Difference between revisions of "NJU-China-notebook.html"
Line 241: | Line 241: | ||
Exosome injection (Days 26-32): Before exosome injection, all mice underwent a CPP test. On days 26, 28, 30 and 32, mice were injected intravenously with saline, 200 µg exosomes loaded with MOR siRNA, or 200 µg RVG exosomes loaded with MOR siRNA, respectively.</br> | Exosome injection (Days 26-32): Before exosome injection, all mice underwent a CPP test. On days 26, 28, 30 and 32, mice were injected intravenously with saline, 200 µg exosomes loaded with MOR siRNA, or 200 µg RVG exosomes loaded with MOR siRNA, respectively.</br> | ||
Reinstatement (Days 33-34): On day 33, before being placed in the white chamber for 30 min, all mice received morphine (10 mg/kg). On day 34, all mice underwent a 30 min CPP test. </br></br> | Reinstatement (Days 33-34): On day 33, before being placed in the white chamber for 30 min, all mice received morphine (10 mg/kg). On day 34, all mice underwent a 30 min CPP test. </br></br> | ||
− | <img src="https://static.igem.org/mediawiki/2015/a/af/NJU-China-ForceSwimming-title.png" style="width: | + | <img src="https://static.igem.org/mediawiki/2015/a/af/NJU-China-ForceSwimming-title.png" style="width:900px"></br></br> |
C57BL/6J Mice (n=3 for each group) were randomly divided into control group and test group. Mice in the control group received saline injection and mice in the test group received exosome injection (200 μg RVG exosomes loaded with MOR siRNA). After two days, mice were subjected to two trials during which they were plunged individually and forced to swim in a tank (height 30 cm, length 50 cm, width 40 cm) filled with 15 cm of water maintained at 25 °C. The first training trial lasted 15 min and was performed one day before testing. Then, after 24 h, a second trial was performed and lasted for 10 min. The time that the test animal spent in the second trial without making any movements beyond those required to keep its head above water was measured and further analyzed. Both of two trials were videoed. | C57BL/6J Mice (n=3 for each group) were randomly divided into control group and test group. Mice in the control group received saline injection and mice in the test group received exosome injection (200 μg RVG exosomes loaded with MOR siRNA). After two days, mice were subjected to two trials during which they were plunged individually and forced to swim in a tank (height 30 cm, length 50 cm, width 40 cm) filled with 15 cm of water maintained at 25 °C. The first training trial lasted 15 min and was performed one day before testing. Then, after 24 h, a second trial was performed and lasted for 10 min. The time that the test animal spent in the second trial without making any movements beyond those required to keep its head above water was measured and further analyzed. Both of two trials were videoed. | ||
− | < | + | <img src="https://static.igem.org/mediawiki/2015/2/21/NJU-China-methods2.png",style="width:450px;float:left"> |
</div> | </div> | ||
Revision as of 18:12, 18 September 2015
|
Notebook
|