
 +
<li class="fitticker">E.coli&LC3</li>
 +
<li class="fitticker">GFP-LC3</li>
 +
<li class="fitticker">pSB1C3 as Vector</li>
 +
<li class="fitticker">Fluorescent Protein Marker</li>
 +
<li class="fitticker">Autophagosomes</li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
<!-- Title -->
 +
<div class="row">
 +
<div class="ten columns offset-by-one title">
 +
<h1>Project details</h1>
 +
<h2>HOW WE DO IT</h2>
 +
<hr>
 +
</div>
 +
</div>
 +
 +
<!-- About me introduction -->
 +
<div class="row">
 +
<div class="ten columns offset-by-one margin-bottom">
 +
<p>Autophagy gets the potential impact to fight with cancer. However it still get some negative effect. The reaction taken place in the lysosome can consciously can unconsciously help the cancer cell to grow. It may be an efficient assist of deteriorating the denaturation of tumor cell. After all, it still play a paramount significance in the future genetic study.
 +
 +
</p>
 +
 +
</div>
 +
</div>
 +
 +
<!-- About me facts -->
 +
<div class="row text-left">
 +
<div class="one column">
 +
<i class="icon-camera column-icon"></i>
 +
</div>
 +
<div class="five columns">
 +
 +
<p>Competent E.coli cell preparation</p>
 +
                                <p>Transform pIREs2-GFP-LC3 into Competent E.coli cell </p>
 +
</div>
 +
 +
<div class="one column">
 +
<i class="icon-headphones column-icon"></i>
 +
</div>
 +
<div class="five columns">
 +
 +
<p>Restriction enzyme digestion: get GFP-LC3 gene fragment from pIREs2-GFP-LC3 </p>
 +
                                <p>Purification of gene fragment GFP-LC3 </p>
 +
</div>
 +
 +
<div class="one column">
 +
<i class="icon-pencil column-icon"></i>
 +
</div>
 +
<div class="five columns">
 +
 +
<p>Ligation: GFP-LC3+PSB1C3</p>
 +
                                <p>Transform pSB1C3-GFP-LC3 into Competent E.coli cell </p>
 +
</div>
 +
 +
<div class="one column">
 +
<i class="icon-film column-icon"></i>
 +
</div>
 +
<div class="five columns">
 +
 +
<p>Mini-prep of Plasmids PSB1C3-GFP-LC3</p>
 +
                                <p>Electrophoresis and Sequencing</p>
 +
</div>
 +
</div>
 +
</section>
 +
 +
 +
<!-- Resume -->
 +
<section id="resume" class="resume">
 +
 +
<div class="row">
 +
<div class="ten columns offset-by-one title">
 +
<h1>Timeline</h1>
 +
<h2>Our work experiences</h2>
 +
<hr>
 +
</div>
 +
</div>
 +
 +
<!-- Work experience -->
 +
<div class="row margin">
 +
<div class="two columns offset-by-one text-right">
 +
<h2 class="years">23th Dec</h2>
 +
</div>
 +
<div class="eight columns text-left column-border">
 +
 +
<p>iGEM Intro and Potential Member Selection.</p>
 +
</div>
 +
</div>
 +
 +
<!-- Work experience -->
 +
<div class="row margin">
 +
<div class="two columns offset-by-one text-right">
 +
<h2 class="years">27th Jan</h2>
 +
</div>
 +
<div class="eight columns text-left column-border">
 +
 +
<p>Brainstorming and Presentation.</p>
 +
</div>
 +
</div>
 +
 +
<!-- Work experience -->
 +
<div class="row margin">
 +
<div class="two columns offset-by-one text-right">
 +
<h2 class="years">5th Mar</h2>
 +
</div>
 +
<div class="eight columns text-left column-border">
 +
 +
<p>Final Membership Confirmed.</p>
 +
</div>
 +
</div>
 +
 +
        <!-- Work experience -->
 +
<div class="row margin">
 +
<div class="two columns offset-by-one text-right">
 +
<h2 class="years">13th Apr</h2>
 +
</div>
 +
<div class="eight columns text-left column-border">
 +
 +
<p>ZJU Lab Permission Registration.</p>
 +
</div>
 +
</div>
 +
 +
                <!-- Work experience -->
 +
<div class="row margin">
 +
<div class="two columns offset-by-one text-right">
 +
<h2 class="years">17th May</h2>
 +
</div>
 +
<div class="eight columns text-left column-border">
 +
 +
<p>Discussion Conference with ZJU & Peking University.</p>
 +
</div>
 +
</div>
 +
             
 +
                <!-- Work experience -->
 +
<div class="row margin">
 +
<div class="two columns offset-by-one text-right">
 +
<h2 class="years">12th Jun</h2>
 +
</div>
 +
<div class="eight columns text-left column-border">
 +
 +
<p>PCR Operation Skills Training and Preparation for Experiment.</p>
 +
</div>
 +
</div>
 +
               
 +
                <!-- Work experience -->
 +
<div class="row margin">
 +
<div class="two columns offset-by-one text-right">
 +
<h2 class="years">From Jun</h2>
 +
</div>
 +
<div class="eight columns text-left column-border">
 +
 +
<p>Training Every Single Two weeks and Lab Work.</p>
 +
</div>
 +
</div>
 +
 +
                <!-- Work experience -->
 +
<div class="row margin">
 +
<div class="two columns offset-by-one text-right">
 +
<h2 class="years">24th Aug</h2>
 +
</div>
 +
<div class="eight columns text-left column-border">
 +
 +
<p>Final Stage of Our Experiment.</p>
 +
</div>
 +
</div>
 +
 +
<!-- Contact -->
 +
<section class="call-to-action">
 +
<div class="row">
 +
<div class="twelve columns">
 +
<h3>Sponsors</h3>
 +
<p>
 +
                                        <img src="https://static.igem.org/mediawiki/2015/d/d9/Sponsor.png" alt="sponsors">
 +
</p>
 +
</div>
 +
</div>
 +
</section>
 +
       
 +
        <section class="call-to-action">
 +
<div class="row">
 +
<div class="twelve columns">
 +
<h3>CONTACT US</h3>
 +
                                <p>No.436, No.6 Road, Xiasha</p>
 +
<p>Hangzhou,Zhejiang, China</p>
 +
                                <p>Phone:+8613957137482</p>
 +
                                <p>Mail:ricardo937@outlook.com</p>
 +
</div>
 +
</section>
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 +
 +
<div id="loader">
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Latest revision as of 13:52, 14 September 2015

Focus - Personal Edition

  • iGEM HANGZHOU

    More accurate and distinct observation? Special fluorescent protein marker.

    LEARN MORE

  • Cancer? Autophagy?

    Find a link between them.

    Experience more

  • Human Practice

    You will see our excellent work.

  • The Truth Is Going to be revealed below.

    Experience more

Project

This is what We do

  • background

    Our IGEM research project involves study of the autophagy phenomenon by using the GFP to reveal it more clearly. The Autophagy is a biological process which acts like a recycle factory. Like you put a pop can into the trash bin and then reproduce by the factory, the procedure of autophagy just catch the aging, denaturing, damaged organelles and send them into the lysosome which degrades the organelles.

  • Health

    Autophagy is developed as a way to address the disease and maintain the health of human body. With the degradation of the denaturing cellular, our cells can have the ability to defend themselves without other medical treatment.

  • Anti-aging

    Autophagy is also capable to keep us young and strong. It is better than the cosmetic we used which may cause other problem.

  • Fight with cancer.

    Autophagy gets the potential impact to fight with cancer. However it still get some negative effect. The reaction taken place in the lysosome can consciously can unconsciously help the cancer cell to grow. It may be an efficient assist of deteriorating the denaturation of tumor cell. After all, it still play a paramount significance in the future genetic study.

Human Practice

How We do it

Environmental Impact

Since our subject is a fundamental work in this field, all the processes were finished in the laboratory. It did not bring any negative effects to our environment.

Safety

The experiment itself is totally safe. Although we met some trouble during the operation of experiment, fortunately, all the troubles did not cause any hurt to teammates or others.

Education

In order to make the public have deeper comprehending of genetic modification, we did a lecture and a questionnaire of people about GM food. The reason why we used the GM food as the topic but not the subject of our experiment was that our subject is too professional for normal people to understand.

Evaluation

We summarized and processed our statistics. It is not surprised that the students accept GM food in a much larger extent than that in general public.

Portfolio

  • E.coli&LC3
  • GFP-LC3
  • pSB1C3 as Vector
  • Fluorescent Protein Marker
  • Autophagosomes

Project details

HOW WE DO IT

Autophagy gets the potential impact to fight with cancer. However it still get some negative effect. The reaction taken place in the lysosome can consciously can unconsciously help the cancer cell to grow. It may be an efficient assist of deteriorating the denaturation of tumor cell. After all, it still play a paramount significance in the future genetic study.

Competent E.coli cell preparation

Transform pIREs2-GFP-LC3 into Competent E.coli cell

Restriction enzyme digestion: get GFP-LC3 gene fragment from pIREs2-GFP-LC3

Purification of gene fragment GFP-LC3

Ligation: GFP-LC3+PSB1C3

Transform pSB1C3-GFP-LC3 into Competent E.coli cell

Mini-prep of Plasmids PSB1C3-GFP-LC3

Electrophoresis and Sequencing

Timeline

Our work experiences

23th Dec

iGEM Intro and Potential Member Selection.

27th Jan

Brainstorming and Presentation.

5th Mar

Final Membership Confirmed.

13th Apr

ZJU Lab Permission Registration.

17th May

Discussion Conference with ZJU & Peking University.

12th Jun

PCR Operation Skills Training and Preparation for Experiment.

From Jun

Training Every Single Two weeks and Lab Work.

24th Aug

Final Stage of Our Experiment.

Sponsors

sponsors

CONTACT US

No.436, No.6 Road, Xiasha

Hangzhou,Zhejiang, China

Phone:+8613957137482

Mail:ricardo937@outlook.com

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