Difference between revisions of "Team:CHINA CD UESTC/Journal"

 
(32 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 
{{Template:Team:CHINA_CD_UESTC/menu}}
 
{{Template:Team:CHINA_CD_UESTC/menu}}
 +
{{Template:Team:CHINA_CD_UESTC/cssstyle}}
  
 
<!DOCTYPE html>
 
<!DOCTYPE html>
−
<!-- saved from url=(0028)http://www.whitefrontier.ch/ -->
+
<html>
−
<html lang="en" class="js flexbox canvas canvastext webgl no-touch geolocation postmessage websqldatabase indexeddb hashchange history draganddrop websockets rgba hsla multiplebgs backgroundsize borderimage borderradius boxshadow textshadow opacity cssanimations csscolumns cssgradients cssreflections csstransforms csstransforms3d csstransitions fontface generatedcontent video audio localstorage sessionstorage webworkers applicationcache svg inlinesvg smil svgclippaths wf-brandongrotesque-i4-active wf-brandongrotesque-i7-active wf-brandongrotesque-n4-active wf-brandongrotesque-n7-active wf-dincondensedweb-n4-active wf-freightbigpro-i4-active wf-freightbigpro-i7-active wf-freightbigpro-n4-active wf-freightbigpro-n7-active wf-active">
+
 
<head>
 
<head>
  
−
<link rel="stylesheet" href="https://2015.igem.org/Team:CHINA_CD_UESTC/Template:RightStyle.css?action=raw&ctype=text/css" type="text/css" />
 
 
</head>
 
</head>
 
<style type="text/css">
 
<style type="text/css">
Line 32: Line 31:
  
 
}
 
}
−
/*************************************************
 
−
first title
 
−
***********************************************/
 
−
#title {
 
−
    position: fixed;
 
−
font-size: 80px;
 
−
left:130px;
 
−
z-index: 5;
 
  
−
}
 
−
#firstTitle {
 
−
font-family:  Arial, Helvetica, sans-serif;
 
−
    position: fixed;
 
−
margin-top:0;
 
−
    margin-bottom:0;
 
−
left:50%;
 
−
top: 60px;
 
−
font-size: 80px;
 
−
color: #fdf7c1;
 
−
text-align: center;
 
−
overflow: hidden;
 
−
 
−
}
 
−
 
−
 
−
/*************************************************
 
−
right text frame container
 
−
***********************************************/
 
−
#RightContent {
 
−
float: right;
 
−
position: fixed;
 
−
left: 260px;
 
−
top: 0px;
 
−
right: 0;
 
−
height:100%;
 
−
overflow-y: scroll;
 
−
transition: all 200ms ease-out;
 
−
transform: translate3d(0, 0, 0);
 
−
z-index:5;
 
−
 
−
}
 
−
.transparent_class  {
 
−
border-radius: 0px;
 
−
position: absolute;
 
−
top: 350px;
 
−
left: 50px;
 
−
right: 50px;
 
−
background-color: #0072E3;
 
−
color:#000000;
 
−
filter:alpha(opacity=80);
 
−
-moz-opacity:0.8;
 
−
-khtml-opacity: 0.8;
 
−
opacity: 0.8;
 
−
}
 
−
.blockWords{
 
−
font-family:Arial, Helvetica, sans-serif;
 
−
font-size: 17px;
 
−
padding: 15px;
 
−
color: #ffffff;
 
−
}
 
−
/*************************************************
 
−
testtext
 
−
***********************************************/
 
−
#RightContentText {
 
−
position: absolute;
 
−
top: 510px;
 
−
left:18px;
 
−
right:18px;
 
−
background:#FFFFFF;
 
−
color: #000000;
 
−
transition: all 200ms ease-out;
 
−
transform: translate3d(0, 0, 0);
 
−
}
 
−
 
−
@media (min-width: 540px) {
 
−
  #firstTitle {font-size: 60px;}
 
−
}
 
−
@media (min-width: 768px) {
 
−
 
−
  #firstTitle {font-size: 60px;}
 
−
 
−
}
 
−
 
−
 
−
@media (min-width: 992px) {
 
−
 
−
  #firstTitle {font-size: 60px;}
 
−
 
−
}
 
−
 
−
@media (min-width: 1200px) {
 
−
  #firstTitle {font-size: 80px;}
 
−
 
−
  }
 
−
#content{
 
−
height:90px;
 
−
  background:transparent;
 
−
 
−
}
 
 
</style>
 
</style>
 
<body id="homeIndexBody">
 
<body id="homeIndexBody">
Line 142: Line 43:
 
<div id="RightContent">
 
<div id="RightContent">
 
<div class="transparent_class ">
 
<div class="transparent_class ">
−
<p class="blockWords">&nbsp;&nbsp;We are a skillful and persistent group of nine Finns. We started as a group of students who didn't really know each other, assuming that we were going to spend our summer studying synthetic biology with strange colleagues. In the end we got a bunch of new friends and (in addition to studying synthetic biology) we just might have spent one of the best summers of our lives.</p>
+
<p class="blockWords">&nbsp;&nbsp;Hi! Here recorded the “significant” stages of our team which we experienced throughout the whole summer. In the initial establishment of our team, we organized various social activities and uneasy demonstrated the project at the first time, which all presents to you here.</p>
 
</div>
 
</div>
  
Line 160: Line 61:
 
<div class="exhib_block">
 
<div class="exhib_block">
 
<div class="exhib_line_center"></div>
 
<div class="exhib_line_center"></div>
−
<div class="exhib_date_top">2012</div>
 
−
<p class="exhib_p_top">“Etiam gravida convallis, Columbus College, Columbus, Ohio</p>
 
 
 
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">March 24th</div>
 +
<p>The establishment of our team!</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
<div class="exhib_cont fright">
 
<div class="exhib_cont fright">
−
<div class="exhib_date_left">2010</div>
+
<div class="exhib_date_left">March 26th</div>
−
<p>“TEtiam nunc tortor”, Junction View Studios, Columbus, Ohio</p>
+
<p>Came up with gas detector that examine institution and several diseases of our body.</p>
−
Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Cras sed risus
+
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">March 28th</div>
 +
<p>Put forward a kind of novel plant paint which can paint on the plant surface to absorb PM2.5.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">April 1st</div>
 +
<p>Catch the idea of yeast probiotic yogurt which helps people who allergic to alcohol to rapid degradation of ethanol.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">April 6th</div>
 +
<p>We had a brain storming: tracer showing the concentration of biomolecules, plants can emit colored light, new type of bacterial cell, and wastewater treatment by magnetotactic bacterium, etc.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">April 9th</div>
 +
<p>We established the iGEM club in our campus.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">April 20th</div>
 +
<p>Determined the project as "magnetotactic <i>E.coli</i> generates electronics."</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">April 22th</div>
 +
<p>Ascertained the origin of <i>mamW</i> gene in <i>MSR-1</i> genome.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">April 27th</div>
 +
<p>Ascertained the origin of <i>laccase</i> gene - derived from <i>Escherichia coli</i>.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">May 5th</div>
 +
<p>Designed the primers of <i>mamW</i>, <i>RFP</i> and <i>laccase</i>.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">May 7th</div>
 +
<p>Purchased the laboratory consumables and prepared for the first human practice activity.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">May 8th</div>
 +
<p>Communicated with professor Hu from Hong Kong University Medicine School.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">May 10th</div>
 +
<p>Determined three vectors for co-transferred which are pACYCDuet-1, pET28a and pCDFDuet-1.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">June 12th</div>
 +
<p>Amplified the <i>mamGFDC</i> and <i>mms6</i> with PCR from <i>MSR-1</i>.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">June 15th</div>
 +
<p>Linearization of the pACYCDuet-1 and amplification of the <i>mamW</i>, <i>RFP</i> and <i>laccase</i>.</p>
 
</div>
 
</div>
 
<div class="clear"></div>
 
<div class="clear"></div>
 
 
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">June 18th</div>
 +
<p>Presentation and science lesson at the experimental Kindergarden Affiliated to UESTC.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
<div class="exhib_cont fleft">
 
<div class="exhib_cont fleft">
−
<div class="exhib_date_right">2009</div>
+
<div class="exhib_date_right">June 19th</div>
−
<p>“Vestibulum euismod ”, Columbus College, Columbus, Ohio</p>
+
<p>Presentation and Interaction at the Primary School Affiliated to UESTC</p>
−
Donec in vehicula augue. Sed et nisi sem, at semper dolor. Pellentesque habitant morbi tristique senectus
+
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">July 15th</div>
 +
<p>Four teaching teams’ from iGEM club departure towards schools in remote area.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">July 21th</div>
 +
<p>Our summer vocation began, in order to have fun before the summer iGEM training, we go out for an outing!</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">July 25th</div>
 +
<p>Screened positive clones of <i>mamW</i> + <i>RFP</i> + <i>laccase</i> and <i>RFP</i> + <i>laccase</i>.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">July 21th</div>
 +
<p>The start of the Summer Training Camp.</p>
 
</div>
 
</div>
 
<div class="clear"></div>
 
<div class="clear"></div>
 
 
 
<div class="exhib_cont fright">
 
<div class="exhib_cont fright">
−
<div class="exhib_date_left">2008</div>
+
<div class="exhib_date_left">July 25th</div>
−
<p>“Aliquam vitae lorem ”, Junction View Studios, Columbus, Ohio</p>
+
<p>The establishment of the iGEM Southwest Union of China, also the first meet up.</p>
−
Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Cras sed risus
+
 
</div>
 
</div>
 
<div class="clear"></div>
 
<div class="clear"></div>
  
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">July 27th</div>
 +
<p>We transferred <i>mamW</i>, <i>RFP</i> and <i>laccase</i> gene into <i>Escherichia coli BL21 (DE3)</i> and induced its expression.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">July 30th</div>
 +
<p>Connected <i>mamAB</i> with the vector pET28a and complete the construction of vector piGEM-AB.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">August 5th</div>
 +
<p>Amplified the <i>mamXY</i> with PCR from <i>MSR-1</i>. Completed the construction of vector piGEM-AB.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">August 12th</div>
 +
<p>Communication with technical expert at Anno Medical Devices Co.Ltd.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">August 13th</div>
 +
<p>Detection of laccase activity using by the ABTS. Communication with Manager Zhiyong Xu at Jin Douyun Space Company.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">August 17th</div>
 +
<p>Finished the project video.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">August 23th</div>
 +
<p>Connected <i>mamXY</i> with the vector pCDFDuet-1 and connected <i>mamGFDC</i> + <i>mms6</i> with the vector pCDFDuet-1-mamXY.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">August 25th</div>
 +
<p>We visited Chengdu Biology Maker Space and got their technical support.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">August 29th</div>
 +
<p>We were excited to obtain the exact Parts including the <i>mamW</i> gene and mutanted it.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">August 30th</div>
 +
<p>We completed our enzymatic biofuel cell! It worked as we expected!</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">September 2nd</div>
 +
<p>The first time we presented our whole project presentation in front of all instructors and professors of our school.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">September 10th</div>
 +
<p>It is an exciting day that we sent our Parts to MIT, wish the little box bon voyage. Happy to complete the construction of cell device and start to test it.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">September 15th</div>
 +
<p>Team members gathered together to modify the wiki for the finalist version under the guidance of our instructors.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fright">
 +
<div class="exhib_date_left">September 18th</div>
 +
<p>We completed our wiki and poster, which means we completed the basic standards of medals. Next is the comprehensive preparation of presentation.</p>
 +
</div>
 +
<div class="clear"></div>
 +
 +
<div class="exhib_cont fleft">
 +
<div class="exhib_date_right">September 23th</div>
 +
<p>Set out for 2015 iGEM Giant Jamboree in MIT. We are coming, we are CHINA_CD_UESTC!</p>
 +
</div>
 +
<div class="clear"></div>
 +
 
</div>
 
</div>
 
</div>
 
</div>
−
<div class="clear"></div>
 
 
</div>
 
</div>
 
 

Latest revision as of 10:29, 18 September 2015

<!DOCTYPE html>

JOURNAL

  Hi! Here recorded the “significant” stages of our team which we experienced throughout the whole summer. In the initial establishment of our team, we organized various social activities and uneasy demonstrated the project at the first time, which all presents to you here.

Journal

March 24th

The establishment of our team!

March 26th

Came up with gas detector that examine institution and several diseases of our body.

March 28th

Put forward a kind of novel plant paint which can paint on the plant surface to absorb PM2.5.

April 1st

Catch the idea of yeast probiotic yogurt which helps people who allergic to alcohol to rapid degradation of ethanol.

April 6th

We had a brain storming: tracer showing the concentration of biomolecules, plants can emit colored light, new type of bacterial cell, and wastewater treatment by magnetotactic bacterium, etc.

April 9th

We established the iGEM club in our campus.

April 20th

Determined the project as "magnetotactic E.coli generates electronics."

April 22th

Ascertained the origin of mamW gene in MSR-1 genome.

April 27th

Ascertained the origin of laccase gene - derived from Escherichia coli.

May 5th

Designed the primers of mamW, RFP and laccase.

May 7th

Purchased the laboratory consumables and prepared for the first human practice activity.

May 8th

Communicated with professor Hu from Hong Kong University Medicine School.

May 10th

Determined three vectors for co-transferred which are pACYCDuet-1, pET28a and pCDFDuet-1.

June 12th

Amplified the mamGFDC and mms6 with PCR from MSR-1.

June 15th

Linearization of the pACYCDuet-1 and amplification of the mamW, RFP and laccase.

June 18th

Presentation and science lesson at the experimental Kindergarden Affiliated to UESTC.

June 19th

Presentation and Interaction at the Primary School Affiliated to UESTC

July 15th

Four teaching teams’ from iGEM club departure towards schools in remote area.

July 21th

Our summer vocation began, in order to have fun before the summer iGEM training, we go out for an outing!

July 25th

Screened positive clones of mamW + RFP + laccase and RFP + laccase.

July 21th

The start of the Summer Training Camp.

July 25th

The establishment of the iGEM Southwest Union of China, also the first meet up.

July 27th

We transferred mamW, RFP and laccase gene into Escherichia coli BL21 (DE3) and induced its expression.

July 30th

Connected mamAB with the vector pET28a and complete the construction of vector piGEM-AB.

August 5th

Amplified the mamXY with PCR from MSR-1. Completed the construction of vector piGEM-AB.

August 12th

Communication with technical expert at Anno Medical Devices Co.Ltd.

August 13th

Detection of laccase activity using by the ABTS. Communication with Manager Zhiyong Xu at Jin Douyun Space Company.

August 17th

Finished the project video.

August 23th

Connected mamXY with the vector pCDFDuet-1 and connected mamGFDC + mms6 with the vector pCDFDuet-1-mamXY.

August 25th

We visited Chengdu Biology Maker Space and got their technical support.

August 29th

We were excited to obtain the exact Parts including the mamW gene and mutanted it.

August 30th

We completed our enzymatic biofuel cell! It worked as we expected!

September 2nd

The first time we presented our whole project presentation in front of all instructors and professors of our school.

September 10th

It is an exciting day that we sent our Parts to MIT, wish the little box bon voyage. Happy to complete the construction of cell device and start to test it.

September 15th

Team members gathered together to modify the wiki for the finalist version under the guidance of our instructors.

September 18th

We completed our wiki and poster, which means we completed the basic standards of medals. Next is the comprehensive preparation of presentation.

September 23th

Set out for 2015 iGEM Giant Jamboree in MIT. We are coming, we are CHINA_CD_UESTC!