Difference between revisions of "Safety/Final Safety Form"

(updated the links for uploading and viewing your spreadsheet. They will not work with CSV... but I shall assume that anyone working with CSV is at least a little tech-savvy.)
(Switched back to 2015 copy of form.cgi, Randy reports he fixed it)
 
(22 intermediate revisions by the same user not shown)
Line 1: Line 1:
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{{2015Top}}
+
{{2015Top}} {{JS/QueryStringUtilities}} {{HTML/JSDisabled}}
−
 
+
 
<html>
 
<html>
  
 
<link rel="stylesheet" type="text/css" href="https://igem.org/Template:CSS/Forms?action=raw&ctype=text/css" />
 
<link rel="stylesheet" type="text/css" href="https://igem.org/Template:CSS/Forms?action=raw&ctype=text/css" />
−
<!-- include KForms here -->
+
<style type="text/css">
−
<!-- also remember to config form_info -->
+
#safety_peas_container {
 +
width: 250px;
 +
float: right;
 +
position: relative;
 +
top: -50px;
 +
}
 +
#safety_peas_container, #safety_peas_container td {
 +
border: none;
 +
}
 +
#safety_peas_container img {
 +
width: 125px;
 +
}
 +
#safety_peas_container p {
 +
font-size: x-small;
 +
}
 +
</style>
 +
 
 +
<script type="text/javascript">
 +
var form_info = {};
 +
form_info.name="2015_final_safety_form";
 +
form_info.display_title='2015 Final Safety Form';
 +
form_info.default_mode = "edit";
 +
form_info.owner_type = "team";
 +
form_info.permissions = {view: ["public"],
 +
edit: ["group_members"],
 +
submit: ["Instructor", "Primary Contact"],
 +
admin: ["super_users"]};
 +
form_info.validate_unspecified_fields = "required";
 +
form_info.ajax_URL = "https://2015.igem.org/cgi/forms/form.cgi";
 +
</script>
 +
<script src="https://igem.org/Template:JS/KForms_2.3?action=raw&ctype=text/javascript"></script>
  
 
<!-- this is where the submenu is placed -->
 
<!-- this is where the submenu is placed -->
Line 16: Line 45:
  
 
<!-- alert message, the text can be edited -->  
 
<!-- alert message, the text can be edited -->  
−
<div class="alertMessage"> <p>Safety form software is still being written. The form will be open for answers at the beginning of May. We apologize for the delay.</p></div>
+
<!--<div class="alertMessage"> <p> Safety form software is still being written. The form will be open for answers at the beginning of May. We apologize for the delay.</p></div>-->
  
−
<div id="formbody">
+
<table id="safety_peas_container">
 +
<tr>
 +
<td colspan="2">
 +
<img src="https://static.igem.org/mediawiki/igem.org/c/cd/Students_fill_safety_form.png" class="safety_peas">
 +
<p>Students complete Final Safety Form (Deadline: August 28)</p>
 +
</td>
 +
</tr>
 +
<tr>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/igem.org/3/30/Instructor_approves_safety_form.png" class="safety_peas">
 +
<p>Instructor reads and approves Final Safety Form</p>
 +
</td>
 +
<td>
 +
<img src="https://static.igem.org/mediawiki/igem.org/e/e1/Submit_safety_form.png" class="safety_peas">
 +
<p>Instructor submits form (from his or her user account)</p>
 +
</td>
 +
</tr>
 +
</table>
  
−
<p class="question">1. What is your chassis organism?</p>
+
<h3>Due August 28, 2015</h3>
−
<p>Check all species you are genetically modifying in your project.</p>
+
−
 
+
−
<ul>
+
−
<li><label><input disabled type="checkbox" name="Chassis organism" data-form-field="ecoli"> E. coli</label></li>
+
−
<li><label><input disabled type="checkbox" name="Chassis organism" data-form-field="yeast"> Yeast (Saccharomyces)</label></li>
+
−
<li><label><input disabled type="checkbox" name="Chassis organism" data-form-field="lactobacillus"> Lactobacillus</label></li>
+
−
<li><label><input disabled type="checkbox" name="Chassis organism" data-form-field="bsubtilis"> B. subtilis</label></li>
+
−
<li><label><input disabled type="checkbox" name="Chassis organism" data-form-field="chassis_other"> Others: </label></li>
+
−
<li><label><input disabled type="checkbox" name="Chassis organism" data-form-field="chassis_none"> No chassis organism</label></li>
+
−
</ul>
+
−
 
+
−
<p class="question">Comments:</p>
+
−
 
+
−
<p class="question">2. Do you plan to experiment with any other organisms, besides your chassis?</p>
+
−
<p>What organisms, and what experiments will you do? Please explain briefly. Please include the names of species / cell lines / strains.</p>
+
  
−
<textarea disabled rows="4" name="Other organisms" data-form-field="other_organisms"></textarea>
+
<p>This form is for you to tell us all about your project, the organisms/parts you are using, the potential risks of your project, and what you are doing to reduce those risks.</p>
  
−
<p>Example answers:</p>
 
 
<ul>
 
<ul>
−
<li>"Our bacteria is meant to live on plant leaves, so we will test them on tobacco (Nicotiana benthamiana) in a lab greenhouse."</li>
+
<li><strong>We encourage STUDENTS, instead of instructors, to complete this form. However, you will need an Instructor (or Primary Contact) to sign and submit this form.</strong></li>
−
<li>"We want to use a protein from ants, but its sequence is unknown. So we will capture ants (Camponotus spp.) to extract DNA and RNA to find the sequence of the protein we want."</li>
+
<li>While you type, this form will remember your answers. When you are finished, press the "Submit" button at the bottom to send your form to the iGEM Safety Committee.</li>
−
<li>"Our bacteria need to interact with human cells for a medical application. We will test them in human cell culture using the HEK293 cell line."</li>
+
<li>Submit this form by Friday, August 28, 2015.</li>
 +
<li>If you will not be able to complete this form before the deadline, email us (safety AT igem DOT org) before August 28 and tell us about your situation.</li>
 
</ul>
 
</ul>
  
−
<p class="question">3. How will your project work?</p>
+
</html>{{HTML/Temp/FormTitleAndMode}}<html>
−
<p>Describe the goal of your project: what is your engineered organism supposed to do? Please include specific technical details and names of important parts. (Even though your project might change, please describe the main project idea you are working on right now. See the example answers for help.)</p>
+
  
−
<textarea disabled rows="4" name="Other organisms" data-form-field="other_organisms"></textarea>
+
<div id="formbody" class="bordered">
  
−
<p>Good example answers:</p>
+
<fieldset>
−
<ul>
+
<legend class="qnum">Team member who should be contacted about this form:</legend>
−
<li>"Our bacteria will live inside a human body. They will detect tumor cells that express biomarkers for liver cancer. They will use invasin to enter the tumor cells, and then secrete apoptin to kill the tumor cells."</li>
+
<p>Name <input type="text" name="Team member Name" data-form-field="corr_team_member_name"></p>
−
<li>"Our algae will receive the exhaust from a factory, which is high in CO2. We will increase their expression of Photosystem II proteins to make them absorb more CO2, reducing the factory's emissions."</li>
+
<p>Email <input type="text" name="Team member Email" data-validate-as="email" class="validate-on-change" data-form-field="corr_team_member_email"></span></p>
−
</ul>
+
</fieldset>
−
 
+
−
<p>Bad example answers (not enough detail):</p>
+
−
<ul>
+
−
<li>"We are engineering E. coli to cure liver cancer."</li>
+
−
<li>"Climate change is a very important problem. Our algae will reduce CO2 emissions and fight climate change."</li>
+
−
</ul>
+
−
 
+
−
<p class="question">4. How would your project be used in the real world?</p>
+
−
<p>Imagine that your project were fully developed into a real product that real people could use. How would people use it? Check all appropriate boxes.</p>
+
−
<p>(Note: iGEM teams should not release modified organisms into the natural environment.)</p>
+
−
 
+
−
<ul>
+
−
<li><label><input disabled type="checkbox" name="Real world application" data-form-field=""> Our project is foundational / we do not have a specific real-world application in mind</label><br />
+
−
(Examples: library of standardized promoters, system for communication between cells)</li>
+
−
<li><label><input disabled type="checkbox" name="Real world application" data-form-field=""> Only in the lab</label><br />
+
−
(Examples: reporter strain for measuring the strength of promoters)</li>
+
−
<li><label><input disabled type="checkbox" name="Real world application" data-form-field=""> In a factory</label><br />
+
−
(Examples: cells that make a flavor chemical for food, cells that make biofuel)</li>
+
−
<li><label><input disabled type="checkbox" name="Real world application" data-form-field=""> In a consumer product that ordinary people buy</label><br />
+
−
(Examples: cells that clean your clothes, bread made with engineered yeast)</li>
+
−
<li><label><input disabled type="checkbox" name="Real world application" data-form-field=""> In agriculture / on a farm</label><br />
+
−
(Examples: cells that guard against pests, engineered rice plants, cells that promote growth of crop plants)</li>
+
−
<li><label><input disabled type="checkbox" name="Real world application" data-form-field=""> In a small enclosed device</label><br />
+
−
(Examples: a bio-sensing strip with cells that detect arsenic)</li>
+
−
<li><label><input disabled type="checkbox" name="Real world application" data-form-field=""> In the natural environment</label><br />
+
−
(Examples: cells that remove pollution from lakes, engineered forest trees that can resist drought)</li>
+
−
<li><label><input disabled type="checkbox" name="Real world application" data-form-field=""> To be used in the human body, or in food</label><br />
+
−
(Examples: anti-cancer bacteria, bread made with engineered yeast, engineered rice plants)</li>
+
−
<li><label><input disabled type="checkbox" name="Real world application" data-form-field=""> Other</label><br />
+
−
(Examples: bacteria that live on Mars)</li>
+
−
</ul>
+
  
 
<fieldset>
 
<fieldset>
−
<legend class="qnum">Your New Parts</legend>
+
<legend class="qnum">1. Your New Parts</legend>
  
 
<br />
 
<br />
Line 123: Line 122:
 
<br />
 
<br />
 
</fieldset>
 
</fieldset>
 +
 +
<br />
 +
<p class="question"><span class="qnum">2.</span> What is your chassis organism?</p>
 +
<p>Check all species you are genetically modifying in your project.</p>
 +
 +
<ul>
 +
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_ecoli"> E. coli (lab strains that are not harmful to humans)</label></li>
 +
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_yeast"> Yeast (Saccharomyces)</label></li>
 +
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_lactobacillus"> Lactobacillus</label></li>
 +
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_bsubtilis"> B. subtilis</label></li>
 +
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_other"> Others (give species names): </label> <textarea rows="2" name="Other chassis (give species names)" data-form-field="chassis_other_describe" data-validation="[data-form-field=chassis_other]"></textarea></li>
 +
<li><label><input type="checkbox" name="Chassis organism" data-form-field="chassis_none"> No chassis organism (please comment):</label> <textarea rows="2" name="No chassis (comment)" data-form-field="chassis_none_describe" data-validation="[data-form-field=chassis_none]"></textarea></li>
 +
</ul>
 +
 +
<p class="question">Comments:</p>
 +
<textarea rows="2" name="Chassis organism -- comments" data-form-field="chassis_comments"></textarea>
 +
 +
<br />
 +
<p class="question"><span class="qnum">3.</span> Do you plan to experiment with any other organisms, besides your chassis?</p>
 +
<p>What organisms, and what experiments will you do? Please explain briefly. Please include the names of species / cell lines / strains.</p>
 +
 +
<textarea rows="4" name="Other organisms" data-form-field="other_organisms"></textarea>
 +
 +
<div class="highlightBoxB">
 +
<a href="#" class="click_expand">Example answers...</a>
 +
<div>
 +
<ul>
 +
<li>"Our bacteria is meant to live on plant leaves, so we will test them on tobacco (Nicotiana benthamiana) in a lab greenhouse."</li>
 +
<li>"We want to use a protein from ants, but its sequence is unknown. So we will capture ants (Camponotus spp.) to extract DNA and RNA to find the sequence of the protein we want."</li>
 +
<li>"Our bacteria need to interact with human cells for a medical application. We will test them in human cell culture using the HEK293 cell line."</li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
<br />
 +
<p class="question"><span class="qnum">4.</span> How will your project work?</p>
 +
<p>Describe the goal of your project: what is your engineered organism supposed to do? Please include specific technical details and names of important parts. (Even though your project might change, please describe the main project idea you are working on right now. See the example answers for help.)</p>
 +
 +
<textarea rows="6" name="How your project works" data-form-field="how_it_works"></textarea>
 +
 +
<div class="highlightBoxB">
 +
<a href="#" class="click_expand">Example answers...</a>
 +
<div>
 +
<p>Good example answers:</p>
 +
<ul>
 +
<li>"Our bacteria will live inside a human body. They will detect tumor cells that express biomarkers for liver cancer. They will use invasin to enter the tumor cells, and then secrete apoptin to kill the tumor cells."</li>
 +
<li>"Our algae will receive the exhaust from a factory, which is high in CO2. We will increase their expression of Photosystem II proteins to make them absorb more CO2, reducing the factory's emissions."</li>
 +
</ul>
 +
 +
<p>Bad example answers (not enough detail):</p>
 +
<ul>
 +
<li>"We are engineering E. coli to cure liver cancer."</li>
 +
<li>"Climate change is a very important problem. Our algae will reduce CO2 emissions and fight climate change."</li>
 +
</ul>
 +
</div>
 +
</div>
 +
 +
<br />
 +
<p class="question"><span class="qnum">5.</span> What risks does your project pose at the laboratory stage? What actions are you taking to reduce those risks?</p>
 +
<p>If you are working in a biology lab, you cannot answer "no risks". Even the simplest experiment, with the safest bacteria, poses some small risk. The actions you take to reduce that risk would include safety level 1 procedures, wearing rubber gloves, sterilizing waste, etc.</p>
 +
 +
<textarea rows="6" name="Risks of your project in the lab" data-form-field="project_lab_risks"></textarea>
 +
 +
<br />
 +
<p class="question"><span class="qnum">6.</span> How would your project be used in the real world?</p>
 +
<p>Imagine that your project were fully developed into a real product that real people could use. How would people use it? Check all appropriate boxes.</p>
 +
<p>(Note: iGEM teams should not release modified organisms into the natural environment.)</p>
 +
 +
<ul>
 +
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_foundational"> Our project is foundational / we do not have a specific real-world application in mind</label><br />
 +
(Examples: library of standardized promoters, system for communication between cells)</li>
 +
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_lab"> Only in the lab</label><br />
 +
(Examples: reporter strain for measuring the strength of promoters)</li>
 +
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_factory"> In a factory</label><br />
 +
(Examples: cells that make a flavor chemical for food, cells that make biofuel)</li>
 +
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_consumer"> In a consumer product that ordinary people buy</label><br />
 +
(Examples: cells that clean your clothes, bread made with engineered yeast)</li>
 +
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_agriculture"> In agriculture / on a farm</label><br />
 +
(Examples: cells that guard against pests, engineered rice plants, cells that promote growth of crop plants)</li>
 +
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_device"> In a small enclosed device</label><br />
 +
(Examples: a bio-sensing strip with cells that detect arsenic)</li>
 +
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_environment"> In the natural environment</label><br />
 +
(Examples: cells that remove pollution from lakes, engineered forest trees that can resist drought)</li>
 +
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_humanbody"> To be used in the human body, or in food</label><br />
 +
(Examples: anti-cancer bacteria, bread made with engineered yeast, engineered rice plants)</li>
 +
<li><label><input type="checkbox" name="Real world application" data-form-field="rwa_other"> Other</label><br />
 +
(Examples: bacteria that live on Mars)</li>
 +
</ul>
 +
 +
<p class="question">Comments:</p>
 +
<textarea rows="2" name="Real world application -- comments" data-form-field="rwa_comments"></textarea>
 +
 +
<br />
 +
<p class="question"><span class="qnum">7.</span> What risks might your project pose, if it were fully developed into a real product that real people could use? What future work might you do to reduce those risks?</p>
 +
 +
<textarea rows="5" name="Risks of your project in the future" data-form-field="project_future_risk"></textarea>
 +
 +
<br />
 +
<p class="question"><span class="qnum">8.</span> Any further comments about your project:</p>
 +
 +
<textarea rows="3" data-validation="optional" name="Further comments" data-form-field="further_comments"></textarea>
 +
 +
<br />
 +
<p class="question"><span class="qnum">9.</span> Comments about this form: Is it easy or difficult to use? Are the questions confusing?</p>
 +
 +
<textarea rows="2" data-validation="optional" name="Comments about this form" data-form-field="comments_about_this_form"></textarea>
  
 
<fieldset>
 
<fieldset>
Line 128: Line 233:
 
<p>Only a team Instructor (or Primary Contact) may submit the Safety Form.</p>
 
<p>Only a team Instructor (or Primary Contact) may submit the Safety Form.</p>
 
<p>Instructors, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen.</p>
 
<p>Instructors, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen.</p>
−
<DIV id="submit_button_container" style="position: relative;">
+
 
 +
<br />
 +
<DIV class="submit_buttons_box">
 
<input class="confirmation" autocomplete="off" type="checkbox" name="I Agree" id="i_agree">  
 
<input class="confirmation" autocomplete="off" type="checkbox" name="I Agree" id="i_agree">  
−
<label for="i_agree"><strong>I Agree</strong></label>  
+
<label for="i_agree" class="question">I Agree</label>  
 
&nbsp;&nbsp;&nbsp;&nbsp;  
 
&nbsp;&nbsp;&nbsp;&nbsp;  
 
<input type="submit" data-form-field="submit" data-confirmation="i_agree" value="Submit Form">
 
<input type="submit" data-form-field="submit" data-confirmation="i_agree" value="Submit Form">
 +
<p class="question">Need to make changes? <input type="submit" data-form-field="return_form" value="Unsubmit Form"></p>
 
</DIV>
 
</DIV>
 
<br />
 
<br />
−
<p><input type="submit" data-form-field="return_form" value="Unsubmit Form"></p>
 
−
</p>
 
 
</fieldset>
 
</fieldset>
 +
 +
</html>{{HTML/Temp/FormsAdmin}}<html>
  
 
</div>
 
</div>
Line 149: Line 257:
  
 
<script type="text/javascript">
 
<script type="text/javascript">
 +
function custom_get_owner_info_response(data, textStatus, jqxhr) {
 +
console.log("custom get owner info response called");
 +
console.log(jqxhr.responseJSON.track);
 +
spreadsheet_access();
 +
}
 +
var lastreq;
 +
function custom_one_input_changed_response(input_element, command, effective_date, data, textStatus, jqxhr) {
 +
console.log("OIC custom callback");
 +
console.log(jqxhr.responseJSON.return_error);
 +
lastreq = jqxhr;
 +
}
 +
 
//**Call this function in one of the custom-when-team-info-gotten callbacks
 
//**Call this function in one of the custom-when-team-info-gotten callbacks
 
function spreadsheet_access() {
 
function spreadsheet_access() {
−
if(typeof team_name == "string") {
+
if(typeof owner_info !== "undefined") {
 +
//console.log("Setting spreadsheet links for team " + owner_info.team_name);
 
var url_start = "https://2015.igem.org/wiki/index.php?title=Special:Upload\u0026wpDestFile="
 
var url_start = "https://2015.igem.org/wiki/index.php?title=Special:Upload\u0026wpDestFile="
 
var url_end = "_Safety2015_Spreadsheet.xls";
 
var url_end = "_Safety2015_Spreadsheet.xls";
−
var whole_url = url_start + team_name + url_end;
+
var whole_url = url_start + owner_info.team_name + url_end;
 
jQuery("#spreadsheet_upload").attr("href", whole_url);
 
jQuery("#spreadsheet_upload").attr("href", whole_url);
 
 
−
jQuery("#spreadsheet_view").attr("href", "https://2015.igem.org/File:" + team_name + "_Safety2015_Spreadsheet.xls");
+
jQuery("#spreadsheet_view").attr("href", "https://2015.igem.org/File:" + owner_info.team_name + "_Safety2015_Spreadsheet.xls");
 +
}
 +
else {
 +
//console.log("Blanking spreadsheet links");
 +
jQuery("#spreadsheet_upload").attr("href", "");
 +
jQuery("#spreadsheet_view").attr("href", "");
 
}
 
}
 
}
 
}

Latest revision as of 16:24, 16 September 2015


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Students complete Final Safety Form (Deadline: August 28)

Instructor reads and approves Final Safety Form

Instructor submits form (from his or her user account)

Due August 28, 2015

This form is for you to tell us all about your project, the organisms/parts you are using, the potential risks of your project, and what you are doing to reduce those risks.

  • We encourage STUDENTS, instead of instructors, to complete this form. However, you will need an Instructor (or Primary Contact) to sign and submit this form.
  • While you type, this form will remember your answers. When you are finished, press the "Submit" button at the bottom to send your form to the iGEM Safety Committee.
  • Submit this form by Friday, August 28, 2015.
  • If you will not be able to complete this form before the deadline, email us (safety AT igem DOT org) before August 28 and tell us about your situation.

This form has been submitted.

You can unsubmit the form if you wish to make further edits.

Click here to dismiss this message

-- Please choose a team

Team member who should be contacted about this form:

Name

Email

1. Your New Parts

Please visit this page to download a blank copy of the spreadsheet for this question. (If you need a CSV version instead of XLS, visit this page.)

Complete the spreadsheet. Include all whole organisms that you will handle in the lab, whether you are using them as a chassis or for some other reason. Include all new or highly modified protein coding parts that you are using. If you submitted a Check-In for an organism or part, you should still include it in this spreadsheet.

You may omit non-protein-coding parts, and you may omit parts that were already in the Registry if you are using them without significant modifications.


Click here to show/hide instructions for completing the spreadsheet

Remember to change the filename of your spreadsheet! Put your team's name in place of "TeamName".

  1. Species name (including strain): For an organism, give the scientific name of the species. Include a strain name or number (such as "K-12" for E. coli K-12) if there is one. For a part, give the name and strain of the organism that the part originally came from.
  2. Risk Group: Give the Risk Group of the organism in column A. You may use a categorization according to your home country, according to the USA, or according to the WHO. If the organism falls into an 'in-between' or special category such as 2+ or 2-Agricultural, explain this category in the Notes column. If you cannot find any Risk Group categorization for this organism, write "N/A" and explain in the Notes column. (Multicellular organisms generally do not have a Risk Group.)
  3. Risk Group Source: Cite the source from which you obtained the Risk Group information. See Risk Group Guide for recommended sources. If you got the information from the Canadian PSDS, from the NIH Guidelines, or from the DSMZ catalogue, you may simply write "PSDS", "NIH", or "DSMZ". Otherwise, please give a web link or a full citation for your source.
  4. Disease risk to humans?: Does this organism cause any disease in humans? If yes, what disease does it cause?
  5. Part number/name: For a part: If it has a Registry part number (like BBa_XXXXX), write that number. If it has no Registry part number, give a short name for the part. (For example: "Actin", "Alcohol Dehydrogenase".) For a whole organism, leave this column blank.
  6. Natural function of part: For a part: Briefly describe what the part does in its parent organism. (If it is an enzyme, what reaction does it catalyze? If it is a receptor, what molecules does it bind to? Etc.) For a whole organism, leave this column blank.
  7. How did you acquire it?: Describe how you acquired the organism/part. If you have not acquired it yet, describe how you plan to acquire it. (For example: did you receive the part DNA from another lab? Did you order the part DNA from a synthesis company? Did you use PCR to isolate the part from genomic DNA of its parent organism? Did you order the cell line from a company?)
  8. How will you use it?: Describe how you are using the organism/part in the lab. (For example: "This organism is our chassis." "This part senses when the cells are exposed to glucose." "This organism is the source for a part that we are isolating by PCR." "This part produces the toxin which our bio-sensor is designed to detect.")
  9. Notes: Use this column to give any additional information that is necessary.

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2. What is your chassis organism?

Check all species you are genetically modifying in your project.

Comments:


3. Do you plan to experiment with any other organisms, besides your chassis?

What organisms, and what experiments will you do? Please explain briefly. Please include the names of species / cell lines / strains.

Example answers...
  • "Our bacteria is meant to live on plant leaves, so we will test them on tobacco (Nicotiana benthamiana) in a lab greenhouse."
  • "We want to use a protein from ants, but its sequence is unknown. So we will capture ants (Camponotus spp.) to extract DNA and RNA to find the sequence of the protein we want."
  • "Our bacteria need to interact with human cells for a medical application. We will test them in human cell culture using the HEK293 cell line."

4. How will your project work?

Describe the goal of your project: what is your engineered organism supposed to do? Please include specific technical details and names of important parts. (Even though your project might change, please describe the main project idea you are working on right now. See the example answers for help.)

Example answers...

Good example answers:

  • "Our bacteria will live inside a human body. They will detect tumor cells that express biomarkers for liver cancer. They will use invasin to enter the tumor cells, and then secrete apoptin to kill the tumor cells."
  • "Our algae will receive the exhaust from a factory, which is high in CO2. We will increase their expression of Photosystem II proteins to make them absorb more CO2, reducing the factory's emissions."

Bad example answers (not enough detail):

  • "We are engineering E. coli to cure liver cancer."
  • "Climate change is a very important problem. Our algae will reduce CO2 emissions and fight climate change."

5. What risks does your project pose at the laboratory stage? What actions are you taking to reduce those risks?

If you are working in a biology lab, you cannot answer "no risks". Even the simplest experiment, with the safest bacteria, poses some small risk. The actions you take to reduce that risk would include safety level 1 procedures, wearing rubber gloves, sterilizing waste, etc.


6. How would your project be used in the real world?

Imagine that your project were fully developed into a real product that real people could use. How would people use it? Check all appropriate boxes.

(Note: iGEM teams should not release modified organisms into the natural environment.)


  • (Examples: library of standardized promoters, system for communication between cells)

  • (Examples: reporter strain for measuring the strength of promoters)

  • (Examples: cells that make a flavor chemical for food, cells that make biofuel)

  • (Examples: cells that clean your clothes, bread made with engineered yeast)

  • (Examples: cells that guard against pests, engineered rice plants, cells that promote growth of crop plants)

  • (Examples: a bio-sensing strip with cells that detect arsenic)

  • (Examples: cells that remove pollution from lakes, engineered forest trees that can resist drought)

  • (Examples: anti-cancer bacteria, bread made with engineered yeast, engineered rice plants)

  • (Examples: bacteria that live on Mars)

Comments:


7. What risks might your project pose, if it were fully developed into a real product that real people could use? What future work might you do to reduce those risks?


8. Any further comments about your project:


9. Comments about this form: Is it easy or difficult to use? Are the questions confusing?

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Only a team Instructor (or Primary Contact) may submit the Safety Form.

Instructors, please read the form you are submitting, and confirm that all its information is correct. By checking the "I Agree" box and clicking the "Submit" button, you are agreeing that the Safety Form accurately describes the activities of your team. We are using the "I Agree" box in lieu of a signature with paper and pen.


    

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