Difference between revisions of "Team:Georgia State/Collaborations"
| Line 179: | Line 179: | ||
<div class="row text-center"> | <div class="row text-center"> | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
| − | <h3 class="h4" style="text-align: right;color: black;">Materials</h3> | + | <h3 class="h4" style="text-align: right;color: black;">Materials/Methods</h3> |
<div class="spacer-15"></div> | <div class="spacer-15"></div> | ||
<a class="cbox-gallary1 cboxElement" href="https://static.igem.org/mediawiki/2015/8/82/Blank_image_GSUiGEM.png" title="Image Title goes here"> | <a class="cbox-gallary1 cboxElement" href="https://static.igem.org/mediawiki/2015/8/82/Blank_image_GSUiGEM.png" title="Image Title goes here"> | ||
| Line 187: | Line 187: | ||
</a> | </a> | ||
<div> | <div> | ||
| − | <p class="lead" style="color: rgb(0, 0, 0);"> | + | <p class="lead" style="color: rgb(0, 0, 0);">We grew XL1-Blue E. coli cells in liquid culture of lab grade luria broth and J-broth. The media was either autoclaved or microwaved for 8 minutes at 650 Watts before the cells were added. </p> |
| − | + | <p>We then made competent cells out of XL1-Blue e. coli cells that were grown in each type of media following the Inoue method (Inoue, 1990) and calculated the transformation efficiency for comparison.</p> | |
| + | |||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 23:41, 16 September 2015
Collaborations
Backyard Biology @ Emory University: Project JBroth
Low Cost LB and Competent Cells!
Our interest in our topic began when we were researching possible projects. What we found most interesting was a research paper from the Journal of Pakistan Medical Association that tested the efficacy of microwaving LB rather than autoclaving. Also, a project that inspired us was the project from the University of Calgary (2014) that tested the efficacy of a homemade wash for competent cells. From these projects, we asked ourselves: Could one conduct a synthetic biology project without access to a professional laboratory?
This summer, we created a cost effective alternative to lab grade Luria Broth (LB). To compare our homemade broth, J-Broth, and lab grade LB we compared optical densities of liquid cultures and transformation efficiency of competent cells. For each method of testing, we compared autoclaved LB, microwaved LB, and microwaved J-Broth.
Materials/Methods
We grew XL1-Blue E. coli cells in liquid culture of lab grade luria broth and J-broth. The media was either autoclaved or microwaved for 8 minutes at 650 Watts before the cells were added.
We then made competent cells out of XL1-Blue e. coli cells that were grown in each type of media following the Inoue method (Inoue, 1990) and calculated the transformation efficiency for comparison.
Results