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For confocal microscopy analysis, exosomes (100 μg) loaded with fluorescence-labelled siRNA were incubated with the Neuro2A cells (10<sup>6</sup> cells). After 6 hours, the cells were washed, fixed and observed under a confocal microscope (FV 1000; Olympus, Tokyo). Pictures were taken under the following conditions: objective lens: PLAPON 60 × O; NA: 1.42; scan mode: XY; excitation wavelength: 405 nm for Hoechst 33342 and 555 nm for Alexa Fluor 555; and image size: 1024 × 1024 pixels. </br> </br> | For confocal microscopy analysis, exosomes (100 μg) loaded with fluorescence-labelled siRNA were incubated with the Neuro2A cells (10<sup>6</sup> cells). After 6 hours, the cells were washed, fixed and observed under a confocal microscope (FV 1000; Olympus, Tokyo). Pictures were taken under the following conditions: objective lens: PLAPON 60 × O; NA: 1.42; scan mode: XY; excitation wavelength: 405 nm for Hoechst 33342 and 555 nm for Alexa Fluor 555; and image size: 1024 × 1024 pixels. </br> </br> | ||
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+ | Exosomes (100 μg) loaded with MOR siRNAs were incubated with Neuro2A cells (10<sup>6</sup> cells). After 24 h incubation, the recipient cells were collected for total RNA extraction and subsequent quantitative RT-PCR analysis of MOR siRNA and MOR mRNA, and for total protein isolation and subsequent western blotting analysis of MOR protein. | ||
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In CPP test, researchers place an animal in a distinctively designed chamber and inject or infuse it with the substance being studied (e.g., morphine in this study). Once trained to associate the chamber with the test agent, the animal is placed in an anteroom connecting a neutral chamber and the drug-associated chamber. The animal indicates its preferred chamber by spending more time there; its choice reveals the animal’s preference for or aversion to the test substance. </br> | In CPP test, researchers place an animal in a distinctively designed chamber and inject or infuse it with the substance being studied (e.g., morphine in this study). Once trained to associate the chamber with the test agent, the animal is placed in an anteroom connecting a neutral chamber and the drug-associated chamber. The animal indicates its preferred chamber by spending more time there; its choice reveals the animal’s preference for or aversion to the test substance. </br> |
Revision as of 17:42, 18 September 2015
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Notebook
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