Difference between revisions of "Team:KU Leuven/Research/Methods"
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<p><b>Protocol</b></p> | <p><b>Protocol</b></p> | ||
<dl> | <dl> | ||
− | <dd>1. Prepare selective media (LB with 0.25% agar (2,5 g/L) in Petri dishes (85 mm dia | + | <dd>1. Prepare selective media (LB with 0.25% agar (2,5 g/L) in Petri dishes (85 mm dia).</dd> |
− | <dd>2. Apply 1.5 µL of the diluted cell suspensions from mid-log-phase cultures (~2×105 cells/µL (OD=0.5)) to the center of the plates, and let dry in air for 15 min </dd> | + | <dd>2. Apply 1.5 µL of the diluted cell suspensions from mid-log-phase cultures (~2×105 cells/µL (OD=0.5)) to the center of the plates, and let dry in air for 15 min. </dd> |
<dd>3. Incubate at 37 °C for 10 h. </dd> | <dd>3. Incubate at 37 °C for 10 h. </dd> | ||
</dl> | </dl> |
Revision as of 15:34, 16 September 2015
Methods
On this page you can find all of the methods and protocols used in the lab to obtain our results. For some techniques, we included some basic theory, since it is a prerequisite to get acquainted with the theory behind these techniques before using them. To learn more about them, click the titles below!
Contact
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be