Difference between revisions of "Team:CHINA CD UESTC/Journal"

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<div class="exhib_date_right">April 27th</div>
 
<div class="exhib_date_right">April 27th</div>
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<p>Ascertained the origin of laccase gene - derived from <i>Escherichia coli</i>.</p>
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<p>Ascertained the origin of <i>laccase</i> gene - derived from <i>Escherichia coli</i>.</p>
 
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<div class="exhib_date_left">May 5th</div>
 
<div class="exhib_date_left">May 5th</div>
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<p>Designed the primers of mamW, RFP and laccase.</p>
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<p>Designed the primers of <i>mamW</i>, <i>RFP</i> and <i>laccase</i>.</p>
 
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<div class="exhib_date_right">June 15th</div>
 
<div class="exhib_date_right">June 15th</div>
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<p>Linearization of the pACYCDuet-1 and amplification of the mamW, RFP and laccase.</p>
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<p>Linearization of the pACYCDuet-1 and amplification of the <i>mamW</i>, <i>RFP</i> and <i>laccase</i>.</p>
 
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<div class="exhib_date_right">July 27th</div>
 
<div class="exhib_date_right">July 27th</div>
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<p>We transferred mamW, RFP and laccase gene into <i>BL21</i> and induced its expression.</p>
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<p>We transferred <i>mamW</i>, <i>RFP</i> and <i>laccase</i> gene into <i>BL21</i> and induced its expression.</p>
 
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<div class="exhib_date_right">August 5th</div>
 
<div class="exhib_date_right">August 5th</div>
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<p>Amplified the mamXY with PCR from <i>MSR-1</i>. Completed the construction of vector piGEM-pET28a-mamAB.</p>
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<p>Amplified the <i>mamXY</i> with PCR from <i>MSR-1</i>. Completed the construction of vector piGEM-AB.</p>
 
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<div class="exhib_date_right">August 23th</div>
 
<div class="exhib_date_right">August 23th</div>
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<p>Connected mamXY with the vector pCDFDuet-1 and connected mamGFDC + mms6 with the vector pCDFDuet-1-mamXY.</p>
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<p>Connected <i>mamXY</i> with the vector pCDFDuet-1 and connected <i>mamGFDC</i> + <i>mms6</i> with the vector pCDFDuet-1-mamXY.</p>
 
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<div class="exhib_date_right">August 29th</div>
 
<div class="exhib_date_right">August 29th</div>
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<p>We were excited to obtain the exact Parts including the mamW gene and mutanted it.</p>
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<p>We were excited to obtain the exact Parts including the <i>mamW</i> gene and mutanted it.</p>
 
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Revision as of 09:07, 18 September 2015

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JOURNAL

  Hi! Here recorded the “significant” stages of our team which we experienced throughout the whole summer. In the initial establishment of our team, we organized various social activities and uneasy demonstrated the project at the first time, which all presents to you here.

Journal

March 24th

The establishment of our team!

March 26th

Came up with gas detector that examine institution and several diseases of our body.

March 28th

Put forward a kind of novel plant paint which can paint on the plant surface to absorb PM2.5.

April 1st

Catch the idea of yeast probiotic yogurt which helps people who allergic to alcohol to rapid degradation of ethanol.

April 6th

We had a brain storming: tracer showing the concentration of biomolecules, plants can emit colored light, new type of bacterial cell, and wastewater treatment by magnetotactic bacterium, etc.

April 9th

We established the iGEM club in our campus.

April 20th

Determined the project as "magnetotactic E.coli generates electronics."

April 22th

Ascertained the origin of mamW gene in MSR-1 genome.

April 27th

Ascertained the origin of laccase gene - derived from Escherichia coli.

May 5th

Designed the primers of mamW, RFP and laccase.

May 7th

Purchased the laboratory consumables and prepared for the first human practice activity.

May 8th

Communicated with professor Hu from Hong Kong University Medicine School.

May 10th

Determined three vectors for co-transferred which are pACYCDuet-1, pET28a and pCDFDuet-1.

June 12th

Amplified the mamGFDC and mms6 with PCR from MSR-1.

June 15th

Linearization of the pACYCDuet-1 and amplification of the mamW, RFP and laccase.

June 18th

Presentation and science lesson at the experimental Kindergarden Affiliated to UESTC.

June 19th

Presentation and Interaction at the Primary School Affiliated to UESTC

July 15th

Four teaching teams’ from iGEM club departure towards schools in remote area.

July 21th

Our summer vocation began, in order to have fun before the summer iGEM training, we go out for an outing!

July 25th

Screened positive clones of mamW + RFP + laccase and RFP + laccase.

July 21th

The start of the Summer Training Camp.

July 25th

The establishment of the iGEM Southwest Union of China, also the first meet up.

July 27th

We transferred mamW, RFP and laccase gene into BL21 and induced its expression.

July 30th

Connected mamAB with the vector pET28a and complete the construction of vector piGEM-pET28a-mamAB.

August 5th

Amplified the mamXY with PCR from MSR-1. Completed the construction of vector piGEM-AB.

August 12th

Communication with technical expert at Anno Medical Devices Co.Ltd.

August 13th

Detection of laccase activity using by the ABTS. Communication with Manager Zhiyong Xu at Jin Douyun Space Company.

August 17th

Finished the project video.

August 23th

Connected mamXY with the vector pCDFDuet-1 and connected mamGFDC + mms6 with the vector pCDFDuet-1-mamXY.

August 25th

We visited Chengdu Biology Maker Space and got their technical support.

August 29th

We were excited to obtain the exact Parts including the mamW gene and mutanted it.

August 30th

We completed our enzymatic biofuel cell! It worked as we expected!

September 2nd

The first time we presented our whole project presentation in front of all instructors and professors of our school.

September 10th

It is an exciting day that we sent our Parts to MIT, wish the little box bon voyage. Happy to complete the construction of cell device and start to test it.

September 15th

Team members gathered together to modify the wiki for the finalist version under the guidance of our instructors.

September 18th

We completed our wiki and poster, which means we completed the basic standards of medals. Next is the comprehensive preparation of presentation.

September 23th

Set out for 2015 iGEM Giant Jamboree in MIT. We are coming, we are CHINA_CD_UESTC!