Difference between revisions of "Team:Birkbeck/Modeling"
Line 16: | Line 16: | ||
<table class="lamp"> | <table class="lamp"> | ||
+ | <table align="right"> | ||
<tr> | <tr> | ||
<th><img src="https://static.igem.org/mediawiki/2015/d/d4/Birkbeck_phage.png" height=200 width=100> | <th><img src="https://static.igem.org/mediawiki/2015/d/d4/Birkbeck_phage.png" height=200 width=100> |
Revision as of 11:03, 18 September 2015
Modelling
Modelling the directed evolution of bacteriophage lambda
To accomplish the goal of altering the host specificity of bacteriophage lambda, we took a step-by-step directed evolution approach, working according to the 'design - build - model - test - repeat' principle. This started with the design of several mutations both to alter the phage phenotype and to make segments of its genotype BioBrick-compatible. We then proceeded to 'build' individual genes through synthesis and cloning. The next steps are to recombine these genes into a recombinant bacteriophage genome, before carrying out testing and selection, and repeating this process.
The process is as follows: