Difference between revisions of "Team:KU Leuven/Research/Methods"
Line 199: | Line 199: | ||
</div> | </div> | ||
<div id="togglefive"> | <div id="togglefive"> | ||
− | <p><b>Materials:</b | + | <p><b>Materials:</b> |
<dl> | <dl> | ||
<dd>DNA</dd> | <dd>DNA</dd> | ||
Line 205: | Line 205: | ||
<dd>SOC</dd> | <dd>SOC</dd> | ||
<dd>ice-cold cuvettes</dd> | <dd>ice-cold cuvettes</dd> | ||
− | </dl> | + | </dl><br> |
+ | <b>Protocol<b> | ||
+ | Add 1 µl DNA to 50 µl electrocompetent cells in an ice-cold cuvette (1 mm)</br> | ||
+ | Electroporate (Eppendorf, 1700 V, 4 msec)</br> | ||
+ | Add 950 µl of SOC solution</br> | ||
+ | Incubate for one hour at 37 °C</br> | ||
+ | Plate this out on pre-warmed plates (37 °C)</br> | ||
+ | J23101, J23106 and J23117 were plated out on chloramphenicol and I13504 was | ||
+ | plated out on ampicillin</br></p> | ||
</div> | </div> |
Revision as of 14:51, 14 September 2015
Methods
On this page you can find all of the methods and protocols used in the lab to obtain our results. For some techniques, we included some basic theory, since it is a prerequisite to get acquainted with the theory behind these techniques before using them. To learn more about them, click the titles below!