Difference between revisions of "Team:Cooper Union/Experiments"

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<h2>Experiments &amp; Protocols</h2>
 
<h2>Experiments &amp; Protocols</h2>
  
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<p>Describe the experiments, research and protocols you used in your iGEM project.</p>
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<h4>Bacteria Media w/ agar (solid media)</h4>
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<h5>Materials</h5>
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<li>500mL bottle</li>
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<li>Deionized Water</li>
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<li>Agar</li>
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<li>LB Broth</li>
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<li>Chloramphenicol</li>
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<li>Yeast Extract</li>
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<li>Bacto Peptone</li>
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<ol type=”1”>
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<li>Add 250mL of deionized water to the 500mL bottle. </li>
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<li>Weigh out 3.625g of agar using the weighing boat and add it to the 250mL of water.</li>
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<li>Weigh out 5.0g of LB Broth using the weighing boat and add it to the 250mL of water.</li>
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<li>Screw lid on bottle loosely.</li>
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<li>Place autoclave tape on the lid of the bottle.</li>
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<li>Place tray with approximately 1cm of water in height into the autoclave. </li>
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<li>Place 500mL bottle into autoclave with "liquid" selected and press "start".</li>
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<li>After an hour, remove bottle from autoclave. Let bottle sit for approximately 30 minutes.</li>
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<li>Add 370uL of chloramphenicol to the 250mL solution.</li>
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<li>Pour solution into petri dishes, covering the bottom of the dish.</li>
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<li>Place two stripes on each dish.</li>
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<li>Let dishes sit overnight.</li>
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</ol>
  
 
<h5>What should this page contain?</h5>
 
<h5>What should this page contain?</h5>

Revision as of 02:11, 18 September 2015

Cooper Union 2015 iGEM



Experiments & Protocols

Bacteria Media w/ agar (solid media)

Materials
  • 500mL bottle
  • Deionized Water
  • Agar
  • LB Broth
  • Chloramphenicol
  • Yeast Extract
  • Bacto Peptone
    1. Add 250mL of deionized water to the 500mL bottle.
    2. Weigh out 3.625g of agar using the weighing boat and add it to the 250mL of water.
    3. Weigh out 5.0g of LB Broth using the weighing boat and add it to the 250mL of water.
    4. Screw lid on bottle loosely.
    5. Place autoclave tape on the lid of the bottle.
    6. Place tray with approximately 1cm of water in height into the autoclave.
    7. Place 500mL bottle into autoclave with "liquid" selected and press "start".
    8. After an hour, remove bottle from autoclave. Let bottle sit for approximately 30 minutes.
    9. Add 370uL of chloramphenicol to the 250mL solution.
    10. Pour solution into petri dishes, covering the bottom of the dish.
    11. Place two stripes on each dish.
    12. Let dishes sit overnight.
    What should this page contain?
    • Protocols
    • Experiments
    • Documentation of the development of your project

    Inspiration