Difference between revisions of "Team:NJAU China"

Line 9: Line 9:
 
     <title>Home</title>
 
     <title>Home</title>
 
     <style type="text/css">
 
     <style type="text/css">
−
*{
+
    *{
−
padding:0;
+
/*margin: 0 auto;*/
−
text-decoration:none;
+
font-family: "Trebuchet MS", Arial, Helvetica, sans-serif;
−
margin:0;}
+
margin:0;
−
        body {
+
padding:0;
−
            padding-bottom: 40px;
+
}
−
            background:#ffa54f;
+
body{
−
            color: #ffffe0;
+
  background:red;
−
            font-size: 16px;
+
}
−
            font-family:Calibri;
+
#maintop{
−
        }
+
width:auto;
−
pre{
+
height:240px;
−
font-size:14px;
+
background:url(../images/NJAU_2015_navtop.jpg) no-repeat;
−
font-family:Calibri;}
+
background-size:100%;
−
        #myheader{
+
}
−
            width:1366px;
+
#box{
−
    height:500px;
+
margin: 0 auto;
−
    margin:0 auto;
+
}
−
    position:relative;
+
#pic{
−
    font-family:Arial, Helvetica, sans-serif;
+
height: 400px;
−
            background:#ffffff url("https://static.igem.org/mediawiki/2015/f/fa/NJAU_China01.png");;
+
}
−
        }
+
#pic #pic-left{
−
        #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {
+
width:25%;
−
            display: none;
+
height:400px;
−
        }
+
float:left;
 +
}
 +
#pic #pic-in{
 +
width:50%;
 +
height:350px;
 +
float:left;
 +
}
 +
video{
 +
display: block;
 +
width:100%;
 +
height:390px;
 +
margin-top:8px;
 +
color:#FFF;
 +
font-size:xx-large;
 +
}
 +
#pic #pic-right{
 +
width:25%;
 +
height:400px;
 +
float:left;
 +
}
 +
#pic img{
 +
width:100%;
 +
height:70%;
 +
}
 +
#block{
 +
margin:0 auto;
 +
height:900px;
 +
width:1200px;
 +
text-align:justify;
 +
font-family:Verdana, Geneva, sans-serif;
 +
}
 +
#block ul li{
 +
list-style: none;
 +
}
 +
.sub1,.sub2,.sub3,.sub4,.main1,.main2,.main3,.main4,.main5{
 +
width:33%;
 +
height:300px;
 +
}
 +
.sub1,.sub2,.sub3,.sub4{
 +
background: white;
 +
}
 +
.main1,.main2,.main4,.main5{
 +
background: #333;
 +
}
 +
.main3{
 +
background:url(../images/Njau_2015_mid1.jpg);
 +
background-size:100% 100%;
 +
}
 +
.sub1,.sub2,.main1{
 +
float: left;
 +
}
 +
.main2,.main3,.main4{
 +
float: left;
 +
}
 +
.sub3,.main5,.sub4{
 +
float: left;
 +
}
 +
.main1,.main2,.main3,.main4,.main5{
 +
font-family:;
 +
font-size: 13px;
 +
color:#FFFF99;
 +
word-wrap: break-word;
 +
text-indent: 10px;
 +
}
 +
.sub2{
 +
background: #33CC99;
 +
}
 +
.sub1,.sub4{
 +
background: #CC9933;
 +
}
 +
.sub3{
 +
background: #CCCC00;
 +
}
 +
span.f1,span.f2{
 +
display: block;
 +
overflow: hidden;
 +
padding:5px 10px;
 +
    width: auto;
 +
height:250px;
 +
}
 +
span.f1:hover{
 +
padding:5px 40px;
 +
-moz-transform: scale(1.2);
 +
-ms-transform: scale(1.2);
 +
-o-transform: scale(1.2);
 +
-webkit-transform: scale(1.2);
 +
color: #000;
 +
opacity: 0.8;
 +
background: #33CCCC;
 +
    transition: background 2s ease 0s;
 +
  -moz-transition: background 1.5s ease 0s;
 +
  -ms-transition: background 1.5s ease 0s;
 +
  -o-transition: background 1.5s ease 0s;
 +
  -webkit-transition: background 1.5s ease 0s;
 +
  transition: color 0.5s ease 0s;
 +
  -moz-transition: color 0.5s ease 0s;
 +
  -o-transition: color 0.5s ease 0s;
 +
  -ms-transition: color 0.5s ease 0s;
 +
  -webkit-transition: color 0.5s ease 0s;
 +
}
 +
span.f2:hover{
 +
padding:5px 40px;
 +
-moz-transform: scale(1.2);
 +
-ms-transform: scale(1.2);
 +
-o-transform: scale(1.2);
 +
-webkit-transform: scale(1.2);
 +
color:#066;
 +
opacity: 0.8;
 +
background: #66FF00;
 +
    transition: background 2s ease 0s;
 +
  -moz-transition: background 2s ease 0s;
 +
  -ms-transition: background 2s ease 0s;
 +
  -o-transition: background 2s ease 0s;
 +
  -webkit-transition: background 2s ease 0s;
 +
  transition: color 1s ease 0s;
 +
  -moz-transition: color 1s ease 0s;
 +
  -o-transition: color 1s ease 0s;
 +
  -ms-transition: color 1s ease 0s;
 +
  -webkit-transition: color 1s ease 0s;
 +
}
 +
.main3:hover{
 +
background:url(../images/Njau_2015_mid2.jpg);
 +
background-size:100% 100%;
 +
}
 +
#footer{
 +
width: auto;
 +
height: 111px;
 +
    background:#999966;
 +
    margin: 0 auto;
 +
}
 +
#footer img{
 +
margin: 10px 0 10px 100px;
 +
display: inline;
 +
float: left;
 +
}
  
−
        /*-- hides default wiki settings --*/
+
body {
−
     
+
/*font-family:Tahoma, Geneva, sans-serif;*/
 +
font-family:"Trebuchet MS", Arial, Helvetica, sans-serif;
 +
font-size:1em;
 +
margin:0 auto;
 +
}
 +
.container {
 +
    left:0;
 +
width:auto;
 +
    background-color:#252525;
 +
    padding:0 100px;
 +
position:relative;
 +
}
  
−
        h1, h2, h3, h4, h5 {
+
/* general styles */
−
            font-family: Georgia, Times, "Calibri", serif;
+
.menu, .menu ul {
−
        }
+
    list-style: none;
 +
width:100%;
 +
    margin:0 auto;
 +
}
 +
.menu {
 +
    height: 58px;
 +
}
 +
.menu li {
 +
    background: -moz-linear-gradient(#292929, #252525);
 +
    background: -ms-linear-gradient(#292929, #252525);
 +
    background: -webkit-gradient(linear, left top, left bottom, color-stop(0%, #292929), color-stop(100%, #252525));
 +
    background: -webkit-linear-gradient(#292929, #252525);
 +
    background: -o-linear-gradient(#292929, #252525);
 +
    filter: progid:DXImageTransform.Microsoft.gradient(startColorstr='#292929', endColorstr='#252525');
 +
    -ms-filter: "progid:DXImageTransform.Microsoft.gradient(startColorstr='#292929', endColorstr='#252525')";
 +
    background: linear-gradient(#292929, #252525);
 +
    border-bottom: 2px solid #181818;
 +
    border-top: 2px solid #303030;
 +
    min-width:100px;
 +
   
 +
}
 +
.menu li img{
 +
display:block;
 +
width:60px;
 +
height:54px;
 +
margin:0;
 +
}
 +
.menu > li {
 +
    display: block;
 +
    float: left;
 +
    position: relative;
 +
}
 +
.menu a {
 +
    border-left: 3px solid rgba(0, 0, 0, 0);
 +
    color: #FFF;
 +
    display: block;
 +
    font-family:Arial, Helvetica, sans-serif;
 +
    font-size: 15px;
 +
font-weight:bold;
 +
    line-height: 54px;
 +
    padding: 0 30px;
 +
    text-decoration: none;
 +
}
  
−
        #top-section {
+
/* onhover styles */
−
            /*-- styling for default menu bar (edit, page, history, etc.) --*/
+
.menu li:hover {
−
            background-color: #ffffe0;
+
    background-color: #1c1c1c;
−
            border: 0 none;
+
    background: -moz-linear-gradient(#1c1c1c, #1b1b1b);
−
            height: 14px;
+
    background: -ms-linear-gradient(#1c1c1c, #1b1b1b);
−
            z-index: 100;
+
    background: -webkit-gradient(linear, left top, left bottom, color-stop(0%, #1c1c1c), color-stop(100%, #1b1b1b));
−
            top: 0;
+
    background: -webkit-linear-gradient(#1c1c1c, #1b1b1b);
−
            position: fixed;
+
    background: -o-linear-gradient(#1c1c1c, #1b1b1b);
−
            width: 975px;
+
    filter: progid:DXImageTransform.Microsoft.gradient(startColorstr='#1c1c1c', endColorstr='#1b1b1b');
−
            left: 50%;
+
    -ms-filter: "progid:DXImageTransform.Microsoft.gradient(startColorstr='#1c1c1c', endColorstr='#1b1b1b')";
−
            margin-left: -487px;
+
    background: linear-gradient(#1c1c1c, #1b1b1b);
−
        }
+
    border-bottom: 2px solid #222222;
−
.firstHeading {
+
    border-top: 2px solid #1B1B1B;
−
            width: 975px;
+
}
−
            margin: 0px auto;
+
.menu li:hover > a {
−
            padding-top: 100px;
+
    border-radius: 5px 0 0 0;
−
            margin-bottom: 20px;display:none;
+
    border-left: 3px solid #369;
−
            font-family: Georgia, Times, "Calibri", serif;
+
    color:#369;
−
        }
+
}
−
        #top-section-bar {
+
−
            /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
+
−
            background-color: #ffffe0;
+
−
            height: 14px;
+
−
            display: block;
+
−
            z-index: 10;
+
−
            position: fixed;
+
−
            width: 100%;
+
−
            top: 0;
+
−
        }
+
  
−
        body {
+
/* submenu styles */
−
            background-color: #f60;
+
.submenu {
−
        }
+
    left: 0;
 +
    max-height: 0;
 +
    position: absolute;
 +
    top: 100%;
 +
    z-index: 0;
 +
min-width:220px;
 +
    -webkit-perspective: 400px;
 +
    -moz-perspective: 400px;
 +
    -ms-perspective: 400px;
 +
    -o-perspective: 400px;
 +
    perspective: 400px;
 +
 +
}
 +
.submenu li {
 +
    opacity: 0;
 +
    -webkit-transform: rotateY(90deg);
 +
    -moz-transform: rotateY(90deg);
 +
    -ms-transform: rotateY(90deg);
 +
    -o-transform: rotateY(90deg);
 +
    transform: rotateY(90deg);
  
−
        #globalWrapper, #content {
+
    -webkit-transition: opacity .4s, -webkit-transform .5s;
−
            /*-- changes default wiki settings --*/
+
    -moz-transition: opacity .4s, -moz-transform .5s;
−
            width: 100%;
+
    -ms-transition: opacity .4s, -ms-transform .5s;
−
            height: 100%;
+
    -o-transition: opacity .4s, -o-transform .5s;
−
            border: 0px;
+
    transition: opacity .4s, transform .5s;
−
            background-color: transparent;
+
}
−
            margin: 0px;
+
.menu .submenu li:hover a {
−
            padding: 0px;
+
    border-left: 3px solid #454545;
−
        }
+
    border-radius: 0;
 +
    color: #69F;
 +
}
 +
.menu > li:hover .submenu, .menu > li:focus .submenu {
 +
    max-height: 2000px;
 +
    z-index: 10;
 +
}
 +
.menu > li:hover .submenu li, .menu > li:focus .submenu li {
 +
    opacity: 1;
 +
    -webkit-transform: none;
 +
    -moz-transform: none;
 +
    -ms-transform: none;
 +
    -o-transform: none;
 +
    transform: none;
 +
}
  
−
        html, body, .wrapper {
+
/* CSS3 delays for transition effects */
−
            /*-- changes default wiki settings --*/
+
.menu li:hover .submenu li:nth-child(1) {
−
            width: 100%;
+
    -webkit-transition-delay: 0s;
−
            height: 100%;
+
    -moz-transition-delay: 0s;
−
            background-color: transparent;
+
    -ms-transition-delay: 0s;
−
        }
+
    -o-transition-delay: 0s;
−
 
+
    transition-delay: 0s;
−
        #container {
+
}
−
            /*-- creates container for all content on page --*/
+
.menu li:hover .submenu li:nth-child(2) {
−
            font-family: Arial, Helvetica, sans-serif;
+
    -webkit-transition-delay: 50ms;
−
            font-weight: normal;
+
    -moz-transition-delay: 50ms;
−
            font-size: 14px;
+
    -ms-transition-delay: 50ms;
−
            color: #ffa54f;
+
    -o-transition-delay: 50ms;
−
            width: 960px;
+
    transition-delay: 50ms;
−
            margin-left: auto;
+
}
−
            margin-right: auto;
+
.menu li:hover .submenu li:nth-child(3) {
−
            background-color: #transparent;
+
    -webkit-transition-delay: 100ms;
−
            margin-top: 0px;
+
    -moz-transition-delay: 100ms;
−
        }
+
    -ms-transition-delay: 100ms;
−
 
+
    -o-transition-delay: 100ms;
−
        .container {
+
    transition-delay: 100ms;
−
            font-size: 14px;
+
}
−
            width:700px;
+
.menu li:hover .submenu li:nth-child(4) {
−
            float:right;
+
    -webkit-transition-delay: 150ms;
−
        }
+
    -moz-transition-delay: 150ms;
 +
    -ms-transition-delay: 150ms;
 +
    -o-transition-delay: 150ms;
 +
    transition-delay: 150ms;
 +
}
 +
.menu li:hover .submenu li:nth-child(5) {
 +
    -webkit-transition-delay: 200ms;
 +
    -moz-transition-delay: 200ms;
 +
    -ms-transition-delay: 200ms;
 +
    -o-transition-delay: 200ms;
 +
    transition-delay: 200ms;
 +
}
 +
.menu li:hover .submenu li:nth-child(6) {
 +
    -webkit-transition-delay: 250ms;
 +
    -moz-transition-delay: 250ms;
 +
    -ms-transition-delay: 250ms;
 +
    -o-transition-delay: 250ms;
 +
    transition-delay: 250ms;
 +
}
 +
.menu li:hover .submenu li:nth-child(7) {
 +
    -webkit-transition-delay: 300ms;
 +
    -moz-transition-delay: 300ms;
 +
    -ms-transition-delay: 300ms;
 +
    -o-transition-delay: 300ms;
 +
    transition-delay: 300ms;
 +
}
 +
.menu li:hover .submenu li:nth-child(8) {
 +
    -webkit-transition-delay: 350ms;
 +
    -moz-transition-delay: 350ms;
 +
    -ms-transition-delay: 350ms;
 +
    -o-transition-delay: 350ms;
 +
    transition-delay: 350ms;
 +
}
  
−
        .buttontext {
+
.submenu li:nth-child(1) {
−
            color: #ffa54f
+
    -webkit-transition-delay: 350ms;
−
        }
+
    -moz-transition-delay: 350ms;
−
#my_h1{position:absolute;top:70px;left:170px;border:none;}
+
    -ms-transition-delay: 350ms;
−
#my_h1 a{display:block;width:190px;height:130px;text-indent:-9999px;}
+
    -o-transition-delay: 350ms;
−
#my_nav{position:absolute;top:402px;left:200px;width:990px;height:50px;}
+
    transition-delay: 350ms;
−
#my_nav ul{width:100%;margin:0;padding:0;list-style:none;font-size:24px;}
+
−
#my_nav ul li{display::inline-block;padding:9px 30px;float:left;list-style:none;position:relative;}
+
−
#my_nav ul li:hover{background:#ffec8b;}#my_nav ul li a{color:#ffc125;text-decoration:none;}#project{display:none;position:absolute;z-index:100;top:47px;left:0px;background:#CF6;width:100px;}#project dd{width:135px;font-size:16px;
+
−
background:#fff68f;height:40px;margin:0;}#project dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#project dd a:hover{background:#fffacd;}#achievement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#achievement dd{width:198px;font-size:16px;background:#fff68f;height:40px;margin:0;}#achievement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#achievement dd a:hover{background:#fffacd;}#prctice{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#prctice dd{width:300px;font-size:16px;background:#fff68f;height:40px;margin:0;}#prctice dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#prctice dd a:hover{background:#fffacd;}#ackonwledgement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#ackonwledgement dd{width:257px;font-size:16px;background:#fff68f;height:40px;margin:0;}#ackonwledgement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#ackonwledgement dd a:hover{backgrounda:#fffacd;}
+
−
#my_content{
+
−
width:1030px;
+
−
margin:0px auto;}
+
−
#my_abstract{
+
−
width:300px;
+
−
margin:0 0 0 25px;
+
−
        float:left;
+
−
}
+
−
#my_abstract h2{
+
−
height:50px;
+
−
line-height:50px;
+
−
text-indent:50px;
+
−
border:none;
+
−
background:#999;
+
−
margin:0 0 20px 0;
+
−
padding:0;
+
 
}
 
}
−
#my_abstract p{
+
.submenu li:nth-child(2) {
−
text-indent:30px;
+
    -webkit-transition-delay: 300ms;
−
height:240px;
+
    -moz-transition-delay: 300ms;
−
overflow-y:scroll;
+
    -ms-transition-delay: 300ms;
 +
    -o-transition-delay: 300ms;
 +
    transition-delay: 300ms;
 
}
 
}
−
#my_contents{
+
.submenu li:nth-child(3) {
−
width:1030px;
+
    -webkit-transition-delay: 250ms;
−
position:relative;
+
    -moz-transition-delay: 250ms;
−
margin:0 auto;
+
    -ms-transition-delay: 250ms;
 +
    -o-transition-delay: 250ms;
 +
    transition-delay: 250ms;
 +
}
 +
.submenu li:nth-child(4) {
 +
    -webkit-transition-delay: 200ms;
 +
    -moz-transition-delay: 200ms;
 +
    -ms-transition-delay: 200ms;
 +
    -o-transition-delay: 200ms;
 +
    transition-delay: 200ms;
 +
}
 +
.submenu li:nth-child(5) {
 +
    -webkit-transition-delay: 150ms;
 +
    -moz-transition-delay: 150ms;
 +
    -ms-transition-delay: 150ms;
 +
    -o-transition-delay: 150ms;
 +
    transition-delay: 150ms;
 +
}
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    <h1>Project Description</h1>
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    <p>The conversion of transient information into long-lasting responses is a common aspect of many biological processes and is crucial for the design of sophisticated synthetic circuits <sup> [1] </sup>. Whereas, the most cellular memories are limited to protein levels currently, which are transient, instantaneous and unapparent. To overcome these shortcuts, this year we design a system to achieve the storage of information by a transient stimulation but use a long-lasting response.</p>  
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<source src="video/thehorcorx.mp4" type=video/mp4 />
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    <p>In this project, we try to complete a simulation of memorizer. The two processes are divided into separated bacterial strains to play as the “Recorder” and the “Saver”. The system is designed to be activated and deactivated via light- regulated fusion proteins <sup> [2] </sup>, which then active protein synthesis to complete the process. Once the system is activated by simulation of blue light, the information storage would be accomplished by conjugation, which cannot be carried out in usual time because of the specific gene deletion. When the memory needs to be erased, the CRISPR-Cas9 system in the “Saver” would be activated by the red light.</p>  
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  <p>By design such a cellular memory technology, we make a bacterial memory storage device. And together with biological transistor and other bio-electronic component, we can preview the prototype of the biological computer.</p>
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    <h3>Refrences:</h3>
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    <p>[1] Farzadfard F, Lu T K. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations[J]. science, 2014, 346(6211): 1256272.</p>
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    <p>[2] Möglich A, Ayers R A, Moffat K. Design and signaling mechanism of light-regulated histidine kinases[J]. Journal of molecular biology, 2009, 385(5): 1433-1444.</p>
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<div class="main1"><h3>introduction</h3><span class="f1"> The conversion of transient information into long-lasting responses is a common aspect of many biological processes and is crucial for the design of sophisticated synthetic circuits. Whereas, the most cellular memories are limited to protein levels currently, which are transient, instantaneous and unapparent. To overcome these shortcuts, this year we design a system to achieve the storage of information by a transient stimulation but use a long-lasting response. Because of the similarity with the process of memory, we call it “the horcrux”. </span></div>
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<div class="sub2"><img src="https://static.igem.org/mediawiki/2015/5/54/NJAU_China2015_subblock2.png" alt="pics"></div>
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    <div class="main2"><h3>our goals</h3><span class="f2">This year we want to design a system which can respond to external stimuli and then maintain a lasting signal. To make it come true, we need to design a sophisticated gene circuits. We try to use two different strains of E.coli to be our “Recorder” and “Saver”. We aim to finish the plasmid construction and functional verification, then use GFP as a report gene to get some data so we can build a mathematical model for our system and predict the effect.
 +
<br />In order to enable our biological system to be able to handle data processing with computer information, our students want to design a device for our bacterial system to validate system functionality and measure and collect data. Furthermore, we want it to be widely applicable to the biological research.
  
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</span></div>
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<div class="main3"></div>
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<div class="main4"><h3>accomplishment</h3><span class="f1">
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<b>-biological experiment</b><br />
 +
We tried our best to construct the plasmid, made the functional verification, got some data and established the mathematical model.<br />
 +
<b>-device assembly</b><br />
 +
We have designed a set of simple starting and measuring devices for our biological system, and it will be used in our prototype design.<br />
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<b>-human practice</b><br />
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We conducted a wide range of “virtual interviews” for our subject areas. We have also exchanged our ideas and carried out some cooperation with some teams.<br />
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<div class="main5"><span class="f2">introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce</span></div>
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<div class="sub4"><img src="https://static.igem.org/mediawiki/2015/b/b5/NJAU_2015_subblock4_home6.png" alt="pics"></div>
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Revision as of 16:42, 17 September 2015

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  • pics

    introduction

    The conversion of transient information into long-lasting responses is a common aspect of many biological processes and is crucial for the design of sophisticated synthetic circuits. Whereas, the most cellular memories are limited to protein levels currently, which are transient, instantaneous and unapparent. To overcome these shortcuts, this year we design a system to achieve the storage of information by a transient stimulation but use a long-lasting response. Because of the similarity with the process of memory, we call it “the horcrux”.
    pics

  • our goals

    This year we want to design a system which can respond to external stimuli and then maintain a lasting signal. To make it come true, we need to design a sophisticated gene circuits. We try to use two different strains of E.coli to be our “Recorder” and “Saver”. We aim to finish the plasmid construction and functional verification, then use GFP as a report gene to get some data so we can build a mathematical model for our system and predict the effect.
    In order to enable our biological system to be able to handle data processing with computer information, our students want to design a device for our bacterial system to validate system functionality and measure and collect data. Furthermore, we want it to be widely applicable to the biological research.

    accomplishment

    -biological experiment
    We tried our best to construct the plasmid, made the functional verification, got some data and established the mathematical model.
    -device assembly
    We have designed a set of simple starting and measuring devices for our biological system, and it will be used in our prototype design.
    -human practice
    We conducted a wide range of “virtual interviews” for our subject areas. We have also exchanged our ideas and carried out some cooperation with some teams.
  • pics
    introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce introduce
    pics
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