Difference between revisions of "Team:Aachen/Notebook/Documentation/Methanol Monocistronic Diversity Library"
(→Assembly of MDH precursors) |
(→15-08-15) |
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==15-08-15== | ==15-08-15== | ||
− | ===Tasks:=== | + | ====Tasks:==== |
− | * | + | * purify plasmid of prouven clones from yesterday |
− | + | ||
* '''make masterplates of MDH precursors (new assembly, this time with blockers)''' | * '''make masterplates of MDH precursors (new assembly, this time with blockers)''' | ||
− | * assembly of | + | * assembly of hps and phi precursors (with blockers) |
* tidy up fridge | * tidy up fridge | ||
− | * | + | * do transformation of hps and phi precursors |
− | * | + | * do transformation of mdh.AP04 and mdh.AP10 precursors |
− | + | ||
− | + | ||
− | + | ||
− | ===Plasmid | + | ====Plasmid purification of hps and phi precursors==== |
{|class="wikitable" | {|class="wikitable" | ||
! construct !! Cryo ID !! Plasmid ID !! Plasmid conc. [ng/µl] | ! construct !! Cryo ID !! Plasmid ID !! Plasmid conc. [ng/µl] | ||
|- | |- | ||
− | | | + | | hps AP19 #14 || #FOF4# || #B3PQ# || 170 |
|- | |- | ||
− | | | + | | hps AP10 #14 || #638V# || #P4SB#|| 148 |
|- | |- | ||
− | | | + | | hps AP10 #11 || #4ZSB# || #P89F#|| 143,5 |
|- | |- | ||
− | | | + | | hps AP04 #9 || #NY1C# || #1TMT#|| 190,5 |
|- | |- | ||
− | | | + | | hps AP19 #15 || #SM3L# || #NFOS#|| 204 |
|- | |- | ||
− | | | + | | hps AP00 #15|| #HAKR# || #6ACO#|| 126,5 |
|- | |- | ||
− | | | + | | hps AP19 #13 || #COMY# || #VSWC#|| 261 |
|- | |- | ||
− | | | + | | hps AP19 #9 || #OZ4O# || #L8Y4#|| 171,5 |
|- | |- | ||
− | | | + | | hps AP10 #13 || #8P4S# || #4QLE#|| 238,5 |
|- | |- | ||
− | | | + | | hps AP19 #16|| #CSLZ# || #6P68#|| 121,5 |
|- | |- | ||
− | | | + | | phi AP10 #3 || #BV3E# || #L9E9#|| 161 |
|- | |- | ||
− | | | + | | phi AP10 #4 || #OFHN# || #FVVW#|| 171,5 |
|- | |- | ||
− | | | + | | phi AP19 #5 || #E6HX# || #HA1W#|| 192,5 |
|} | |} | ||
− | ===Assembly of | + | ===Assembly of hps precursors=== |
− | * this time with blockers ( | + | * this time with blockers (better coupling efficiency) |
− | **Assembly product | + | **Assembly product hps.AP00: #WQPR# |
− | **Assembly product | + | **Assembly product hps.AP04: #6WOE# |
− | **Assembly product | + | **Assembly product hps.AP10: #XHXQ# |
− | **Assembly product | + | **Assembly product hps.AP19: #VM1V# |
− | ===Assembly of | + | ===Assembly of phi precursors=== |
− | * this time with blockers ( | + | * this time with blockers (better coupling efficiency) |
− | **Assembly product | + | **Assembly product phi.AP00: #AFA4# |
− | **Assembly product | + | **Assembly product phi.AP04: #8FXO# |
− | **Assembly product | + | **Assembly product phi.AP10: #KCDE# |
− | **Assembly product | + | **Assembly product phi.AP19: #QHM6# |
− | === | + | ====Transformations==== |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
{|class="wikitable" | {|class="wikitable" | ||
! # !! description !! assembly product | ! # !! description !! assembly product | ||
|- | |- | ||
− | | 1 || | + | | 1 || hps.AP00 precursor || #WQPR# |
− | + | ||
− | + | ||
|- | |- | ||
− | | | + | | 2 || hps.AP04 precursor || #6WOE# |
|- | |- | ||
− | | | + | | 3 || hps.AP10 precursor || #XHXQ# |
|- | |- | ||
− | | | + | | 4 || hps.AP19 precursor || #VM1V# |
|- | |- | ||
− | | | + | | 5 || phi.AP00 precursor || #AFA4# |
|- | |- | ||
− | | | + | | 6 || phi.AP04 precursor || #8FXO# |
|- | |- | ||
− | | | + | | 7 || phi.AP10 precursor || #KCDE# |
|- | |- | ||
− | | | + | | 8 || phi.AP19 precursor || #QHM6# |
|- | |- | ||
− | | | + | | 9 || mdh.AP04 precursor || #VYM6# |
|- | |- | ||
− | | | + | | 10 || mdh.AP10 precursor || #Q9QQ# |
|} | |} | ||
Revision as of 12:23, 18 September 2015