Difference between revisions of "Team:Brasil-USP/Notebook/Calendar"

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<h1 align=center><strong>Life Inside the LAB</strong></h1>
 
<h1 align=center><strong>Life Inside the LAB</strong></h1>
  
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<thead><h2 align=center>July</h2>
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<thead><h2 align=center>June</h2>
  
 
</thead>
 
</thead>
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     <th style="background-color:#bfacc0">Sunday</th>
 
     <th style="background-color:#bfacc0">Sunday</th>
 
   </tr> <tr>
 
   </tr> <tr>
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     <td>30/06
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     <td> 02/06
 
     <ul>
 
     <ul>
−
       <li><a href="https://static.igem.org/mediawiki/2014/7/74/PCR.pdf" style="color:#f05151">PCR</a> lasR (<a href="http://parts.igem.org/Part:BBa_C0079" style="color:#3ab473">BBa_C0079</a>). The purpose of this PCR was to add the RBS (<a href="http://parts.igem.org/Part:BBa_K143021" style="color:#3ab473">BBa_K143021</a>), through addition of the sequence in the primer foward, and also to remove the LVA tag. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this part will be called <a href="http://parts.igem.org/Part:BBa_K1521001" style="color:#3ab473">BBa_K1521001</a>.</li>
+
       <li> iGEM kit arrived! :D </li>
 
     </ul>
 
     </ul>
 
     </td>
 
     </td>

Revision as of 00:02, 17 September 2015

Calendar

Notebook

Life Inside the LAB

June

Monday Tuesday Wednesday Thursday Friday Saturday Sunday
02/06
  • iGEM kit arrived! :D
01/07 02/07 03/07 04/07 05/07 06/07
07/07 08/07 09/07 10/07
  • Miniprep, Quantification and Restriction Analysis:
    • BBa_B0015 (restriction analysis fail, repeat)
  • PCR the qteE gene for amplification. We also added the RBS (BBa_K143021,) using the foward primer. In our primers we only added the restriction sites X and S, so we still need to put it in the pSB1C3 for the Biobrick standard completion. When standardized this biobrick will be called BBa_K1521000.
  • Ligation of RBS+lasR in PTZ57R/T vector (instaclone kit)
 11/07 12/07 13/07
14/07 15/07 16/07 17/07 18/07 19/07 20/07
21/07 22/07 23/07 24/07 25/07 26/07 27/07
28/07
  • Assembly AIII:
    • Restriction analysis (+NdeI enzyme)
    • OBS: This analysis was reapeted because we had problems confirming the assembly. The issue was that both the construction and the pSB1C3 vector had similar size.
  • Assembly AII, AV and AVI:
 29/07 30/07 31/07
  • Assembly AII, AV and AVI:
    • Inoculum
  • PCR BBa_K143055 for RBS removal
  • OBS: This was one of the parts sent by the Imperial College Team. Because of a comunication failure we assumed that this part was comming without the RBS, so we included a RBS in the parts we were going to use with this Lac promotor :)
  • PCR of Plac BBa_K143055 was followed by a Gel Purification and Ligation in the PUC19 vector

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