Difference between revisions of "Team:Cooper Union/Parts"

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{{Cooper_Union}}
 
 
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<li class="menu"> <a class="menu" href="https://2015.igem.org/Team:Cooper_Union"> Home </a> </li>
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<li class="menu-parts"> <a class="menu" href="https://2015.igem.org/Team:Cooper_Union/Parts">Biobrick Parts</a>
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<!-----Add content below---->
  
 
<h2> Part Documentation</h2>
 
<h2> Part Documentation</h2>
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
  
  
 
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<h4>Note</h4>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h3>BBa_K1746000 - TdT delta1-27 (N-terminal Truncated Variant of TdT)</h3>
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<p>This DNA sequence is a truncation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. This deletion was of a putative nuclear localization sequence. This sequence was demonstrated to show 80% catalytic activity relative to full length, untruncated, TdT in 293T cells. Expression of this sequence results in a protein that can be used for 3'-end non-templated synthesis of oligonucleotides.</p>
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<h3>BBa_K1746001 - TdT delta26-143 (Truncated TdT Variant)</h3>
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<p>This DNA sequence is a mutated variant of a sequence coding for the bovine terminal deoxynucleotidyltransferase (TdT) enzyme. The sequence was originally desinged by Repasky et. al in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. It includes a deletion of the 26th to 143rd amino acids of the naturally occurring TdT protein. It shows catalytic activity similar to that of full length TdT in 293T cells. It is intended to be used for adding nucleotides to the 3 prime end of a DNA sequence.</p>
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<h3>BBa_K1746002 - TdT GIP 213-215 subAAA (Mutated TdT Variant)</h3>
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<p>This DNA sequence is a mutation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. To create this sequence, amino acids 215-218 GIP, were substituted with AAA. This sequence was demonstrated to show 63% catalytic activity relative to full length, un-mutated, TdT. Expression of this sequence results in a protein that can be used for 3'-end non-templated synthesis of oligonucleotides.</p>
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<h4>Adding parts to the registry</h4>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
  
  
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<h4>What information do I need to start putting my parts on the Registry?</h4>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<ul>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ul>
 
  
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<p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
  
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<h4>Inspiration</h4>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
  
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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</ul>
 
  
  
  
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<h4>Part Table </h4>
 
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<groupparts>iGEM015 Example</groupparts>
 
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Latest revision as of 21:46, 18 September 2015

Cooper Union 2015 iGEM



Part Documentation

BBa_K1746000 - TdT delta1-27 (N-terminal Truncated Variant of TdT)

This DNA sequence is a truncation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. This deletion was of a putative nuclear localization sequence. This sequence was demonstrated to show 80% catalytic activity relative to full length, untruncated, TdT in 293T cells. Expression of this sequence results in a protein that can be used for 3'-end non-templated synthesis of oligonucleotides.

BBa_K1746001 - TdT delta26-143 (Truncated TdT Variant)

This DNA sequence is a mutated variant of a sequence coding for the bovine terminal deoxynucleotidyltransferase (TdT) enzyme. The sequence was originally desinged by Repasky et. al in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. It includes a deletion of the 26th to 143rd amino acids of the naturally occurring TdT protein. It shows catalytic activity similar to that of full length TdT in 293T cells. It is intended to be used for adding nucleotides to the 3 prime end of a DNA sequence.

BBa_K1746002 - TdT GIP 213-215 subAAA (Mutated TdT Variant)

This DNA sequence is a mutation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. To create this sequence, amino acids 215-218 GIP, were substituted with AAA. This sequence was demonstrated to show 63% catalytic activity relative to full length, un-mutated, TdT. Expression of this sequence results in a protein that can be used for 3'-end non-templated synthesis of oligonucleotides.