Difference between revisions of "Team:Aalto-Helsinki/Experiments"

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<li><p><a href="https://static.igem.org/mediawiki/2015/a/ae/Aalto-Helsinki_purpald.pdf" target="_blank">Purpald assay</a></p></li>
 
<li><p><a href="https://static.igem.org/mediawiki/2015/a/ae/Aalto-Helsinki_purpald.pdf" target="_blank">Purpald assay</a></p></li>
 
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<h4>Kits</h4>
 
<h4>Kits</h4>
 
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<li><p>We used Thermo Fisher Scientific's kits for MiniPreps, PCR purification and Gel extraction. <span style="color:red">Tähän kittien tuotenumerot</span></p></li>
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<li><p>For plasmid extraction, we used both Termo Fisher Scientific's MiniPrep kit (<a href="https://www.thermofisher.com/order/catalog/product/K0502" style="padding:0" target="_blank">#K0691</a>) and Macherey-Nagel's Plasmid Easy Pure kit (<a href="http://www.mn-net.com/ProductsBioanalysis/DNAandRNApurification/PlasmidDNApurificationeasyfastreliable/NucleoSpinPlasmidEasyPure/tabid/1384/language/en-US/Default.aspx" target="_blank">740727.250</a>).<br/>
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For PCR purification and gel extraction we used Thermo Fisher Scientific's kits (<a href="https://www.thermofisher.com/order/catalog/product/K0701" style="padding:0" target="_blank">#K0502</a>, <a href="https://www.thermofisher.com/order/catalog/product/K0691" style="padding:0" target="_blank">#K0691</a>)</p></li>
 
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Latest revision as of 06:15, 17 September 2015

Protocols

You can find all of the protocols used in our project here as .pdf files. We based many of our protocols on what we found from research articles, openwetware.org, and other webpages. Some were given to us by 2014 Aalto-Helsinki team or researchers in Aalto University and VTT, the Techinical Research Center of Finland.

Preparations

Assembly

Measurements

Kits

  • For plasmid extraction, we used both Termo Fisher Scientific's MiniPrep kit (#K0691) and Macherey-Nagel's Plasmid Easy Pure kit (740727.250).
    For PCR purification and gel extraction we used Thermo Fisher Scientific's kits (#K0502, #K0691)