Difference between revisions of "Team:SCUT/Attributions"

Line 365: Line 365:
 
<div class="clear"></div>
 
<div class="clear"></div>
 
 
<p>
+
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
            General Support</p>
+
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
MntA transport
+
color:#333333'>Ruiming Xiao, Xiaojing Li and Jionghui Li managed </span><span
Yu Zhong and Xiaojun Cai were responsible for the combination of constitutive promoter added in front of the MntA protein with the double terminator bihind. After that, they just tested the parameters of the relationship of the concentration of cadmium out and in the E.coli. They spent all their summer holiday finishing it.
+
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif'>CsgA
Cadmium adsorption
+
transport <span style='color:#333333'>part. Ruiming Xiao and Xiaojing Li was in
Ruiming Xiao, Xiaojing Li and Jionghui Li managed CsgA transport part. Ruiming Xiao and Xiaojing Li was in charge of the construction of PcadA +CsgA-EC(n)+TetR+RFP+ter., Jionghui Lialso made efforts to assist both of them. Ruiming Xiao made brilliant contributions and devoted himself to testing the MerR/CadA operon using red fluorescent protein and he also helped Li to measure the AU of the RFP. As for Xiaojing Li, she was diligent and she made great efforts to construct different number of EC protein in order to find the best one. Also, because of deleting the wild CsgA will help efficiently express EC, So Minghui Deng and Yulong Zhang were responsible for the CsgA deletion.
+
charge of </span>the<span style='color:#333333'> construction of P<sub>cadA </sub>+CsgA-EC<sub>(n)</sub>+TetR+RFP+ter.,
Sensor system
+
Jionghui Lialso made efforts to assist both of them. Ruiming Xiao made
Xiaomei Lin and Xunxun Luo buried themselves in this part. They just made the device such as Ptet+cjblue+ter. And also, Xiaomei Li had finished the measurement work with the help of Xiaojing Li. In addition, combining with the device of CsgA transport system, Li and Lin successfully tested the fluorescent protein and checked if the system worked.
+
brilliant contributions and devoted himself to testing the MerR/CadA operon
Design work
+
using red fluorescent protein and he also helped Li to measure the AU of the
Yihong Gu, Shuoyu Chen, Jiahui Zhang and Yunan Lin had undertaken all the tasks of designing of the whole team. They did a lot work including logo, ppt templates, wiki and uniforms. Taking step by step, as a result of clarified division and efficient cooperation, they sophisticatedly design uniforms and posters for the IGEM. And Manfredi helped modify the wiki content.
+
RFP. As for Xiaojing Li, she was diligent and she made great efforts to
Modeling Part
+
construct different number of EC protein in order to find the best one. Also,
Junying Su is responsible for final determination of mathematical model for our project. He devotes to modeling the function of our engineering E.coli accurately. In addition, he writes Parameters Measuring and Data Processing Schemes for four parts, including MntA Transport Model, PcadA Strength Model, csgA Polymerization Model and csgA-ECn Adsorption Model. He also assists the members of experiment group to finish measuring work.
+
because of deleting the wild CsgA will help efficiently express EC, So Minghui
Xiaobin Huang is responsible for studying the mechanism of MntA transport. In order to find out a suitable mathematical model, he has also studied the mechanism of sodium potassium pump and calcium pump, which are also the members of the P-type family of adenosine
+
Deng and Yulong Zhang were responsible for the CsgA deletion.</span><o:p></o:p></span></p>
triphosphatases (ATPases). At the same time, he devotes to establishing the model of Ptet Strength. He has found out two available models in the previous iGEM work of Team EPF-Lausanne 2008 and Team UC Davis 2013 respectively. [1][2] Additionally, he works in data processing.
+
Chongyin Han is responsible for studying the mechanism of csgA polymerization. In order to find out a suitable mathematical model, he has studied an available model in the work of Team INSA-Lyon 2014. [3] Whether this model is useful for us, we need further verification.
+
Nana Pang is responsible for studying the mechanism of PcadA and merR mutant, and devotes to establishing the model of PcadA Strength. In order to figure out the accurate concentration of cadmium ion at which PcadA takes obvious effect, she analyzes the combination process of cadmium ion and merR.
+
Jing Liao is responsible for studying the mechanism of csgA-ECn adsorption. She devotes to working out the value of disassociation constant Kd with the available data. In addition, she tries to figure out the optimal value of csgA-ECn length n to improve the ability of cadmium ion adsorption.
+
Project support and advice
+
Dr. Cheng Li provided us tech supply and some competent cells, Top10.
+
Dr. Meng Wang provided us some competent cells, BL21 and DE3.
+
  
Lab support
+
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
Zhenwu Zhang, researcher of School of Biology Science and Engineering, provided Lab space and gave us some advice about Lab management.
+
14.0pt;mso-bidi-font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif'>Sensor
 +
system<o:p></o:p></span></p>
  
Difficult technique support
+
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
Kejie Chen, master of Fermentation field, provided us plasmid pKD46 and guidance for gene knockout.
+
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
Project advisor support
+
color:#333333'>Xiaomei Lin and Xunxun Luo buried themselves in this part. They
Junjie Zhang had made his best of advising our project and plan designing.
+
just made the device such as P<sub>tet</sub>+cjblue+ter. And also, Xiaomei Li
 +
had finished the measurement work with the help of Xiaojing Li. In addition,
 +
combining with the device of CsgA transport system, Li and Lin successfully
 +
tested the fluorescent protein and checked if the system worked.<o:p></o:p></span></p>
  
Wiki support
+
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
All the members of our team are supposed to be commended on their hard work about Wiki making.
+
14.0pt;mso-bidi-font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif'>Design
Presentation coaching
+
work<o:p></o:p></span></p>
Li Pan, Ying Lin and Yanrui Ye do this work.
+
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Yihong Gu, Shuoyu Chen, Jiahui Zhang and Yunan Lin had undertaken
 +
all the tasks of designing of the whole team. They did a lot work including
 +
logo, ppt templates, wiki and uniforms. Taking step by step, as a result of
 +
clarified division and efficient cooperation,</span><span lang=EN-US
 +
style='font-size:12.0pt;line-height:150%'>&nbsp;</span><span lang=EN-US
 +
style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>they sophisticatedly design uniforms and posters for the IGEM.
 +
And Manfredi helped modify the wiki content.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal><span lang=EN-US style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'>Modeling
 +
Part<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Junying Su is responsible for final determination of
 +
mathematical model for our project. He devotes to modeling the function of our
 +
engineering E.coli accurately. In addition, he writes Parameters Measuring and
 +
Data Processing Schemes for four parts, including MntA Transport Model, PcadA
 +
Strength Model, csgA Polymerization Model and csgA-ECn Adsorption Model. He
 +
also assists the members of experiment group to finish measuring work.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Xiaobin Huang is responsible for studying the mechanism of MntA
 +
transport. In order to find out a suitable mathematical model, he has also
 +
studied the mechanism of sodium potassium pump and calcium pump, which are also
 +
the members of the P-type family of adenosine<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>triphosphatases (ATPases). At the same time, he devotes to
 +
establishing the model of Ptet Strength. He has found out two available models
 +
in the previous iGEM work of Team EPF-Lausanne 2008 and Team UC Davis 2013 respectively.
 +
[1][2] Additionally, he works in data processing.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Chongyin Han is responsible for studying the mechanism of csgA
 +
polymerization. In order to find out a suitable mathematical model, he has
 +
studied an available model in the work of Team INSA-Lyon 2014. [3] Whether this
 +
model is useful for us, we need further verification.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Nana Pang is responsible for studying the mechanism of PcadA and
 +
merR mutant, and devotes to establishing the model of PcadA Strength. In order
 +
to figure out the accurate concentration of cadmium ion at which PcadA takes
 +
obvious effect, she analyzes the combination process of cadmium ion and merR.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Jing Liao is responsible for studying the mechanism of csgA-ECn
 +
adsorption. She devotes to working out the value of disassociation constant Kd
 +
with the available data. In addition, she tries to figure out the optimal value
 +
of csgA-ECn length n to improve the ability of cadmium ion adsorption.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=center style='text-align:center'><span lang=EN-US
 +
style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'>Project
 +
support and advice<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Dr. Cheng Li provided us tech supply and some competent cells,
 +
Top10.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Dr. Meng Wang provided us some competent cells, BL21 and DE3.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal><span lang=EN-US style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'><o:p>&nbsp;</o:p></span></p>
 +
 
 +
<p class=MsoNormal align=center style='text-align:center'><a name="OLE_LINK3"></a><a
 +
name="OLE_LINK2"></a><a name="OLE_LINK1"><span style='mso-bookmark:OLE_LINK2'><span
 +
style='mso-bookmark:OLE_LINK3'><span lang=EN-US style='font-size:16.0pt;
 +
font-family:"Arial Unicode MS",sans-serif'>Lab support<o:p></o:p></span></span></span></a></p>
 +
 
 +
<span style='mso-bookmark:OLE_LINK3'></span><span style='mso-bookmark:OLE_LINK2'></span><span
 +
style='mso-bookmark:OLE_LINK1'></span>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Zhenwu Zhang, researcher of School of Biology Science and
 +
Engineering, provided Lab space and gave us some advice about Lab management.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal><span lang=EN-US style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'><o:p>&nbsp;</o:p></span></p>
 +
 
 +
<p class=MsoNormal align=center style='text-align:center'><span lang=EN-US
 +
style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'>Difficult
 +
technique support<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Kejie Chen, master of Fermentation field, provided us plasmid
 +
pKD46 and guidance for gene knockout.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=center style='text-align:center'><span lang=EN-US
 +
style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'>Project
 +
advisor support<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Junjie Zhang had made his best of advising our project and plan
 +
designing.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal><span lang=EN-US style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'><o:p>&nbsp;</o:p></span></p>
 +
 
 +
<p class=MsoNormal align=center style='text-align:center'><span lang=EN-US
 +
style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'>Wiki support<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>All the members of our team are supposed to be commended on
 +
their hard work about Wiki making.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=center style='text-align:center'><span lang=EN-US
 +
style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'>Presentation
 +
coaching<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal><span lang=EN-US style='font-size:12.0pt;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Li Pan, Ying Lin and Yanrui Ye do this work.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal><span lang=EN-US style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'><o:p>&nbsp;</o:p></span></p>
 +
 
 +
<p class=MsoNormal align=center style='text-align:center'><span lang=EN-US
 +
style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif'>Human
 +
Practices support<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>In 2015, the Policy and Practice of SCUT team divide into 4
 +
parts including Economics and ethics, Visits, Syn-biology Study and Outdoors.
 +
The leader of the PP group is Minghui Deng.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Thank Prof. Shouqian Guo, head of Resources and Environment
 +
Institute, for providing guidance about economic sphere.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>Thank Dr. Li, a researcher who is good in heavy metal wastewater
 +
treatment of Shenyang Automation Institute, for giving us a lot of help about Modern
 +
wastewater treatment process.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal align=left style='text-align:left;line-height:150%'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial Unicode MS",sans-serif;
 +
color:#333333'>What’s more, it’s appreciated that Guangzhou Municipal
 +
Environmental Protection Bureau gave us a chance to visit their labs and
 +
explaination.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal><span lang=EN-US style='font-size:16.0pt;font-family:"Arial Unicode MS",sans-serif;
 +
color:red'>Thanks and acknowledgements for all other people involved in helping
 +
make a successful iGEM team<o:p></o:p></span></p>
  
Human Practices support
 
In 2015, the Policy and Practice of SCUT team divide into 4 parts including Economics and ethics, Visits, Syn-biology Study and Outdoors. The leader of the PP group is Minghui Deng.
 
Thank Prof. Shouqian Guo, head of Resources and Environment Institute, for providing guidance about economic sphere.
 
Thank Dr. Li, a researcher who is good in heavy metal wastewater treatment of Shenyang Automation Institute, for giving us a lot of help about Modern wastewater treatment process.
 
What’s more, it’s appreciated that Guangzhou Municipal Environmental Protection Bureau gave us a chance to visit their labs and explaination.
 
<p>
 
Thanks and acknowledgements for all other people involved in helping make a successful iGEM team
 
</p>
 
 
 
 
<div class="space"></div>
 
<div class="space"></div>

Revision as of 20:22, 16 September 2015

Team:SCUT/Attributions

Ruiming Xiao, Xiaojing Li and Jionghui Li managed CsgA transport part. Ruiming Xiao and Xiaojing Li was in charge of the construction of PcadA +CsgA-EC(n)+TetR+RFP+ter., Jionghui Lialso made efforts to assist both of them. Ruiming Xiao made brilliant contributions and devoted himself to testing the MerR/CadA operon using red fluorescent protein and he also helped Li to measure the AU of the RFP. As for Xiaojing Li, she was diligent and she made great efforts to construct different number of EC protein in order to find the best one. Also, because of deleting the wild CsgA will help efficiently express EC, So Minghui Deng and Yulong Zhang were responsible for the CsgA deletion.

Sensor system

Xiaomei Lin and Xunxun Luo buried themselves in this part. They just made the device such as Ptet+cjblue+ter. And also, Xiaomei Li had finished the measurement work with the help of Xiaojing Li. In addition, combining with the device of CsgA transport system, Li and Lin successfully tested the fluorescent protein and checked if the system worked.

Design work

Yihong Gu, Shuoyu Chen, Jiahui Zhang and Yunan Lin had undertaken all the tasks of designing of the whole team. They did a lot work including logo, ppt templates, wiki and uniforms. Taking step by step, as a result of clarified division and efficient cooperation, they sophisticatedly design uniforms and posters for the IGEM. And Manfredi helped modify the wiki content.

Modeling Part

Junying Su is responsible for final determination of mathematical model for our project. He devotes to modeling the function of our engineering E.coli accurately. In addition, he writes Parameters Measuring and Data Processing Schemes for four parts, including MntA Transport Model, PcadA Strength Model, csgA Polymerization Model and csgA-ECn Adsorption Model. He also assists the members of experiment group to finish measuring work.

Xiaobin Huang is responsible for studying the mechanism of MntA transport. In order to find out a suitable mathematical model, he has also studied the mechanism of sodium potassium pump and calcium pump, which are also the members of the P-type family of adenosine

triphosphatases (ATPases). At the same time, he devotes to establishing the model of Ptet Strength. He has found out two available models in the previous iGEM work of Team EPF-Lausanne 2008 and Team UC Davis 2013 respectively. [1][2] Additionally, he works in data processing.

Chongyin Han is responsible for studying the mechanism of csgA polymerization. In order to find out a suitable mathematical model, he has studied an available model in the work of Team INSA-Lyon 2014. [3] Whether this model is useful for us, we need further verification.

Nana Pang is responsible for studying the mechanism of PcadA and merR mutant, and devotes to establishing the model of PcadA Strength. In order to figure out the accurate concentration of cadmium ion at which PcadA takes obvious effect, she analyzes the combination process of cadmium ion and merR.

Jing Liao is responsible for studying the mechanism of csgA-ECn adsorption. She devotes to working out the value of disassociation constant Kd with the available data. In addition, she tries to figure out the optimal value of csgA-ECn length n to improve the ability of cadmium ion adsorption.

Project support and advice

Dr. Cheng Li provided us tech supply and some competent cells, Top10.

Dr. Meng Wang provided us some competent cells, BL21 and DE3.

 

Lab support

Zhenwu Zhang, researcher of School of Biology Science and Engineering, provided Lab space and gave us some advice about Lab management.

 

Difficult technique support

Kejie Chen, master of Fermentation field, provided us plasmid pKD46 and guidance for gene knockout.

Project advisor support

Junjie Zhang had made his best of advising our project and plan designing.

 

Wiki support

All the members of our team are supposed to be commended on their hard work about Wiki making.

Presentation coaching

Li Pan, Ying Lin and Yanrui Ye do this work.

 

Human Practices support

In 2015, the Policy and Practice of SCUT team divide into 4 parts including Economics and ethics, Visits, Syn-biology Study and Outdoors. The leader of the PP group is Minghui Deng.

Thank Prof. Shouqian Guo, head of Resources and Environment Institute, for providing guidance about economic sphere.

Thank Dr. Li, a researcher who is good in heavy metal wastewater treatment of Shenyang Automation Institute, for giving us a lot of help about Modern wastewater treatment process.

What’s more, it’s appreciated that Guangzhou Municipal Environmental Protection Bureau gave us a chance to visit their labs and explaination.

Thanks and acknowledgements for all other people involved in helping make a successful iGEM team

PROJECT PHOTOS

About Us

In 2015, we SCUT teams won top ten innovative and entrepreneurial team set up by SCUT. In our team everyone owns basic biological knowledge and skills of the competition(iGEM).

We are

Thanks

  • Zhang Zhenwu,Prof. Guo Shouqian,Dr. Li, Dr. Li Cheng,Dr. Wang Meng,Chen Kejie
  • Guangzhou Municipal Environmental Protection Bureau

COPYRIGHT ©2015-SCUT